Czech Journal of Genetics and Plant Breeding, 2005 (vol. 41), issue 3
Molecular Mapping in Barley: Shifting from the Structural to the Functional Level
A Graner, T Thiel, H Zhang, E Potokina, M Prasad, D Perovic, R Kota, R K Varshney, U Scholz, I Grosse, N Stein
Czech J. Genet. Plant Breed., 2005, 41(3):81-88 | DOI: 10.17221/3665-CJGPB 
As a resource for structural and functional barley genome analysis, more than 140 000 ESTs (expressed sequence tags) were generated from 22 cDNA libraries that yielded 25 224 tentative unigenes. About 50% of them belong to gene families. The size of the complete transcriptome is estimated to comprise between 35 000 and 75 000 genes. The barley EST collection is a rich source for the development of novel markers including SSRs (simple sequence repeats) and SNPs (single nucleotide polymorphisms). Several bioinformatic tools have been developed facilitating the computer-assisted analysis of EST databases for the presence of either SNPs or SSRs and the...
Principal Component Analysis of Near Infrared Spectra as a Tool of Endosperm Mutant Characterisation and in Barley Breeding for Quality
Lars MUNCK, Birthe MØLLER
Czech J. Genet. Plant Breed., 2005, 41(3):89-95 | DOI: 10.17221/3666-CJGPB
Near infrared technology, now widespread in quality control, makes it possible to obtain a total multivariate physical chemical fingerprint of the barley endosperm with high precision. Whole spectroscopic fingerprints of the physics and chemistry of barley seeds can be interpreted by multivariate analysis (chemometrics), by Principal Component Analysis (PCA) for classification and Partial Least Squares Regression (PLSR) for correlation. PCA classification of Near Infrared Reflectance (NIR) spectra can differentiate between mutants and alleles in the lys3 and lys5 loci. PCA on NIR can also be used as a routine in barley breeding to...
Grains of Nontraditional Crops as Sources of Retrograded Resistant Starch
D Mikulíková, I Čičová, G Antalíková, J Kraic
Czech J. Genet. Plant Breed., 2005, 41(3):96-104 | DOI: 10.17221/3667-CJGPB
The content of retrograded resistant starch of the RS3 type was determined in six plant species and their accessions. Besides sorghum and foxtail millet, plant genotypes were cultivated in Piešťany locality in the year 2003. There were significant differences in RS3 and total starch content in analysed crops and their accessions. Chickpea and buckwheat had the highest average content of RS3, 5.2% and 3.8%, respectively. Millet and foxtail millet contained less RS3, 2.9% and 2.8%, respectively. Millet was rich in total starch. The lowest amounts of RS3 were detected in sorghum (1.6%) and amaranth...
Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding
R Koprna, V Kučera, O Kolovrat, M Vyvadilová, M Klíma
Czech J. Genet. Plant Breed., 2005, 41(3):105-111 | DOI: 10.17221/3668-CJGPB
Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants,...
Use of Detached Seedling Leaf Test to Evaluate Wheat Resistance to Septoria Tritici Blotch
L. Věchet, M. Vojáčková
Czech J. Genet. Plant Breed., 2005, 41(3):112-116 | DOI: 10.17221/3669-CJGPB
The Czech Bank Of Potato Genetic Resources
V. Horáčková, J. Domkářová
Czech J. Genet. Plant Breed., 2005, 41(3):117-119 | DOI: 10.17221/6078-CJGPB
Memorandum Of The Participants Of The Seminar "New Knowledge In Plant Biology And Its Use In Solving The Needs Of Plant Breeding", Organised In Response To The European Biotechnology Platform "Plants For The Future"
editors
Czech J. Genet. Plant Breed., 2005, 41(3):120-121 | DOI: 10.17221/6079-CJGPB
Spring Barley Bojos
P. Svačina
Czech J. Genet. Plant Breed., 2005, 41(3):122-123 | DOI: 10.17221/6080-CJGPB
Spring Barley Radegast
P. Svačina
Czech J. Genet. Plant Breed., 2005, 41(3):124-125 | DOI: 10.17221/6081-CJGPB
Common Bentgrass Polana
I. Houdek
Czech J. Genet. Plant Breed., 2005, 41(3):126 | DOI: 10.17221/6082-CJGPB