Czech J. Genet. Plant Breed., 2010, 46(10):S96-S99 | DOI: 10.17221/2448-CJGPB

Evaluation of winter wheat collection in terms of HMW- and LMW-glutenin subunits

J. Bradová, L. Štočková
Crop Research Institute, 161 06 Prague-Ruzyně, Czech Republic,

The composition of high molecular weight (HMW-GS) and low molecular weight (LMW-GS) glutenin subunits was examined in a collection of 86 Czech registered winter wheat varieties. These proteins were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. An inter-varietal polymorphism of the HMW and LMW glutenin subunits was detected. Twenty-one different patterns for HMW were identified, and eighteen for the LMW-glutenins. The different alleles encoded at the six glutenin loci were determined. Three, six, and four alleles were observed, respectively at the Glu-A1, Glu-B1, and Glu-D1 loci (encoding high HMW-GS). Three, eight, and three alleles of LMW-GS were found, respectively, at the Glu-A3, Glu- B3, and Glu-D3 loci. The evaluated varieties were split into four categories of baking quality, and these variety groups were analyzed for the presence of different HMW-GS and LMW-GS alleles. While the alleles Glu-B1c (7+9), and Glu-D1d (5+10) were detected exclusively in bread wheat varieties, the alleles Glu-B1d (6+8), Glu-D1a (2+12), and Glu-A3e/f only occurred in those varieties that are not suitable for bread-making.

Keywords: characterization; electrophoresis; genetic diversity; glutenin subunits; Triticum aestivum L.

Published: December 31, 2010  Show citation

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Bradová J, Štočková L. Evaluation of winter wheat collection in terms of HMW- and LMW-glutenin subunits. Czech J. Genet. Plant Breed. 2010;46(Special Issue):S96-99. doi: 10.17221/2448-CJGPB.
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