Czech J. Genet. Plant Breed., 2005, 41(2):51-63 | DOI: 10.17221/3672-CJGPB

Simulation and Assessment of Possible Environmental Risks Associated with Release of Genetically Modified Peas (Pisum sativum L.) into Environment in Central Europe

R. Dostálová1, M. Seidenglanz2, M. Griga3
1 Department of Grain Legumes
2 Plant Protection Department
3 Plant Biotechnology Department, AGRITEC, Ltd., ©umperk, Czech Republic

Environmental risks connected with the release of genetically modified peas into the environment were studied in simulated field experiments. They included (1) an assessment of pollen transfer and the rate of natural outcrossing between commercial peas (Pisum sativum), (2) the incidence and behaviour of insects visiting pea flowers and their role as potential pollinators, and (3) the inventory of other insect taxa in pea crop (not related directly to pea reproduction organs). Field trials were established (2001-2003) with two non-GM pea cultivars differing in flower colour, seed coat colour and whole plant habit that were grown in close proximity. Cv. Zekon with recessive traits served as a trap variety, cv. Arvika with dominant traits as a pollen donor. The seeds of the trap variety were completely harvested and sown each successive year (2002, 2003) to monitor the incidence of dominant traits in F1 generation. In the case of outcrossing, dominant traits would occur already in F1 generation. However, the occurrence of plants with dominant traits in the progeny of the trap variety was not recorded in any case during the whole experimentation period (ca 40 thousand F1 plants screened each year). Based on the obtained data we assume that the probability of outcrossing in recent commercial peas is extremely low (zero in our experiments). Among the species visiting regularly pea flower buds/flowers (pests, pollinators) the most frequent were pea weevil (Bruchus pisorum), pea aphid (Acyrthosiphon pisum), pea thrips (Kakothrips robustus), honey-bee (Apis mellifera), bumble-bees (Bombus sp.). Bruchus pisorum is a possible candidate for pollen transfer in unopened pea flowers. A list of insect taxa occurring in pea fields was created on the basis of the four-year monitoring (2001-2004).

Keywords: pea; Pisum sativum; transgenic plants; insect pollinators; outcrossing; environmental risks

Published: June 30, 2005  Show citation

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Dostálová R, Seidenglanz M, Griga M. Simulation and Assessment of Possible Environmental Risks Associated with Release of Genetically Modified Peas (Pisum sativum L.) into Environment in Central Europe. Czech J. Genet. Plant Breed. 2005;41(2):51-63. doi: 10.17221/3672-CJGPB.
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    References

    1. A B.A., O'K L.E. (1984): Effect of pollen source on oogenesis in the pea weevil, Bruchus pisorum L. (Coleoptera: Bruchidae). Protection Ecology, 6: 257-266.
    2. B S.J., G P.S., M P.M., D D.R. (1997): A simple system for pea transformation. Plant Cell Reports, 16: 513-519. Go to original source... Go to PubMed...
    3. B -Z ' N., Z S. (1973): Species relationships in the genus Pisum L. Israel Journal of Botany, 22: 73-91.
    4. B V.S., B V.A. (2000): A study of potential ability for cross-pollination in pea originating from different parts of the world. Pisum Genetics, 32: 16-17.
    5. C P. (1997): Biotechnology applied to grain legumes. Field Crops Research, 53: 83-97. Go to original source...
    6. C S.L. (1992): On the function of pea flower feeding by Bruchus pisorum. Entomologia Experimentalis et Applicata, 63: 115-121. Go to original source...
    7. C S.L., L L.A., M F.J. (1988): More on the pea's nectaries and insect visitors. The Pisum Newsle er, 20: 3-4.
    8. C A.J., G T.R., N J.-P. (2003): The release of genetically modified crops into the environment. Part II. Overview of ecological risk assessment. The Plant Journal, 33: 19-46. Go to original source... Go to PubMed...
    9. C C., E A. (1993): Embryology of ovule abortion in reciprocal crosses between diploids and tetraploids in Pisum sativum and P. fulvum. Journal of Genetics and Breeding, 47: 157-162.
    10. C D.C. (1938): Embryology of Pisum sativum. Botanical Gaze e, 100: 123-132. Go to original source...
    11. D D.R. (1993): The pea crop. In: C R., D D.R. (eds): Peas: Genetics, Molecular Biology and Biotechnology. CAB International, Wallingford, 1-12.
    12. D R., S M., G M. (2004): Monitoring of environmental risks of genetically modified pea (Pisum sativum L.) in CR: model solution. In: O J., K L. (eds): Problems of biological safety, GMOs and international involvement of the Czech Republic. VÚRV Praha-Ruzyně, 25-34. (in Czech)
    13. D P., O S.J. (2000): Efficient intergeneric protoplast fusion between pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.). Journal of Experimental Botany, 51: 1237-1242. Go to original source...
    14. E -R M., T J.D. (1998): Inheritance of potential race non-specific resistance derived from Pisum abyssinicum for the control of pea bacterial blight (Pseudomonas syringae pv. pisi). In: Proceedings 3rd European Conference on Grain Legumes - Opportunities for High Quality, Healthy and Added-Value Crops to Meet European Demands. Valladolid, Spain, 234.
    15. E A., C C., V G. (1991): Karyotype studies on Pisum fulvum and Pisum sativum using a chromosome image analysis system. Genome, 34: 105-108. Go to original source...
    16. E A., C C., D M T., E R., M L.M. (1996): Chromosome reconstructions in Pisum sativum through interspecific hybridization with P. fulvum. Journal of Genetics and Breeding, 50: 309-313.
    17. G B.V., K K.K. (1986): Pea. In: E D.A., S W.R., A, P.V. (eds): Handbook of Plant Cell Culture, Vol. 4, Techniques and Applications. Macmillan Publ. Comp., New York, 370-418.
    18. G L.B., M M.R.C., M P.E. (1991): Estimates of natural outcrossing in peas in Brasilia - DF. Horticultura Brasileira, 9: 82-83 (in Portuguese).
    19. G A.A., Y I.I. (1982): Prospects for wide hybridization in pea as related to the problem of sources of resistance to diseases. Trudy po Prikladnoj Botanike, Genetike i Selekcii, 71: 75-78 (in Russian).
    20. G L.I. (1928): The peas of Afghanistan. Trudy po Prikladnoj Botanike, Genetike i Selekcii, 19: 517-522 (in Russian).
    21. G J.E., C P.A., M A A.E., F T.J. (1995): Transformation of peas (Pisum sativum L.) using immature cotyledons. Plant Cell Reports, 15: 254-258. Go to original source... Go to PubMed...
    22. G M., K G., K N., I -S M. (2001): Biotechnology. In: H C. (ed.): Carbohydrates in Grain Legume Seeds: Improving Nutritional Quality and Agronomic Characteristics. CAB International, Wallingford Oxon, U.K., 145-207. Go to original source...
    23. G E.T. (1969): Crosses between Pisum and closely related genera. The Pisum Newsle er, 1: 7.
    24. G E.T. (1980): Field pea. In: F W.R., H H.H. (eds.): Hybridization of Crop Plants. American Society of Agronomy, Madison, Wisconsin, 347-356.
    25. G E.T., W B. (1975): An embryological study of a Pisum sativum × Vicia faba cross. Euphytica, 24: 277-284. Go to original source...
    26. H S.C. (1948): Inheritance of immunity to mildew in Peruvian forms of Pisum sativum. Heredity, 2: 263-269. Go to original source... Go to PubMed...
    27. H J., B P. (1991): Bruchus pisorum L. (Coleoptera; Bruchidae) control by a knockdown pyrethroid in field peas. Crop Protection, 10: 53-56. Go to original source...
    28. J C. (2003): Preview: Global status of commercialized transgenic crops: 2003. ISAAA Briefs No. 30. ISAAA, Ithaca, N.Y.
    29. J A.L., J I.E., B S.J., B I., M A.J. (1998): Specificity of resistance to pea seed-borne mosaic potyvirus in transgenic peas expressing the viral replicase (Nib) gene. Journal of General Virology, 79: 3129-3137. Go to original source... Go to PubMed...
    30. L W.E. (1983): Cross-fertilization in peas. The Pisum Newsle er, 15: 40.
    31. L W.E. (1984): Cross-fertilization in peas under different ecological conditions. The Pisum Newsletter, 16: 38-40.
    32. L W.E. (1985): The peas' nectaries and insect visitors. The Pisum Newsle er, 17: 47-49.
    33. M R.L., S H.E., B K.S., C M.J., A E., H T.J.V. (2000): Bean α-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions. Proceedings of the National Academy of Sciences of the USA, 97: 3820-3825. Go to original source... Go to PubMed...
    34. O S.J., B A., M P., A G., M F., P C., J L. (2004): Overcoming hybridization barriers between pea and some of its wild relatives. Euphytica, 137: 353-359. Go to original source...
    35. O H.A. (1963): Occurence of allogamy in pea. Revista de Olericultura, 3: 83-90 (in Portuguese).
    36. P H.R. (1981): Trophic relations and ecological status of the adults of Bruchus pisorum L. and allied field species of Bruchidae (Coleoptera). In: L V. (ed.): The Ecology of Bruchidae A acking Legumes (Pulses). Junk, Dordrecht, the Netherlands, 125-129. Go to original source...
    37. P H.R. (1987): Ecological status of host range and polymorphism in Bruchidae. In: D E., N S. (eds): Proceedings 4th International Working Conference on Stored Product Protection. Tel Aviv, Agricultural Research Organization, Bet Dagan, Israel, 506-516.
    38. P G.R., H R.J. (1982): Pollen and sexual maturation in the pea weevil (Coleoptera; Bruchidae). Annals of the Entomological Society of America, 75: 439-443. Go to original source...
    39. P P.L., V A., M J.D. (2002): Field assessment of outcrossing from transgenic pea (Pisum sativum L.) plants. Transgenic Research, 11: 515-519. Go to original source... Go to PubMed...
    40. P -K J., E T., E P. (1990): Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens-mediated gene transfer. Theoretical and Applied Genetics, 80: 246-252. Go to original source... Go to PubMed...
    41. P -K J., E T., E P. (1992): Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants. Theoretical and Applied Genetics, 84: 443-450. Go to original source... Go to PubMed...
    42. R S., O M., S F., H O., H H.M., O J., K L., K F., Ř K., D R., S M., T E., G M. (2004): Transgenic plant products and their introduction into the environment and crop protection systems, a risk assessment. In: N J.-P., A A., S W.J. (eds): Genomics for Biosafety in Plant Biotechnology. IOS Press, Amsterdam, 173-184.
    43. S H., S A., W -R T., S D., H T. (1993): Transformation and regeneration of two cultivars of pea (Pisum sativum L.). Plant Physiology, 101: 751-757. Go to original source... Go to PubMed...
    44. S H.E., G S., M A., T L.M., C S., H D.C., C M.J., S D., H T.J.V. (1995): Bean alfa-amylase inhibitor confers resistance to the pea weevil (Bruchus pisorum) in transgenic peas (Pisum sativum). Plant Physiology, 107: 1233-1239. Go to original source... Go to PubMed...
    45. S R.E., S H.E., P J.J., T L.M., M L.L., H T.J.V., C M.J. (1994): Transgenic pea seeds expressing the α-amylase inhibitor of the common bean are resistant to bruchid beetles. Bio/Technology, 12: 793-796. Go to original source...
    46. S J. (1979): Interspecific hybridization in the grain legumes - a review. Economic Botany, 33: 329-337. Go to original source...
    47. © L., O V., G M. (2002): Genetic modification of pea (Pisum sativum L.) with the use of multiple shoot culture and in vivo regeneration system. In: G M., O V., H J. (eds): Utilization of molecular markers in plant biology, plant breeding and plant genetic resources conservation. AGRITEC, ©umperk, 207-214 (in Czech).
    48. © L., O V., H J., G M. (2004): Agrobacterium-mediated pea transformation by in vitro and in vivo approaches. In: Proceedings 5th European Conference on Grain Legumes - Legumes for the Benefit of Agriculture, Nutrition and the Environment: Their Genomics, Their Products, and Their Improvement. Dijon, France, 198.
    49. © L., S P., G M., O V. (2005): Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo. Biologia Plantarum, 49: 361-370. Go to original source...
    50. T -V G.M., P -J M.D., C P.A., R A.C., G J.E. (2001): Partial resistance of transgenic peas to alfalfa mosaic virus under greenhouse and field conditions. Crop Science, 41: 846-853. Go to original source...
    51. W J.M. (1998): Possible role for wild genotypes of Pisum spp. to enhance ascochyta blight resistance in pea. Australian Journal of Experimental Agriculture, 38: 469-479. Go to original source...
    52. Z M. (1980): Intraovarian and in vitro pollination. International Review of Cytology, Suppl. 11B: 137-156.

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