Czech Journal of Genetics and Plant Breeding, 2010 (vol. 46), issue 3

Potential of chloroplast genome in plant breedingReview

Jana Řepková

Czech J. Genet. Plant Breed., 2010, 46(3):103-113 | DOI: 10.17221/79/2010-CJGPB  

Chloroplast engineering (or chloroplast transformation technology, CTT) is a strategy consisting of inserting a transgene into the chloroplast genome of a plant instead of its nuclear genome. CTT brings advantages such as control of the site of gene insertion, high rates of transgene expression and protein accumulation, lack of transmission of the transgene via pollen due to the fact that plastid genes are maternally inherited and an absence of epigenetic effects. Tobacco remains the species most amenable to CTT to date, although chloroplast genetic engineering has also been achieved successfully in crops such as maize, tomato, cotton, potato, rice...

Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Slovak Republic in 2005, 2006 and 2008Original Paper

A. Hanzalová, J. Huszár, E. Herzová, P. Bartoš

Czech J. Genet. Plant Breed., 2010, 46(3):114-121 | DOI: 10.17221/38/2010-CJGPB  

In 2005, 2006 and 2008 the virulence of wheat leaf rust population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (141 in total) were obtained from different parts of Slovakia. Resistance gene Lr9 was effective to all tested isolates except three isolates from 2008. No virulence was found to Lr19 and genes Lr24 and Lr28 were also highly effective. Low incidence of virulence to Lr2a was observed. Sixty-five winter wheat cultivars registered in Slovakia were tested with...

Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht AllelesOriginal Paper

Jana Chrpová, Václav Šíp, Lenka Štočková, Zbyněk Milec, Ludmila Bobková

Czech J. Genet. Plant Breed., 2010, 46(3):122-134 | DOI: 10.17221/74/2010-CJGPB  

Resistance of 31 winter wheat varieties (bred in 6 European countries) to Fusarium head blight (FHB) was evaluated in field trials lasting for three years (2007, 2008, 2009) after artificial inoculation with Fusarium culmorum. The data on deoxynivalenol (DON) content were supplemented by symptom scores and determination of % of Fusarium damaged grains and % reductions of thousand-grain weight and grain weight per spike due to infection. These varieties and also 120 advanced breeding lines from the Úhřetice Breeding Station, SELGEN company were examined for the presence of gibberellic acid (GA) insensitive semi-dwarfing genes to evaluate their...

Study of relationships among twelve Phyllanthus species with the use of molecular markersOriginal Paper

Gyana Ranjan Rout, Sunil K. Senapati, S. Aparajita

Czech J. Genet. Plant Breed., 2010, 46(3):135-141 | DOI: 10.17221/74/2009-CJGPB  

The present investigation was undertaken to describe the relationships among twelve species of Phyllanthus collected in India by help of molecular markers. In total, 259 marker loci were assessed, out of which 249 were polymorphic revealing 96.13% polymorphism. Nei's similarity index varied from 0.35 to 0.76 for RAPD (Random Amplified Polymorphic DNA) and from 0.31 to 0.76 for ISSR marker systems. Cluster analysis by the unweighted pair group method (UPGMA) of Dice coefficient of similarity generated dendrogram with more or less similar topology for both the analyses that offered a better explanation for diversity and affinities between the...

Winter wheat SeladonNew Varieties

P. Horčička, O. Veškrna, T. Sedláček

Czech J. Genet. Plant Breed., 2010, 46(3):142-144 | DOI: 10.17221/101/2010-CJGPB