Czech J. Genet. Plant Breed., 2010, 46(3):122-134 | DOI: 10.17221/74/2010-CJGPB

Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht AllelesOriginal Paper

Jana Chrpová1, Václav Šíp1, Lenka Štočková1, Zbyněk Milec1, Ludmila Bobková2
1 Crop Research Institute, Prague-Ruzyně, Czech Republic
2 SELGEN, Breeding Station Úhřetice, Czech Republic

Resistance of 31 winter wheat varieties (bred in 6 European countries) to Fusarium head blight (FHB) was evaluated in field trials lasting for three years (2007, 2008, 2009) after artificial inoculation with Fusarium culmorum. The data on deoxynivalenol (DON) content were supplemented by symptom scores and determination of % of Fusarium damaged grains and % reductions of thousand-grain weight and grain weight per spike due to infection. These varieties and also 120 advanced breeding lines from the Úhřetice Breeding Station, SELGEN company were examined for the presence of gibberellic acid (GA) insensitive semi-dwarfing genes to evaluate their effect on FHB. The conditions of experimental years highly influenced the performance of all characters. The highest production of DON occurred in 2008 at a high temperature and high moisture content during the infection period. In all years the moderate resistance to accumulation of DON (at the level of Swiss variety Arina) was detected in the Czech varieties Bakfis, Federer, Baletka, Samanta and Sakura. Among these varieties, Federer showed a low accumulation of DON at a relatively higher symptom expression and greater reduction of grain weight per spike, but the other four varieties and the reference variety Arina expressed resistance in all the examined traits. The varieties Pitbull, Cubus, Kodex and Bagou were found to be highly susceptible to FHB. The presence of the dwarfing allele Rht-D1b resulted in a significantly higher mean symptom score and also in a higher affection of the other traits. Nonetheless, the analysis of frequency distributions in symptom scores showed the presence of resistant lines also among the GA insensitive lines, but with a lower frequency than in the group of GA sensitive genotypes. A relatively greater effect on manifestation of the disease had plant height, and therefore it is suggested that the adverse impact of Rht-D1b on FHB resistance could be to a high degree excluded by opting for taller Rht-D1b genotypes.

Keywords: deoxynivalenol content; disease severity traits; dwarfing allele Rht-D1b; Fusarium culmorum; head blight; plant height, variety resistance; winter wheat

Published: September 30, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Chrpová J, Šíp V, Štočková L, Milec Z, Bobková L. Resistance of winter wheat varieties registered in the Czech Republic to Fusarium head blight in relation to the presence of specific Rht Alleles. Czech J. Genet. Plant Breed. 2010;46(3):122-134. doi: 10.17221/74/2010-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akinsanmi O.A., Backhouse D., Simpfendorfer S., Chakraborty S. (2006): Pathogenic variation of Fusarium isolates associated with head blight of wheat in Australia. Journal of Phytopathology, 154: 513-521. Go to original source...
    2. Bai G.H., Shaner G. (1996): Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Disease, 80: 975-979. Go to original source...
    3. Bai G.H., Plattner R., Desjardins A., Kolb F.L. (2001): Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding, 120: 1-6. Go to original source...
    4. Bobková L., Šíp V., Škorpík M. (1988): The use of the method of Rht genes detection by means of applied gibberellin in wheat breeding. Genetika a Šlechtění, 24: 65-74. (in Czech)
    5. Buerstmanyr H., Lemmens M., Hartl L., Doldi L., Steiner B., Stierschneider M., Ruckenbauer P. (2002): Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat: Heritability and trait associations. Crop Science, 40: 1012-1018. Go to original source...
    6. Chrpová J., Šíp V., Matějová E., Sýkorová S. (2007): Resistance of winter wheat varieties registered in the Czech Republic to mycotoxin accumulation in grain following inoculation with Fusarium culmorum. Czech Journal of Genetics and Plant Breeding, 43: 44-52. Go to original source...
    7. Cöster H. (2010): Molekulare Marker in der praktischen Weizenzűchtung bei RAGT. In: Tagungsband der 60. Jahrestagung der Vereinigung der Pflanzenzűchter und Saatgutkaufleute Österreichs 2009, 57-60.
    8. D´Mello J.P.F., Placinta C.M., Macdonald A.M.C. (1999): Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Animal Feed Science and Technology, 80: 183-205. Go to original source...
    9. Draeger R., Gosman N., Steed A., Chandler E., Thomsett M., Srinivasachary, Schondelmaier J., Buerstmayr H., Lemmens M., Schmolke M., Mesterhazy A., Nicholson P. (2007): Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theoretical and Applied Genetics, 115: 617-625. Go to original source... Go to PubMed...
    10. Ellis M.H., Spielmeyer W., Gale K.R., Rebetzke G.J., Richards R.A. (2002): :"Perfect" markers for the RhtB1b and Rht-D1b dwarfing genes in wheat. Theoretical and Applied Genetics, 105: 1038-1042. Go to original source... Go to PubMed...
    11. Gale M.D., Gregory R.S. (1977): A rapid method for early generation selection of dwarf genotypes in wheat. Euphytica, 26: 733-738. Go to original source...
    12. Gervais L., Dedryver F., Morlais J.-Y., Bodusseau V., Negre S., Bilous M., Groos C., Trottet M. (2003): Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theoretical and Applied Genetics, 106: 961-970. Go to original source... Go to PubMed...
    13. Gosman N., Bayles R., Jennings P., Kirby J., Nicholson P. (2007): Evaluation and characterization of resistance to fusarium head blight caused by Fusarium culmorum in UK winter wheat cultivars. Plant Pathology, 56: 264-276. Go to original source...
    14. Hollins T.W., Ruckenbauer P., DeJong H. (2003): Progress towards wheat varieties with resistance to Fusarium head blight. Food Control, 14: 239-244. Go to original source...
    15. Holzapfel J., Voss H.-H., Miedaner T., Korzun V., Häberle J., Schweizer G., Mohler V., Zimmermann G., Hartl L. (2008): Inheritance of resistance to Fusarium head blight in three European winter wheat populations. Theoretical and Applied Genetics, 117: 1119-1128. Go to original source... Go to PubMed...
    16. Horčička P., Hanišová A., Chrpová J. (2007): Winter wheat Sakura. Czech Journal of Genetics and Plant Breeding, 43: 149-155. Go to original source...
    17. Jenny E., Hecker A., Kessler P., Kulling C., Forrer H.R. (2000): Getreidefusariosen: Sortenresistenz and Toxingehalte. Agrarforschung, 7: 270-273.
    18. Klahr A., Zimmermann G., Wenzel G., Mohler V. (2007): Effects of environment, disease progress, plant height and heading date on the detection of QTLs for resistance to Fusarium head blight in a European winter wheat cross. Euphytica, 154: 17-28. Go to original source...
    19. Kosová K., Chrpová J., Šíp V. (2009): Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding. Czech Journal of Genetics and Plant Breeding, 45: 87-105. Go to original source...
    20. Laml P., Pánek J. (2008): Winter wheat Bakfis. Czech Journal of Genetics and Plant Breeding, 44: 169-170. Go to original source...
    21. Laml P., Pánek J. (2010): Winter wheat Federer. Czech Journal of Genetics and Plant Breeding, 46: 97-98. Go to original source...
    22. Mathews K.L., Chapman S.C., Trethowan R., Singh R.P., Crossa J., Pfeiffer W., van Ginkel M., DeLacy I. (2006): Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes. Crop Science, 46: 603-613. Go to original source...
    23. Mesterházy A. (1995): Types and components of resistance to Fusarium head blight on wheat. Plant Breeding, 144: 377-386. Go to original source...
    24. Mesterházy A., Bartók T., Kászonyi G., Varga M., Tóth B., Varga J. (2005): Common resistance to different Fusarium spp. causing Fusarium head blight in wheat. European Journal of Plant Pathology, 112: 267-281. Go to original source...
    25. Mesterházy Á., Tóth B., Bartók T., Varga M. (2008): Breeding strategies against FHB in winter wheat and their relation to type I resistance. Cereal Research Communications, 36B: 37-43. Go to original source...
    26. Schmolke M., Zimmermann G., Buerstmayr H., Schweizer G., Miedaner T., Korzun V., Ebmeyer E., Hartl L. (2005): Molecular mapping of Fusarium head blight resistance in the winter wheat population Dream/Lynx. Theoretical and Applied Genetics, 111: 747-756. Go to original source... Go to PubMed...
    27. Schroeder H.W., Christensen J.J. (1963): Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology, 53: 831-838.
    28. Šíp V., Amler P., Bobková L., Škorpík M. (1988): Efficiency of early generation selection for Rht2 in a Czechoslovak wheat breeding programme. In: Miller T.E., Koebner R.M.D. (eds): Proc. 7th Int. Wheat Genetics Symposium. Vol. 2., Cambridge, 1175-1180.
    29. Šíp V., Sýkorová S., Stuchlíková E., Chrpová J. (2002): The effect of infection with Fusarium culmorum L. on deoxynivalenol content in grain of selected winter wheat varieties. Journal of Applied Genetics, 43A: 319-332.
    30. Šíp V., Chrpová J., Leišová L., Sýkorová S., Kučera L., Ovesná J. (2007): Effects of genotype, environment and fungicide treatment on development of Fusarium head blight and accumulation of DON in winter wheat grain. Czech Journal of Genetics and Plant Breeding, 43: 16-31. Go to original source...
    31. Šíp V., Chrpová J., Sýkorová S. (2008): Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolates. Czech Journal of Genetics and Plant Breeding, 44: 43-59. Go to original source...
    32. Šíp V., Chrpová J., Veškrna O., Bobková L. (2010): The impact of cultivar resistance and fungicide treatment on mycotoxin content in grain and yield losses caused by Fusarium head blight in wheat. Czech Journal of Genetics and Plant Breeding, 46: 21-26. Go to original source...
    33. Srinivasachary, Gosman N., Steed A., Simmonds J., Leverington-Waite M., Wang Y., Snape J., Nicholson P. (2008): Susceptibility to Fusarium head blight is associated with the Rht-D1b semi-dwarfing allele in wheat. Theoretical and Applied Genetics, 116: 1145-1153. Go to original source... Go to PubMed...
    34. Srinivasachary, Gosman N., Steed A., Hollins T.W., Bayles R., Jennings P., Nicholson P. (2009): Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight. Theoretical and Applied Genetics, 118: 695-702. Go to original source... Go to PubMed...
    35. Stack R.W., Frohberg R.C., Caspe H. (1997): Reaction of spring wheats incorporating Sumai-3 derived resistance to inoculation with seven Fusarium species. Cereal Research Communications, 25: 667-671. Go to original source...
    36. Steiner B., Lemmens M., Griesser M., Scholz U., Schondelmaier J., Buerstmayr H. (2004): Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theoretical and Applied Genetics, 109: 215-224. Go to original source... Go to PubMed...
    37. Voss H.-H., Holzapfel J., Hartl L., Korzun V., Rabenstein F., Ebmeyer E., Coester H., Kempf H., Miedaner T. (2008): Effect of the Rht-D1 dwarfing locus on Fusarium head blight rating in three segregating populations of winter wheat. Plant Breeding, 127: 333-339. Go to original source...
    38. Wiśniewska H., Perkowski J., Kaczmarek Z. (2004): Scab response and deoxynivalenol accumulation in spring wheat kernels of different geographical origins following inoculation with Fusarium culmorum. Journal of Phytopathology, 152: 613-621. Go to original source...
    39. Zadoks J.C., Chang T.T., Konzak C.F. (1974): Decimal code for growth stages of cereals. Weed Research, 15: 415-421. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content