Czech J. Genet. Plant Breed., 2007, 43(4):144-148 | DOI: 10.17221/1900-CJGPB

Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding

Marie Špunarová, Milan Pouch, Jaroslav Špunar
Agrotest fyto, Ltd., Kroměříž, Czech Republic

The success in hybridization of two-rowed spring barley genotypes of various origins was verified using the codominant SSR (Simple Sequence Repeat) marker HVWAXY which is located in the coding sequence of the waxy gene. The primer pair F 5' AAG ACG TGG TGT TCG TGT G 3' and R 5' ATG GTT CCA GGG GTA AGT TC 3' generated the PCR product of approximately 200 bp in the varieties/breeding lines Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2439, KM 2629, KM-H-1320, whereas the product of about 250 bp was amplified in the lines KM 2416 and Br 7571h33. The parental genotypes possessing different alleles of the marker were crossed and the segregating lines of the F3 generation were characterized employing molecular methods (marker-assisted selection). This prescreening enabled to work with a lower number of individuals (selfed individuals were excluded) at the initial stages of the breeding process leading to the acceleration of selection as a particular phase of breeding.

Keywords: barley; Hordeum vulgare L.; hybridization; marker-assisted selection; SSR marker

Published: December 31, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Špunarová M, Pouch M, Špunar J. Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding. Czech J. Genet. Plant Breed. 2007;43(4):144-148. doi: 10.17221/1900-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Becker J., Huen M. (1995): Barley microsatellites: allele variation and mapping. Plant Molecular Biology, 27: 835-845. Go to original source... Go to PubMed...
    2. Czembor J.H., Talbert L.E. (1997): Evaluation of STSPCR and RFLPs as molecular markers for the Mla locus conferring powdery mildew resistance in barley. Plant Breeding and Seed Science, 41: 1-14.
    3. Han F., Romagosa I., Ullrich S.E., Jones B.L., Hayes P.M., Wesenberg D.M. (1997): Molecular markerassisted selection for malting quality traits in barley. Molecular Breeding, 3: 427-437. Go to original source...
    4. Lee S.J., Penner G.A. (1997): The conversion of RFLP markers to allele specific amplicons linked to QTLs governing malting quality in barley. Molecular Breeding, 3: 457-462. Go to original source...
    5. Malysheva L.V., Roder M.S. (2006): Haplotype diversity in the endosperm specific beta-amylase gene Bmy1 of cultivated barley (Hordeum vulgare L.). Molecular Breeding, 18: 143-156. Go to original source...
    6. Malysheva L.V., Ganal M.W., Roder M.S. (2004): Evaluation of cultivated barley (Hordeum vulgare) germplasm for the presence of thermostable alleles of beta-amylase. Plant Breeding, 123: 128-131. Go to original source...
    7. Manninen O.M., Turpeinen T., Nissila E. (1997): Identification of RAPD markers closely linked to the mlo locus in barley. Plant Breeding, 116: 461-464. Go to original source...
    8. Ovesná J., Kučera L., Poláková K., Vaculová K., Milotová J. (2004): Assessment of diversity within Czech spring barley germplasm as revealed by PCR diagnostic marker, SSR, AFLP and DNA assays. Czech Journal of Genetics and Plant Breeding (Special Issue), 40: 24.
    9. Paltridge N.G., Collins N.C., Bendahmane A., Symons R.H. (1998): Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease. Theoretical and Applied Genetics, 96: 1170-1177. Go to original source...
    10. Paris M., Jones M.G.K., Eglinton J.K. (2002): Genotyping single nucleotide polymorphisms for selection of barley beta-amylase alleles. Plant Molecular Biology Reporter, 20: 149-159. Go to original source...
    11. Poulsen D.M.E., Johnston R.P., Henry R.G., Irwin J.A.G. (1996): Molecular markers: their potential use to barley breeding in north-east Australia. In: VII IBGS Proc., Vol. 1, Saskatoon, 289-291.
    12. Schwarz G., Michalek W., Mohler V., Wenzel G., Jahoor A. (1999): Chromosome landing at the Mla locus in barley (Hordeum vulgare L.) by means of highresolution mapping with AFLP markers. Theoretical and Applied Genetics, 98: 521-530. Go to original source...
    13. Steffenson B.J., Smith K.P. (2006): Breeding barley for multiple disease resistance in the upper midwest region of the USA. Czech Journal of Genetics and Plant Breeding, 42: 79-85. Go to original source...
    14. Šíp V., Chrpová J., Vacke J., Ovesná J. (2004): Possibility of exploiting the Yd2 resistance to BYDV in spring barley breeding. Plant Breeding, 123: 24-29. Go to original source...
    15. Špunarová M., Kraus I. (2000): Evaluation of hybridization success in spring barley by means of a genetic marker. Czech Journal of Genetics and Plant Breeding, 36: 5-9.
    16. Varshney R.K., Hoisington D.A., Tyagi A.K. (2006): Advances in cereal genomics and applications in crop breeding. Trends in Biotechnology, 24: 490-499. Go to original source... Go to PubMed...
    17. Weising W.H., Wolff K., Meyer W. (1995): DNA Fingerprinting in Plants and Fungi. CRC Press, Boca Raton, 171-191.
    18. William M., Dorocizc I., Kasha K.J. (1997): Use of microsatellite DNA to distinguish malting and nonmalting barley cultivars. Journal of the American Society of Brewing Chemists, 55: 107-111. Go to original source...
    19. Williams J.G.K., Kubelik A.R., Liva K. J., Rafalski J.A., Tingey S.V. (1990): DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content