Czech J. Genet. Plant Breed., 2010, 46(4):178-182 | DOI: 10.17221/60/2010-CJGPB

Complete genome sequence of a Brome Mosaic Virus isolate from the Czech RepublicShort Communication

Sebastien Gadiou, Jiban Kumar Kundu
Department of Virology, Division of Plant Health, Crop Research Institute, Prague, Czech Republic

An isolate of Brome mosaic virus (BMV) was originally isolated from Agropyron repens and maintained in Hordeum vulgare. The full-length genome of this isolate (BMV-CZ) was sequenced. Phylogenetic analysis revealed that BMV-CZ shared a minimum of 95.6% sequence identity, localized in the 5'-UTR of RNA-1 with the other BMV isolates from the database, and a maximum of divergence of 30.8% with Broad bean mottle virus localized in the 5'-UTR of RNA-3. This is the first sequence report of full-length BMV from the Czech Republic.

Keywords: BMV; sequence analysis; variability

Published: December 31, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Gadiou S, Kundu JK. Complete genome sequence of a Brome Mosaic Virus isolate from the Czech Republic. Czech J. Genet. Plant Breed. 2010;46(4):178-182. doi: 10.17221/60/2010-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ahlquist P., Allison R., Dejong W., Janda M., Kroner P., Pacha R., Traynor P. (1990): Molecular-biology of Bromovirus replication and host specificity. In: Pirone T.P., Shaw J.G. (eds): Viral Genes and Plant Pathogenesis. Springer, New York, 144-155. Go to original source...
    2. Ahola T., Ahlquist P. (1999): Putative RNA capping activities encoded by Brome mosaic virus: methylation and covalent binding of guanylate by replicase protein 1a. Journal of Virology, 73: 10061-10069. Go to original source... Go to PubMed...
    3. Calhoun S.L., Rao A.L.N. (2008): Functional analysis of brome mosaic virus coat protein RNA-interacting domains. Archives of Virology, 153: 231-245. Go to original source... Go to PubMed...
    4. Choi Y.G., Rao A.L.N. (2000): Molecular studies on bromovirus capsid protein VII. Selective packaging of BMV RNA4 by specific N-terminal arginine residues. Virology, 275: 207-217. Go to original source... Go to PubMed...
    5. Kumar S., Tamura K., Nei M. (2004): MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5: 150-163. Go to original source... Go to PubMed...
    6. Nei M. (1987): Molecular Evolutionary Genetics. Columbia University Press, New York. Go to original source...
    7. Roossinck M.J., Bujarski J., Ding S.W., Hajimorad R., Hanada K., Scott S., Tousignant M. (2005): Family Bromoviridae. Elsevier Academic Press, San Diego, 1049-1058.
    8. Saitou N., Nei M. (1987): The Neighbor-Joining Method - a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406-425.
    9. Smirnyagina E., Lin N.S., Ahlquist P. (1996): The polymerase-like core of brome mosaic virus 2a protein, lacking a region interacting with viral 1a protein in vitro, maintains activity and 1a selectivity in RNA replication. Journal of Virology, 70: 4729-4736. Go to original source... Go to PubMed...
    10. Sullivan M.L., Ahlquist P. (1997): cis-acting signals in bromovirus RNA replication and gene expression: Networking with viral proteins and host factors. Seminars in Virology, 8: 221-230. Go to original source...
    11. Takeda A., Kaido M., Okuno T., Mise K. (2004): The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in longdistance movement. Journal of General Virology, 85: 1751-1761. Go to original source... Go to PubMed...
    12. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. (1997): The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876-4882. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content