Czech J. Genet. Plant Breed., 2005, 41(3):105-111 | DOI: 10.17221/3668-CJGPB

Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

R Koprna1, V Kučera2, O Kolovrat1, M Vyvadilová2, M Klíma2
1 OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech Republic
2 Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.

Keywords: oilseed rape (Brassica napus L. var. napus); hybrid breeding; self-incompatibility; seed quality; glucosinolates; erucic acid

Published: September 30, 2005  Show citation

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Koprna R, Kučera V, Kolovrat O, Vyvadilová M, Klíma M. Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding. Czech J. Genet. Plant Breed. 2005;41(3):105-111. doi: 10.17221/3668-CJGPB.
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