Czech J. Genet. Plant Breed., 2020, 56(4):150-158 | DOI: 10.17221/113/2019-CJGPB

Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther cultureOriginal Paper

Osama Zuhair Kanbar1,2, Csaba Lantos2, Paul Karumba Chege1,2, Erzsebet Kiss3, Janos Pauk*,2
1 Doctoral School of Plant Science, Szent Istvan University, Gödöllő, Hungary
2 Departemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary
3 Genetics, Microbiology and Biotechnology Institute, Szent Istvan University, Gödöllő, Hungary

We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25-87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

Keywords: breeding; haploid; Triticum aestivum L.; wheat

Published: December 31, 2020  Show citation

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Kanbar OZ, Lantos C, Chege PK, Kiss E, Pauk J. Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture. Czech J. Genet. Plant Breed. 2020;56(4):150-158. doi: 10.17221/113/2019-CJGPB.
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    References

    1. Broughton S. (2011): The application of n-butanol improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.) genotypes. Crop and Pasture Science, 62: 813-822. Go to original source...
    2. Broughton S. (2008): Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.). Plant Cell, Tissue and Organ Culture, 95: 185-195. Go to original source...
    3. Castillo A.M., Sánchez-Díaz R.A., Vallés M.P. (2015): Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association. Frontiers in Plant Science, 6: 402. Go to original source... Go to PubMed...
    4. Chen J.F., Cui L., Malik A.A., Mbira K.G. (2011): In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell, Tissue and Organ Culture, 104: 311-319. Go to original source...
    5. De Buyser J., Henry Y., Lonnet P., Herzog R., Hespel A. (1987): Florin - a doubled haploid wheat variety developed by the anther culture method. Plant Breeding, 98: 53-56. Go to original source...
    6. Dunwell J.M. (2010): Haploids in flowering plants: origins and exploitation. Plant Biotechnology Journal, 8: 377-424. Go to original source... Go to PubMed...
    7. Dwivedi S.L., Britt A.B., Tripathi L., Sharma S., Upadhyaya H.D., Ortiz R. (2015): Haploids: constraints and opportunities in plant breeding. Biotechnology Advances, 33: 812-829. Go to original source... Go to PubMed...
    8. El-Hennawy M.A., Abdalla A.F., Shafey S.A., Al-Ashkar I.M. (2011): Production of doubled haploid wheat lines (Triticum aestivum L.) using anther culture technique. Annals of Agricultural Sciences, 56: 63-72. Go to original source...
    9. Graf R.J., Hucl P., Orshinsky B.R., Kartha K.K. (2003): "McKenzi" hard red spring wheat. Canadian Journal of Plant Science, 83: 565-569. Go to original source...
    10. Grauda D., Miķelsone A., Ļisina N., ®agata K., Ornicāns R., Fokina O., Lapiòa L., Rashal I. (2014): Anther culture effectiveness in producing doubled haploids of cereals. Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, 68: 142-147. Go to original source...
    11. Hao M., Zhang L., Luo J., Yuan Z., Yan Z., Zhang B. (2013): The genetic study utility of a hexaploid wheat DH population with non-recombinant A- and B-genomes. SpringerPlus, 2: 131. Go to original source... Go to PubMed...
    12. Holme I.B., Olesen A., Hansen N.J.P., Andersen S.B. (1999): Anther and isolated microspore culture response of wheat lines from northwestern and eastern Europe. Plant Breeding, 118: 111-117. Go to original source...
    13. Hu D.F., Yuan Z.D., Tang Y.L., Liu J.P. (1986): "Jinghua No-1" a winter-wheat variety derived from pollen sporophyte. Science in China Series B - Chemistry, Biological, Agricultural, Medical and Earth Sciences, 29: 733-745.
    14. Islam S.M.S. (2010): Effect of embryoids age, size and shape for improvement of regeneration efficiency from microspore-derived embryos in wheat (Triticum aestivum L.). Plant Omics, 3: 149-153.
    15. Islam S.M.S., Tuteja N. (2012): Enhancement of androgenesis by abiotic stress and other pretreatments in major crop species. Plant Science, 182: 134-144. Go to original source... Go to PubMed...
    16. Jauhar P.P., Xu S.S., Baenziger P.S. (2009): Haploidy in cultivated wheats: induction and utility in basic and applied research. Crop Science, 49: 737-755. Go to original source...
    17. Khiabani B.N., Vedadi C., Rahmani E., Shalmani M.A.M. (2008): Response of some Iranian wheat genotypes to anther culture system. Indian Journal of Biotechnology, 7: 531-535.
    18. Kim K.M., Baenziger P.S. (2005): A simple wheat haploid and doubled haploid production system using anther culture. In Vitro Cellular and Developmental BiologyPlant, 41: 22-27. Go to original source...
    19. Kondic-Spika A.Ð., Kobiljski B.Ð., Hristov N.S. (2008): Efficiency of anther culture technique in the production of wheat double haploids. Zbornik Matice Srpske za Prirodne Nauke, 2008: 35-40. Go to original source...
    20. Kondic-Spika A., Vukosavljev M., Kobiljski B., Hristov N. (2011): Relationships among androgenetic components in wheat and their responses to the environment. Journal of Biological Research, 16: 217-223.
    21. Kush G.S., Virmani S.S. (1996): Haploids in plant breeding. In: Mohan Jain S., Sopory S.K., Veilleux R.E. (eds.): In vitro Production in Higher Plants. Dordrecht/Boston/ London, Kluwer Academic Publishers: 11-34.
    22. Lantos C., Pauk J. (2016): Anther culture as an effective tool in winter wheat (Triticum aestivum L.) breeding. Russian Journal of Genetics, 52: 794-801. Go to original source...
    23. Lantos C., Weyen J., Orsini J.M., Gnad H., Schlieter B., Lein V., Kontoeski S., Jacobi A., Mihaly R., Broughton S., Pauk J. (2013): Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes. Plant Breeding, 132: 149-154. Go to original source...
    24. Mahato A., Chaudhary H.K. (2019): Auxin induced haploid induction in wide crosses of durum wheat. Cereal Research Communications, 47: 552-565. Go to original source...
    25. Masojc P.O., Lukow M., McKenzie R.I.H., Howes N.K. (1993): Responsiveness to anther culture in cultivars and F 1 crosses of spring wheat. Canadian Journal of Plant Science, 73: 777-783. Go to original source...
    26. Navarro-Alvarez W., Baenziger P.S., Eskridge K.M., Hugo M., Gustafson V.D. (1994): Addition of colchicine to wheat anther culture media to increase doubled haploid plant production. Plant Breeding, 112: 192-198. Go to original source...
    27. Niu Z., Jiang A., Abu Hammad W., Oladzadabbasabadi A., Xu S.S., Mergoum M., Elias E.M. (2014): Review of doubled haploid production in durum and common wheat through wheat × maize hybridization. Plant Breeding, 133: 313-320. Go to original source...
    28. Pauk J., Kertész Z., Beke B., Bóna L., Csősz M., Matuz J. (1995): New winter wheat variety - "GK Délibáb" developed via combining conventional breeding and in vitro androgenesis. Cereal Research Communications, 23: 251-256.
    29. Pauk J., Mihály R., Puolimatka M. (2003): Protocol of wheat (Triticum aestivum L.) anther culture. In: Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (eds.): Doubled Haploid Production in Crop Plants, a Manual. Dordrecht, Springer: 59-64. Go to original source...
    30. R Core Team (2019): R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria.
    31. Sadasivaiah R.S., Perkovic S.M., Pearson C., Postman B., Beres B.L. (2004): Registration of "AC Andrew" wheat. Crop Science, 44: 696-697. Go to original source...
    32. Sharma S., Sethi G.S., Chaudhary H.K. (2005): Influence of winter and spring wheat genetic backgrounds on haploid induction parameters and trait correlations in the wheat × maize system. Euphytica, 144: 199-205. Go to original source...
    33. Shi Y.G., Lian Y., Shi H.W., Wang S.G., Fan H., Sun D.Z., Jing R.L. (2019): Dynamic analysis of QTLs for green leaf area duration and green leaf number of main stem in wheat. Cereal Research Communications, 47: 250-263. Go to original source...
    34. Sorrells M.E., Gustafson J.P., Somers D., Chao S., Benscher D., Guedira-Brown G., Huttner E., Kilian A., McGuire P.E., Ross K., Tanaka J., Wenzl P., Williams K., Qualset C.O. (2011): Reconstruction of the synthetic W7984 × Opata M85 wheat reference population. Genome, 54: 1-8. Go to original source... Go to PubMed...
    35. Suenaga K., Morshedi A.R., Darvey N.L. (1997): Haploid production of Australian wheat (Triticum aestivum L.) cultivars through wheat × maize (Zea mays L.) crosses. Australian Journal of Agricultural Research, 48: 1207- 1212. Go to original source...
    36. Thomas W.T.B., Forster B.P., Gertsson B. (2003): Doubled haploids in breeding. In: Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (eds.): Doubled Haploid Production in Crop Plants: A Manual. Norwell, Kluwer Academic Publishers: 337-350. Go to original source...
    37. Trottier M.C., Collin J., Comeau A. (1993): Comparison of media for their aptitude in wheat anther culture. Plant Cell, Tissue and Organ Culture, 35: 59-67. Go to original source...
    38. Tuvesson S., Ljungberg A., Johansson N., Karlsson K.E., Suijs L.W., Josset J.P. (2000): Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method. Plant Breeding, 119: 455-459. Go to original source...
    39. Tuvesson S.A., Von Post R., Ljungberg A. (2003): Wheat anther culture. In: Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. (eds.): Doubled Haploid Production in Crop Plants, a Manual. Dordrecht/Boston/London, Kluwer Academic Publishers: 71-76. Go to original source...
    40. Wędzony M., Forster B.P., Żur I., Golemiec E., SzechyńskaHebda M., Dubas E., Gotębiowska G. (2009): Progress in doubled haploid technology in higher plants. In: Touraev A., Forster B.P., Jain S.M. (eds.): Advances in Haploid Production in Higher Plants. Dordrecht, Springer: 1-33. Go to original source...
    41. Yan G., Liu H., Wang H., Lu Z., Wang Y., Mullan D., Hamblin J., Liu C. (2017): Accelerated generation of selfed pure line plants for gene identification and crop breeding. Frontiers in Plant Science, 8: 1786. Go to original source... Go to PubMed...
    42. Ziegler G., Dressler K., Hess D. (1990): Investigations on the anther culturability of four German spring wheat cultivars and the influence of light on regeneration of green vs. albino plants. Plant Breeding, 105: 40-46. Go to original source...

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