Czech J. Genet. Plant Breed., 2002, 38(1):50-54 | DOI: 10.17221/6110-CJGPB

Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

V. Kučera, M. Vyvadilová, M. Klíma
Research Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech Republic

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

Keywords: winter oilseed rape; doubled haploid; homozygous line; breeding

Published: March 31, 2002  Show citation

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Kučera V, Vyvadilová M, Klíma M. Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding. Czech J. Genet. Plant Breed. 2002;38(1):50-54. doi: 10.17221/6110-CJGPB.
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