Czech J. Genet. Plant Breed., 2015, 51(2):43-49 | DOI: 10.17221/2/2015-CJGPB

Identification of a leaf rust resistance gene in the Chinese wheat line LB0288Original Paper

Aiyong QI, Xing LI, Lingzhi SHI, Daqun LIU, Zaifeng LI
College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China

Leaf rust (LR), caused by Puccinia triticina (Pt), is worldwide one of the most spread diseases in common wheat (Triticum aestivum L.). With the objective of identifying and mapping new genes for resistance to leaf rust, F1 and F2 plants from a cross between the resistant cultivar LB0288 and the susceptible cultivar Thatcher were inoculated with the Chinese P. triticina pathotype THTT in the greenhouse. A set of 1273 simple sequence repeat (SSR) primer pairs were used to test the parents and the resistant and susceptible bulks. The results indicated, that LB0288 carried a single dominant resistance gene LrLB88, closely linked to the CAPS marker of Lr1 (WR003) and the SSR marker Xbarc144, with genetic distances of 0 cM and 5.3 cM, respectively. Based on the chromosomal location and seedling reactions, it can be concluded that LrLB88 might be a new leaf rust resistance gene. These markers can be useful for marker assisted selection in breeding of leaf rust resistant wheat cultivars.

Keywords: leaf rust resistance gene; molecular mapping; wheat

Published: June 30, 2015  Show citation

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Aiyong Q, Xing L, SHI L, LIU D, Zaifeng L. Identification of a leaf rust resistance gene in the Chinese wheat line LB0288. Czech J. Genet. Plant Breed. 2015;51(2):43-49. doi: 10.17221/2/2015-CJGPB.
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