Czech J. Genet. Plant Breed., 2003, 39(4):127-129 | DOI: 10.17221/3730-CJGPB
Diagnostic of Wheat Leaf Rust Resistance Genes by DNA Markers and their Application in MAS - Short Communication
- Department of Biochemistry and Biotechnology, Slovak Agricultural University in Nitra, Nitra, Slovak Republic; 2Department of Applied Biochemistry and Molecular Biology, Research Institute of Plant Production, Pie¹»any, Slovak Republic
Leaf rust caused by Puccinia triticina belongs to the most important wheat diseases in Europe, including Slovakia. Winter wheat varieties registered in Slovakia have been tested with already developed STS (Lr9, Lr24), SCAR (Lr35) and SSR (Lr13) markers which are linked to the mentioned leaf rust resistance genes. Each of the four DNA markers linked to the individual resistance gene can be detected safely, easily and relatively fast by using the PCR reaction. This is very important for marker assisted selection (MAS) for the incorporation of the Lr9, Lr24 a Lr35 genes into chosen wheat genotypes with good bread-making quality or for the characterisation of wheat genetic resources (Lr13 gene).
Keywords: Lr genes; PCR; wheat; Puccinia triticina; MAS
Published: December 31, 2003 Show citation
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