Czech J. Genet. Plant Breed., 2014, 50(4):285-287 | DOI: 10.17221/70/2014-CJGPB

Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomatoShort Communication

Mirosława STANIASZEK1, Wojciech SZCZECHURA1, Waldemar MARCZEWSKI2
1 Department of Genetics, Breeding and Biotechnology of Vegetable Plants, Research Institute of Horticulture, Skierniewice, Poland
2 Plant Breeding and Acclimatization Institute, National Research Institute, Młochów, Poland

Fusarium oxysporum Schlecht. f.sp. radicis-lycopersici Jarvis & Schoemaker (FORL) is a saprophytic fungus, responsible for the fusarium crown and root rot disease in tomato (Solanum lycopersicum L.). This is one of the most destructive pathogens of this species. A new cleaved amplified polymorphic sequence (CAPS) marker C2-25 was developed for the detection of the dominant gene Frl, which confers tomato resistance to FORL. C2-25 was amplified from a conserved ortholog set II (COSII) sequence C2_At2g38025. The XapI-derived restriction product of 700 bp was informative for the identification of FORL resistant tomato genotypes and can be used as a diagnostic marker in tomato breeding programmes and hybrid seed production.

Keywords: CAPS marker; fusarium crown and root rot; marker assisted selection (MAS); Solanum lycopersicum L.

Published: December 31, 2014  Show citation

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STANIASZEK M, SZCZECHURA W, MARCZEWSKI W. Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato. Czech J. Genet. Plant Breed. 2014;50(4):285-287. doi: 10.17221/70/2014-CJGPB.
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