Czech J. Genet. Plant Breed., 2003, 39(3):69-72 | DOI: 10.17221/3722-CJGPB

DNA Markers for High Molecular weight Glutenin Subunits 5+10 Used in Wheat and Triticale Breeding

M. Vinterová, J. Bednář, I. Ježíšková, P. Martinek
1 Czech Republic
2 Agricultural Research Institute Kroměříž, Ltd., Kroměříž, Czech Republic

Prediction of flour breadmaking quality was verified using DNA markers in seven genotypes of winter wheat (T. aestivum L., 2n = 6x = 42, AABBDD) of different quality classes, four genotypes of triticale (× Triticosecale Wittmack, 2n = 6x = 42, AABBRR), and selected progenies of triticale Presto with the T1R.1D5+10-2 translocation. DNA isolated from fresh leaves (the stage of the first true leaf) was used to detect the Glu D1 5+10 allele based on the SPLAT protocol according to D'Ovidio and Anderson (1994). The presence of the Glu D1 5+10 allele was verified using a product of 450 bp size. It was detected in the wheat genotypes Athlet, Brea, Bruneta, Iris, Lívia, Mona, Sida, and in all analysed progenies derived from the Presto triticale sample with the T1R.1D5+10-2 translocation. Effects are discussed of other loci on the final breadmaking quality in the wheat varieties Athlet, Lívia, and Mona with Glu D1 5+10 and a lower grain breadmaking quality.

Keywords: triticale; wheat; SPLAT; Glu D1 5+10; translocation; T1R.1D

Published: September 30, 2003  Show citation

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Vinterová M, Bednář J, Ježíšková I, Martinek P. DNA Markers for High Molecular weight Glutenin Subunits 5+10 Used in Wheat and Triticale Breeding. Czech J. Genet. Plant Breed. 2003;39(3):69-72. doi: 10.17221/3722-CJGPB.
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    References

    1. D'O R., A O.D. (1994): PCR analysis to distinguish between alleles of member of a multigene family correlated with bread-making quality. Theor. Appl. Genet., 88: 759-763. Go to original source... Go to PubMed...
    2. J D., B J., V T., M P., K A. (2002): Detection of locus GLU D1 5+10 in wheat gene resources with MRS and LG morphotypes of spike. In: Sustainable development of agriculture, preservation of landscape and biodiversity. In: Proc. Int. Sci. Conf. on the occasion of the 55th anniversary of the Slovak Agricultural University in Nitra. June 5- 6, 2001, Acta Fytotech. Zootech., 4: 289-291.
    3. L A.J. (1994): Genetic mapping in the 1R.1D wheat-rye translocated chromosome. Genome, 37: 6: 945-949. Go to original source... Go to PubMed...
    4. L A.J. (1998): Improvement of breadmaking quality of triticale through chromosome translocations. In: Proc. 4th Int Triticale Symp., July 26-31, Canada: 102-110.
    5. Š A., Č J. (1998): Elektroforetická spektra gliadinů a VMH podjednotek gluteninu odrůd pšenice obecné, registrovaných v letech 1996 a 1997. Czech J. Genet. Plant Breed., 34: 95-101.
    6. Š A., Č J., S S., B J. (2000): Inovované katalogy bílkovinných markerů pšenice seté a ječmene. ÚZPI, Praha.
    7. V P. (1998): Využití genetických markerů pro tvorbu dihaploidní pšenice obecné (Triticum aestivum L.). [Disertatační práce.] ČZU, Praha: 344s.
    8. W H., M R.J., L A.J., C A. (2002): The effect of the D-genome chromosome substitutions and translocations of chromosome 1D on some quality and agronomic parameters of winter triticale. In: Proc. 5th Int. Triticale Symp., June 30-July 5, 2002, Radzików, Poland, Vol. II - Poster Presentations: 59-70.

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