Czech J. Genet. Plant Breed., 2019, 55(2):76-82 | DOI: 10.17221/57/2018-CJGPB

Identification of manganese-responsive microRNAs in Arabidopsis by small RNA sequencingOriginal Paper

Jian Gong1,*, Dong Li3,5, Hao Li4,5, Huakun Zhou2, Jin Xu*,5
1 Pharmaceutical and Biological Engineering Department, Zibo Vocational Institute, Zibo, P.R. China
2 Northwest Institute of Plateau Biology, Key Laboratory of Restoration Ecology of Cold Area in Qinghai Province, Chinese Academy of Sciences, Xining, P.R. China
3 College of Horticulture, Shanxi Agricultural University, Taigu, P.R. China
4 College of Forestry, Shanxi Agricultural University, Taigu, P.R. China
5 CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, P.R. China

Manganese (Mn) is an important micronutrient for growth and development in plants, however, excess Mn is harmful by disrupting photosynthesis system and inducing oxidative damage in leaves. MicroRNAs (miRNAs) play key roles in regulating Mn toxicity tolerance in plants. Here, we identified Mn toxicity-responsive miRNAs in Arabidopsis by using small RNA sequencing. Eighteen differentially expressed miRNAs were identified in Arabidopsis thaliana seedlings in response to Mn toxicity. These differentially expressed miRNAs are involved in regulating nutrition homeostasis, transport, stress response, and developmental processes. Our results indicated that these miRNAs play a key role in Mn toxicity response in plants.

Keywords: Arabidopsis thaliana; high-throughput sequencing; manganese toxicity; miRNA

Published: June 30, 2019  Show citation

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Gong J, Li D, Li H, Zhou H, Xu J. Identification of manganese-responsive microRNAs in Arabidopsis by small RNA sequencing. Czech J. Genet. Plant Breed. 2019;55(2):76-82. doi: 10.17221/57/2018-CJGPB.
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