Czech Journal of Genetics and Plant Breeding, 2002 (vol. 38), issue 2

Molecular genetic mapping in apricot

J. Salava, Y. Wang, B. Krška, J. Polák, P. Komínek, R.W. Miller, W.M. Dowler, G.L. Reighard, A.G. Abbott

Czech J. Genet. Plant Breed., 2002, 38(2):65-68 | DOI: 10.17221/6113-CJGPB  

A genetic linkage map for apricot (Prunus armeniaca L.) has been constructed using amplified fragment length polymorphism (AFLP) markers in 80 BC1 individuals derived from a cross LE-3246 × Vestar. From 26 different primer combinations, a total of 248 AFLP markers were scored, of which, 40 were assigned to 8 linkage groups covering 315.8 cM of the apricot nuclear genome. The average interval between these markers was 7.7 cM. One gene (PPVres1) involved in resistance to PPV (Plum pox virus) was mapped. Two AFLP markers (EAA/MCAG8 and EAG/MCAT14) were found to be closely associated with the PPVres1 locus (4.6 cM resp....

Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

M. Baránek, M. Kadlec, J. Raddová, M. Vachůn, M. Pidra

Czech J. Genet. Plant Breed., 2002, 38(2):69-74 | DOI: 10.17221/6114-CJGPB  

The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low...

Host specialization of different populations of ergot fungus (Claviceps purpurea)

S. Pažoutová, B. Cagaš, R. Kolínská, A. Honzátko

Czech J. Genet. Plant Breed., 2002, 38(2):75-81 | DOI: 10.17221/6115-CJGPB  

In our previous study of Claviceps purpurea three populations were found: G1 on open localities, G2 from shady or wet habitats and G3 on Spartina stands of coastal salt marshes. The latter two are also chemoraces. In the Czech Republic, isolates of G1 and G2 were found. The ability of four isolates representing these populations to infect and develop sclerotia on different host species (Holcus lanatus, Helictotrichon pubescens, Phalaris arundinacea, Dactylis glomerata, Arrhenatherum elatius, Bromus inermis, Bromus erectus, Elytrigia repens, Avenella flexuosa, Lolium perenne, Poa nemoralis, Poa...

The effects of silver on microbial contamination of agar medium and on interactions between mildew and barley leaf segments with and without the mlo gene

E. Schwarzbach

Czech J. Genet. Plant Breed., 2002, 38(2):82-86 | DOI: 10.17221/6116-CJGPB  

Segments of primary leaves of several barley varieties with and without the mlo gene were placed in Petri dishes on an agar medium containing benzimidazole, mineral nutrients and 0, 0.1, 0.3, 1 and 3 ppm of AgNO3. Three Petri dishes were prepared of each concentration. The segments were uniformly inoculated with 103 conidia/cm2 of the partially mlo-virulent powdery mildew culture PV-32. Subsequently, one open Petri dish of each Ag-concentration was exposed for 1 hour to a different potential con-tamination environment: one in the laboratory (low load), one in a humid cellar close to stored vegetables (medium load) and one on the top...

Winter wheat Svitava

A. Hanišová, P. Horčička

Czech J. Genet. Plant Breed., 2002, 38(2):87-88 | DOI: 10.17221/6117-CJGPB  

Winter wheat Mladka

L. Bobková

Czech J. Genet. Plant Breed., 2002, 38(2):88-89 | DOI: 10.17221/6118-CJGPB  

Winter wheat Rheia

L. Bobková, V. Šíp, M. Škorpík

Czech J. Genet. Plant Breed., 2002, 38(2):90-91 | DOI: 10.17221/6119-CJGPB  

Winter rye Aventino

J. Čapek

Czech J. Genet. Plant Breed., 2002, 38(2):92-93 | DOI: 10.17221/6120-CJGPB  

Potato Komtesa

V. Voral

Czech J. Genet. Plant Breed., 2002, 38(2):94-95 | DOI: 10.17221/6121-CJGPB  

Prof. Ing. Jaroslav Lekeš, DrSc. (1928-2002)

I. Bareš

Czech J. Genet. Plant Breed., 2002, 38(2):96 | DOI: 10.17221/6122-CJGPB