Czech J. Genet. Plant Breed., 2014, 50(2):130-134 | DOI: 10.17221/231/2013-CJGPB

Hybridization of cultivated lentil Lens culinaris Medik. and wild species Lens tomentosus LadizinskyOriginal Paper

Galina SUVOROVA
All-Russia Research Institute of Legumes and Groat Crops, Orel, Russia

Cultivated lentil L. culinaris was crossed to the wild species L. tomentosus ILWL90 and ILWL120. An ovule rescue technique was used to overcome interspecific incompatibility. Out of 296 hybrid ovules being planted in vitro 27 explants began to grow and three hybrids were recovered. A hybrid between L. culinaris and L. tomentosus accession ILWL90 was obtained by means of ovule recovery only. F1 plant and next generations of the hybrid were either sterile or partly fertile. Hybridization with L. tomentosus accession ILWL120 was achieved by ovule culture as well as in a usual way i.e. without ovule culture. Seed progenies of these hybrids were fertile in both cases. Breeding lines recombinant in flower, seed coat and cotyledon coloring were developed as a result of multiple regular selection for highly productive plants in F2-F7 (L. culinaris × L. tomentosus ILWL120).

Keywords: interspecific hybridization; Lens culinaris; Lens tomentosus; lentil; ovule rescue

Published: June 30, 2014  Show citation

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SUVOROVA G. Hybridization of cultivated lentil Lens culinaris Medik. and wild species Lens tomentosus Ladizinsky. Czech J. Genet. Plant Breed. 2014;50(2):130-134. doi: 10.17221/231/2013-CJGPB.
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    References

    1. Cubero J.I., Perez De La Vega M., Frantini R. (2009): Origin, phylogeny, domestication and spread. In: Erskine W. et al. (eds): The Lentil. Botany, Production and Uses. CABI, Wallingford, 13-33. Go to original source...
    2. Doyle J., Doyle L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Go to original source...
    3. Duran Y., Perez De La Vega M. (2004): Assessment of genetic variation and species relationships in a collection of Lens using RAPD and ISSR. Spanish Journal of Agricultural Research, 2: 538-544. Go to original source...
    4. Fiala J.V., Tullu A., Banniza S., Seguin-Swartz G., Vandenberg A. (2009): Interspecific transfer of resistance to anthracnose in lentil (Lens culinaris Medik.). Crop Science, 49: 825-830. Go to original source...
    5. Fratini R., Ruiz M.L. (2003): A rooting procedure for lentil (Lens culinaris Medik.) and other hypogeous legumes (pea, chickpea and Lathyrus) based on explant polarity. Plant Cell Reports, 21: 726-732. Go to original source... Go to PubMed...
    6. Fratini R., Ruiz M.L. (2006): Interspecific hybridization in the genus Lens applying in vitro embryo rescue. Euphytica, 150: 271-280. Go to original source...
    7. Galasso I. (2003): Distribution of highly repeated DNA sequences in species of the genus Lens Miller. Genome, 46: 1118-1124. Go to original source... Go to PubMed...
    8. Ladizinsky G. (1993): Wild lentils. Critical Reviews in Plant Sciences, 12: 169-184. Go to original source...
    9. Ladizinsky G., Cohen D., Meuhlbauer F.J. (1985): Hybridization in the genus Lens by means of embryo culture. Theoretical and Applied Genetics, 70: 97-101. Go to original source... Go to PubMed...
    10. Sonnante G., Galasso I., Pignone D. (2003): ITS Sequence analysis and phylogenetic inference in the genus Lens Mill. Annals of Botany, 91: 49-54. Go to original source... Go to PubMed...
    11. Suvorova G., Kornienko N. (2011) Genetic relationships among wild Lens Mill. species revealed by SDS-PAGE. Field and Vegetable Crop Research, 48: 31-36. Go to original source...
    12. Tullu A., Diederichsen A., Suvorova G., Vandenberg A. (2011): Genetic and genomic resources of lentil: status, use and prospects. Plant Genetic Resources: Characterization and Utilization, 9: 19-21. Go to original source...
    13. Tullu A., Bett A., Banniza S., Vandenberg A. (2013): Widening of genetic base of cultivated lentil through hybridization of Lens culinaris 'Eston' and L. ervoides accession IG 72815. Canadian Journal of Plant Science, 93: 1-11. Go to original source...
    14. van Oss H., Aron Y., Ladizinsky G. (1997): Chloroplast DNA variation and evolution in the genus Lens Mill. Theoretical and Applied Genetics, 94: 452-457. Go to original source...

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