Czech J. Genet. Plant Breed., 2002, 38(2):65-68 | DOI: 10.17221/6113-CJGPB

Molecular genetic mapping in apricot

J. Salava, Y. Wang, B. Krška, J. Polák, P. Komínek, R.W. Miller, W.M. Dowler, G.L. Reighard, A.G. Abbott
2 3 1 1 JAROSLAV SALAVA , YING WANG , BORIS KRŠKA JAROSLAV POLÁK , PETR KOMÍNEK , 5 5 2 4 ROBERT W. MILLER , WILLIAM M. DOWLER , GREGORY L. REIGHARD and ALBERT G. ABBOTT Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně,
1 Czech Republic; 2Department of Horticulture, Clemson University, Clemson, USA;
3 Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech Republic; 4Department of Biochemistry and Genetics, Clemson University, Clemson, USA; 5Department of Plant Pathology, Clemson University, Clemson, USA

A genetic linkage map for apricot (Prunus armeniaca L.) has been constructed using amplified fragment length polymorphism (AFLP) markers in 80 BC1 individuals derived from a cross LE-3246 × Vestar. From 26 different primer combinations, a total of 248 AFLP markers were scored, of which, 40 were assigned to 8 linkage groups covering 315.8 cM of the apricot nuclear genome. The average interval between these markers was 7.7 cM. One gene (PPVres1) involved in resistance to PPV (Plum pox virus) was mapped. Two AFLP markers (EAA/MCAG8 and EAG/MCAT14) were found to be closely associated with the PPVres1 locus (4.6 cM resp. 4.7 cM). These markers are being characterized and they will be studied for utilization in apricot breeding with marker-assisted selection (MAS).

Keywords: Prunus armeniaca L.; Plum pox virus; resistance; AFLP; genetic mapping

Published: June 30, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Salava J, Wang Y, Krška B, Polák J, Komínek P, Miller RW, et al.. Molecular genetic mapping in apricot. Czech J. Genet. Plant Breed. 2002;38(2):65-68. doi: 10.17221/6113-CJGPB.
Download citation

References

  1. BADENES M.L., A SÍNS M.J., CARBONELL E.A., GLÁCER G. (1996): Genetic diversity in apricot, Prunus armeniaca, aimed at improving resistance to Plum pox virus. Plant Breed., 115: 133-139. Go to original source...
  2. ELDREDGE L., BALARD R., BAIRD W.V., ABBOTT A., MORGENS P., C ALLAHAN A., SCORZA R., M ONET R. (1992): Application of RFLP analysis to genetic linkage mapping in peaches. Hort. Sci., 27: 160-163. Go to original source...
  3. GLASA M., KÚDELA O., BENEDIKOVÁ D. (2001): Comparsion of two methods of artificial inoculation used for the evaluation of apricot genotypes for resistance to Plum pox virus. In: Abstr. XIIth Int. Symp. Apricot Culture and Decline. Avignon, France: 93.
  4. GUILLET-BELLANGER I., AUDERGON J.M. (2001): Inheritance of the Stark Early Orange apricot cultivar resistance to Plum pox virus. Acta Horticult., 550: 111-115. Go to original source...
  5. HURTADO M.A., LLÁCER G., BADENES M.L., ABBOTT A.G. (2001): Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.) based on RAPD and AFLP markers. In: Abstr. XIIth Int. Symp. Apricot Culture and Decline. Avignon, France: 56.
  6. KOSAMBI D.D. (1944): The estimation of map distance from recombination values. Ann. Eugen., 12: 172-175. Go to original source...
  7. LAMBERT P., HAGEN L.S., AUDERGON J.M., ARUS P. (2001): Evaluation of a mapping strategy based on syntheny: using the Prunus reference map for apricot. In: Abstr. XIIth Int. Symp. Apricot Culture and Decline. Avignon, France: 55.
  8. LINCOLN S., DALY M., LANDER E.S. (1992): Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report. 3rd ed. Whitehouse Technical Institute, Cambridge, Mass.
  9. LU Z.-X., SOSINSKI B., REIGHARD G.L., BAIRD W.M., ABBOTT A.G. (1998): Construction of a genetic linkage map and identification of markers for resistance to root-knot nematodes in peach rootstocks. Genome, 41: 199-207. Go to original source...
  10. MOUSTAFA T.A., BADENES M.L., MARTINEZ-CALVO J., LLÁCER G. (2001): Studies on plum pox (Sharka) resistance in apricot. Acta Hort., 550: 117-120. Go to original source...
  11. POLÁK J., KRŠKA B., KOMÍNEK P., SASKOVÁ H., SALAVA J. (2001): Preliminary studies on the inheritance of resistance to Plum pox virus (PPV) in apricots. In: Proc. Middle Eur. Meet. Plum Pox Virus. Pitesti, Romania: (in press).
  12. PONCAROVÁ Z., KOMÍNEK P. (1998): Restriction fragment length polymorphism differentiation of plum pox virus isolates. Acta Virol., 42: 268-269.
  13. SALAVA J., WANG Y., KRŠKA B., POLÁK J., KOMÍNEK P., MILLER W., DOWLER W.M., R EIGHARD G.L., ABBOTT A.G. (2002): Identification of molecular markers linked to resistance of apricot (Prunus armeniaca L.) to plum pox virus. J. Plant Dis. Protect., 109: 64-67.
  14. VOS P., HOGERS R., BLEEKER M., RIJANS M., VAN DE LEE T., HORNES M., FRIJTERS A., POT J., PELEMAN J., KUIPER M., ZABEAU M. (1995): AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4407-4414. Go to original source... Go to PubMed...
  15. WANG Y., REIGHARD G.L., SCORZA R., ABBOTT A.G. (2001): Genetic and physical mapping of the evergreen gene. In: Plant and Animal Genome, IX.: w27 (http://www.intlpag.org/pag/9/abstracts/W27_03html)(abstract).
  16. WANG Y.-H., THOMAS C.E., DEAN R.A. (1997): A genetic map of melon (Cucumis melo L.) based on amplified fragment lenght polymorphism (AFLP) markers. Theor. Appl. Genet., 95: 791-798. Go to original source...
  17. ZABEAU M., VOS P. (1993): Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application number: 92402629.7, Publ. No. EP 0534858.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.