Czech J. Genet. Plant Breed., 2003, 39(2):59-67 | DOI: 10.17221/3721-CJGPB

Relationships among Morphological Characters, Isozymes Polymorphism and DNA Variability - the Impact on Lactuca Germplasm Taxonomy

I. Doležalová, A. Lebeda, M. Dziechciarková, E. Křístková, D. Astley, C.C.M. Van De Wiel
1 Department of Botany, Faculty of Science, Palacký University, Olomouc-Holice, Czech Republic
2 Department of Gene Bank, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Praha-Ruzyně, Workplace Olomouc, Olomouc-Holice, Czech Republic
3 Department of Plant Genetics and Biotechnology, Genetic Resources Unit, Horticulture Research International, Wellesbourne, Warwick, United Kingdom
4 Business Unit Biodiversity and Identity, Plant Research International B.V., Wageningen, The Netherlands

Fifty one accessions of nineteen Lactuca species, the hybrid L. serriola × L. sativa and the related species Mycelis muralis were evaluated for morphological variability, esterase (EST) polymorphism, Amplified Fragment Length Polymorphism (AFLP) and relative DNA content. Sixteen Lactuca accessions were classified taxonomically on the basis of morphology, isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded allowing 82% of accessions to be distinguished. The relative DNA content, measured using flow-cytometry (DAPI staining), ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. The results from AFLP analysis and the relative DNA content measurement corresponded well with recent taxonomic classification of the genus Lactuca.

Keywords: AFLP; esterase; flow-cytometry; genetic resources; Lactuca spp.; morphology; PAGE electrophoresis; wild lettuce species; relative DNA content; taxomomy

Published: June 30, 2003  Show citation

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Doležalová I, Lebeda A, Dziechciarková M, Křístková E, Astley D, Van De Wiel CCM. Relationships among Morphological Characters, Isozymes Polymorphism and DNA Variability - the Impact on Lactuca Germplasm Taxonomy. Czech J. Genet. Plant Breed. 2003;39(2):59-67. doi: 10.17221/3721-CJGPB.
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