Czech Journal of Genetics and Plant Breeding, 2003 (vol. 39), issue 3
DNA Markers for High Molecular weight Glutenin Subunits 5+10 Used in Wheat and Triticale Breeding
M. Vinterová, J. Bednář, I. Ježíšková, P. Martinek
Czech J. Genet. Plant Breed., 2003, 39(3):69-72 | DOI: 10.17221/3722-CJGPB 
Prediction of flour breadmaking quality was verified using DNA markers in seven genotypes of winter wheat (T. aestivum L., 2n = 6x = 42, AABBDD) of different quality classes, four genotypes of triticale (× Triticosecale Wittmack, 2n = 6x = 42, AABBRR), and selected progenies of triticale Presto with the T1R.1D5+10-2 translocation. DNA isolated from fresh leaves (the stage of the first true leaf) was used to detect the Glu D1 5+10 allele based on the SPLAT protocol according to D'Ovidio and Anderson (1994). The presence of the Glu D1 5+10 allele was verified using a product...
Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien
O. Horňáková, M. Závodná, M. Žáková, J. Kraic, F. Debre
Czech J. Genet. Plant Breed., 2003, 39(3):73-83 | DOI: 10.17221/3723-CJGPB
The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics....
Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.)
L. Klčová, M. Gubišová
Czech J. Genet. Plant Breed., 2003, 39(3):84-88 | DOI: 10.17221/3724-CJGPB
In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H2SO4 and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not...
Detection of Norin 10 dwarfing genes in winter wheat varieties registered in the Czech Republic
J. Chrpová, M. Škorpík, P. Prášilová, V. Šíp
Czech J. Genet. Plant Breed., 2003, 39(3):89-92 | DOI: 10.17221/3725-CJGPB
Insensitivity to the applied gibberellic acid, indicating the presence of Norin 10 dwarfing genes, was detected in 20 of 57 (35.1%) winter wheat varieties registered in the Czech Republic in 2002. Hybrid analyses in F2 generation showed a prevalent occurrence of Rht2 gene (in 14 varieties). Only 6 varieties were found to carry Rht1 gene. Among the recently developed Rht2 varieties, Clever possesses also a high bread-making quality which was not observed with the older materials. Rht2 varieties were found, on average, less winter-hardy, later in heading and more resistant to lodging than Rht1 varieties.
100 years of plant breeding in Chlumec on Cidlina - Information
V. Tyller
Czech J. Genet. Plant Breed., 2003, 39(3):93-95 | DOI: 10.17221/6101-CJGPB
Winter wheat Meritto
L. Bobková
Czech J. Genet. Plant Breed., 2003, 39(3):97-98 | DOI: 10.17221/6102-CJGPB