Czech Journal of Genetics and Plant Breeding, 2003 (vol. 39), issue 4
Index of Volume 39Index
editors
Czech J. Genet. Plant Breed., 2003, 39(4):X1 | DOI: 10.17221/6104-CJGPB 
Genealogical Analysis of the Genetic Diversity in Winter Wheat Cultivars Grown in the Former Czechoslovakia and the Present Czech Republic during 1919-2001
Z. Stehno, L. Dotlačil, I. Faberová, S. Martynov, T. Dobrotvorskaya
Czech J. Genet. Plant Breed., 2003, 39(4):99-108 | DOI: 10.17221/3726-CJGPB
Using genealogical analysis, the genetic diversity in winter wheat cultivars registered and grown in the formerCzechoslovakia and the presentCzech Republic during 1919-2001 was studied. The strong increase of the diversity level since the 1970-s is based on the wide use in breeding programs of foreign materials, most of which originated in countries of Western, Central andEastern Europe. Simultaneously a genetic erosion in the released cultivars occurred; from the 1930-s to the 1970-s, a significant number of original local ancestors was lost. The modern cultivars listed in the Czech National List of Varieties in 2000-2001 can be distinguished into...
SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model
J. Ovesná, K. Poláková, L. Kučera, J. Rulcová
Czech J. Genet. Plant Breed., 2003, 39(4):109-112 | DOI: 10.17221/3727-CJGPB
We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser.
RAPD Analysis of peaches within Czech National collection
J. Raddová, M. Beránek, I. Oukropec, M. Vachůn
Czech J. Genet. Plant Breed., 2003, 39(4):113-119 | DOI: 10.17221/3728-CJGPB
The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity and relationships within the collection of the Czech National Plant Genetic Resources (PGR) of peaches (Prunus persica L.). The aim of the work was to elaborate a dendrogram of genetic similarity and to divide collection into clusters. 46 primers were applied to 6 cultivars differing in the place of origin, the fruit shape, the fruit colour, and in some other morphological characteristics. 12 primers were chosen which gave polymorphic repeatable strong and middle strong bands. They were subsequently used for the RAPD reactions within the whole...
Utilisation of SSRs for Characterisation of the Soybean (Glycine max (L.) Merr.) Genetic Resources
M. Hudcovicová, J. Kraic
Czech J. Genet. Plant Breed., 2003, 39(4):120-126 | DOI: 10.17221/3729-CJGPB
The SSR profiles of 67 soybean genotypes of various origins have been detected by 188 alleles at the 18 SSR loci. From 4 to 21 alleles were found at each of these loci (average 10.4 per locus) and the gene diversity averaged 0.71. Differentiation of all 67 genotypes each from others has been successful by using of even only 6 of SSR markers (Sat001, Satt005, Satt038, Satt173, Satt177, Satt534) with gene diversity from 0.66 to 0.89. The cumulated probability of obtaining identical soybean SSR profile was 1.11 × 10-9 , which confirms a high potential of SSRs for differentiation of soybean accessions in collections. Clustering...
Diagnostic of Wheat Leaf Rust Resistance Genes by DNA Markers and their Application in MAS - Short Communication
Ž. Gregáňová, J. Kraic, Z. Gálová
Czech J. Genet. Plant Breed., 2003, 39(4):127-129 | DOI: 10.17221/3730-CJGPB
Leaf rust caused by Puccinia triticina belongs to the most important wheat diseases in Europe, including Slovakia. Winter wheat varieties registered in Slovakia have been tested with already developed STS (Lr9, Lr24), SCAR (Lr35) and SSR (Lr13) markers which are linked to the mentioned leaf rust resistance genes. Each of the four DNA markers linked to the individual resistance gene can be detected safely, easily and relatively fast by using the PCR reaction. This is very important for marker assisted selection (MAS) for the incorporation of the Lr9, Lr24 a Lr35 genes into chosen wheat genotypes...
Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape - Short Communication
J. Horáček, M. Ačanová
Czech J. Genet. Plant Breed., 2003, 39(4):130-133 | DOI: 10.17221/3731-CJGPB
In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to...
Rex B. - Breeding for quantivative traits in plants - Book Review
K. Vaculová
Czech J. Genet. Plant Breed., 2003, 39(4):134-135 | DOI: 10.17221/6103-CJGPB