Czech Journal of Genetics and Plant Breeding, 2003 (vol. 39), issue 2

Resistance of Spring Barley Varieties to Powdery Mildew in the Czech Republicin 1971-2000

A. Dreiseitl, P. Pařízek

Czech J. Genet. Plant Breed., 2003, 39(2):31-44 | DOI: 10.17221/3718-CJGPB  

Resistance to powdery mildew of 127 spring barley varieties was evaluated in 702 official variety trials, using scores from 1 to 9. Trials with sufficient disease severity were only analysed. Varieties possessing the resistance genes Mla7 (Elgina), Ml(Kr) (BR-1519), Mla13 (Koral) and mlo (Forum) were among the most resistant ones. The varieties Diamant, HE-3527, HE-3631, II/61-FUDII and Zefir showed high susceptibility. Fifteen varieties, carrying the genes Mla1, Mla3, Mla6, Mla9, Mla13, Ml(Kr) and Ml(Sc), were in the first years of testing highly resistant,...

Use of Prolamin Polymorphism to Describe Genetic Variation in a Collection of Barley Genetic Resources

T. Vyhnánek, J. Bednář, S. Helánová, L. Nedomová, J. Milotová

Czech J. Genet. Plant Breed., 2003, 39(2):45-50 | DOI: 10.17221/3720-CJGPB  

The polymorphism of prolamin storage proteins was studied in seed samples of 20 historical cultivars of spring barley (Hordeum vulgare L.) of Czech and Slovak origin, using polyacrylamide gel electrophoresis (PAGE). Only two samples were uniform. Most heterogeneity of prolamin patterns was observed in the oldest accessions. By means of a prolamin identity index it was possible to distinguish sister lines from admixtures within the seed samples. The obtained spectra will be used as additional descriptors for the spring barley core collection of the Collection of Genetic Resources of the Agricultural Research Institute Kroměříž, Ltd.

Identification of Glutenin Markers in Cultivars of three Wheat Species

M. Starovičová, Z. Gálová, H. Knoblochová

Czech J. Genet. Plant Breed., 2003, 39(2):51-57 | DOI: 10.17221/3719-CJGPB  

In a collection of 41 Slovak and European winter wheat cultivars (Triticum aestivum L.), 5 spelt wheat cultivars (Triticum spelta L.) and 3 durum wheat cultivars (Triticum durum DESF.) we investigated the qualitative composition of high-molecular-weight glutenin subunits (HMW-GS), the Gluten quality (GQ) score and the presence of the secale block 1B3. The highest frequency of the HMW-GS 0, 7 + 9 and 5 + 10 was found among the winter wheat cultivars. The highest GQ score of 10 was found in the cultivars SK-30 and FD-92017/1. A GQ score of 9 was found in the cultivars Astella, Ilona, Velta and MV-06-95. The HMW-GS 0 and 7 +...

Relationships among Morphological Characters, Isozymes Polymorphism and DNA Variability - the Impact on Lactuca Germplasm Taxonomy

I. Doležalová, A. Lebeda, M. Dziechciarková, E. Křístková, D. Astley, C.C.M. Van De Wiel

Czech J. Genet. Plant Breed., 2003, 39(2):59-67 | DOI: 10.17221/3721-CJGPB  

Fifty one accessions of nineteen Lactuca species, the hybrid L. serriola × L. sativa and the related species Mycelis muralis were evaluated for morphological variability, esterase (EST) polymorphism, Amplified Fragment Length Polymorphism (AFLP) and relative DNA content. Sixteen Lactuca accessions were classified taxonomically on the basis of morphology, isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded allowing 82% of accessions to be distinguished. The relative DNA content, measured using flow-cytometry (DAPI staining), ranged from 2.02 pg in L. capensis to 17.96 pg...

Prof. Ing. Oldřich Chloupek, DrSc., 60 years

J. Ehrenbergerová

Czech J. Genet. Plant Breed., 2003, 39(2):68 | DOI: 10.17221/6100-CJGPB