Czech Journal of Genetics and Plant Breeding, 2015 (vol. 51), issue 2

Identification of a leaf rust resistance gene in the Chinese wheat line LB0288Original Paper

Aiyong QI, Xing LI, Lingzhi SHI, Daqun LIU, Zaifeng LI

Czech J. Genet. Plant Breed., 2015, 51(2):43-49 | DOI: 10.17221/2/2015-CJGPB  

Leaf rust (LR), caused by Puccinia triticina (Pt), is worldwide one of the most spread diseases in common wheat (Triticum aestivum L.). With the objective of identifying and mapping new genes for resistance to leaf rust, F₁ and F₂ plants from a cross between the resistant cultivar LB0288 and the susceptible cultivar Thatcher were inoculated with the Chinese P. triticina pathotype THTT in the greenhouse. A set of 1273 simple sequence repeat (SSR) primer pairs were used to test the parents and the resistant and susceptible bulks. The results indicated, that LB0288 carried a single dominant...

Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markersOriginal Paper

Imane MEDOUKALI, Inès BELLIL, Douadi KHELIFI

Czech J. Genet. Plant Breed., 2015, 51(2):50-61 | DOI: 10.17221/10/2015-CJGPB  

Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard's similarity coefficient ranged between...

The selection and validation of a marker set for the differentiation of onion cultivars from the Czech RepublicOriginal Paper

Katarína MITROVÁ, Pavel SVOBODA, Jaroslava OVESNÁ

Czech J. Genet. Plant Breed., 2015, 51(2):62-67 | DOI: 10.17221/16/2015-CJGPB  

The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity...

Evaluation of selected Cucumis sativus accessions for resistance to Pseudoperonospora cubensis in EgyptOriginal Paper

Elmahdy Ibrahim METWALLY, Mohamed Tawfik RAKHA

Czech J. Genet. Plant Breed., 2015, 51(2):68-74 | DOI: 10.17221/12/2015-CJGPB  

Downy mildew [Pseudoperonospora cubensis (Berk. & Curt.) Rostov] is a major destructive disease of cucumber (Cucumis sativus L.) worldwide. Resistant cultivars were recently introduced into Egypt, but yield losses were high if no fungicides were used. The objective of this study was to identify sources of resistance to downy mildew among Plant Introduction (PI) cucumber accessions from the U.S. National Plant Germplasm System. We evaluated 133 such accessions for downy mildew resistance under natural field epidemics during the summer 2013 at El-Beheira Governorate, Egypt. Mean ratings for downy mildew leaf damage ranged from 1...

Six-row winter barley LancelotNew Varieties

Pavel MAŘÍK, Jana CHRPOVÁ, Ilja Tom PRÁŠIL, Tibor SEDLÁČEK

Czech J. Genet. Plant Breed., 2015, 51(2):75-77 | DOI: 10.17221/61/2015-CJGPB  

The Lancelot variety is a late to semi-late six-row feeding winter barley. It was developed at the Breeding Station, Lužany, SELGEN a.s. and registered in the Czech Republic in 2013. Lancelot has very good resistance to winter stresses in combination with resistance to BaMMV/BaYMV (Barley mild mosaic virus/Barley yellow mosaic virus) based on the gene rym4.