Czech Journal of Genetics and Plant Breeding, 2020 (vol. 56), issue 4
Index of Volume 56 (2020) - Authors IndexIndex
editors
Czech J. Genet. Plant Breed., 2020, 56(4):I-IV | DOI: 10.17221/12991-CJGPB 
Potentials to breed for improved fibre digestibility in temperate Czech maize (Zea mays L.) germplasmOriginal Paper
Manfred Schönleben, Joachim Mentschel, Luboš Střelec
Czech J. Genet. Plant Breed., 2020, 56(4):133-139 | DOI: 10.17221/11/2020-CJGPB
Cell wall digestibility is an important quality trait of modern silage maize cultivars. The symbiotic relationship between microbes and ruminant livestock enables the efficient upcycling of otherwise for human consumption unsuitable rumen digestible fibre or cell wall components into highly nutritious milk and meat. Before entering the Czech National List of Plant Varieties, new silage maize germplasm is extensively tested for different cell wall digestibility parameters. Recently published, the undigestible neutral detergent fibre (uNDF) cell wall digestibility approach promises even greater practical relevance. The aim of our study was, therefore,...
Phenotypic traits for wild red clover seed yield under drought conditionsOriginal Paper
Giedrius Petrauskas, Eglė Norkevičienė, Vaclovas Stukonis, Vilma Kemešytė
Czech J. Genet. Plant Breed., 2020, 56(4):140-149 | DOI: 10.17221/111/2019-CJGPB
Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover...
Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther cultureOriginal Paper
Osama Zuhair Kanbar, Csaba Lantos, Paul Karumba Chege, Erzsebet Kiss, Janos Pauk
Czech J. Genet. Plant Breed., 2020, 56(4):150-158 | DOI: 10.17221/113/2019-CJGPB
We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25-87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending...
Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem cultureOriginal Paper
Josef Patzak, Alena Henychová, Petr Svoboda, Ivana Malířová
Czech J. Genet. Plant Breed., 2020, 56(4):159-164 | DOI: 10.17221/27/2020-CJGPB
In vitro meristem cultures have been used for the production of hop (Humulus lupulus L.) virus-free rootstocks worldwide, because multipropagation is considered to preserve the genetic stability of the produced plantlet. Nevertheless, in vitro tissue cultures can cause genetic and epigenetic changes. Therefore, we studied the genetic and epigenetic variability of Saaz Osvald's clones, Sládek and Premiant cultivars on the DNA methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro propagated plants, acclimatised glasshouse rootstocks as well as derived mericlones and control plants under...
Molecular screening of domestic apple cultivars for scab resistance genes in GreeceShort Communication
Lefkothea Karapetsi, Irini Nianiou-Obeidat, Antonios Zambounis, Maslin Osathanunkul, Panagiotis Madesis
Czech J. Genet. Plant Breed., 2020, 56(4):165-169 | DOI: 10.17221/119/2019-CJGPB
Apple scab caused by Venturia inaequalis has the most destructive effects among other phytopathogens in apple crops all over the world. The integration of resistance genes from local and domestic cultivars is a prerequisite for the efficient control of this disease and is a main target in efficient breeding approaches. Across Greece, many domestic apple cultivars are reported without deep knowledge about the presence and diversity of scab resistance genes. In this study, the presence of five resistance genes (Rvi2, Rvi4, Rvi6, Rvi8 and Rvi11) was evaluated across twenty local and domestic apple...
Discrimination of tobacco cultivars using SCAR and RAPD markersShort Communication
Jiuzhe Sun, Junting Wang, Dongying Su, Jinchu Yang, Erbin Wang, Shixi Wu, Meng Li, Lin Ma
Czech J. Genet. Plant Breed., 2020, 56(4):170-173 | DOI: 10.17221/120/2019-CJGPB
Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one...