Czech Journal of Genetics and Plant Breeding, 2020 (vol. 56), issue 2
A 1BL/1RS translocation contributing to kernel length increase in three wheat recombinant inbred line populationsOriginal Paper
Shui Qin Li, Hua Ping Tang, Han Zhang, Yang Mu, Xiu Jin Lan, Jian Ma
Czech J. Genet. Plant Breed., 2020, 56(2):43-51 | DOI: 10.17221/79/2019-CJGPB 
The 1BL/1RS wheat-rye translocation has been widely utilized in wheat genetic improvement and breeding programs. Our understanding on the effects of the 1BL/1RS translocation on wheat kernel size (e.g. length and width) is limited despite of numerous studies reporting about the effects on kernel weight. Here, we identified a wheat 1BL/1RS translocation line 88-1643 with higher kernel length (KL) using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers. To detect the possible role of the 1BL/1RS translocation in KL, kernel width (KW), and thousand-kernel weight (TKW), three recombinant...
The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.)Original Paper
Cong Cong Yang, Jian Ma, Cong Li, Min Sun, Ya Ya Zou, Ting Li, Yang Mu, Hua Ping Tang, Xiu Jin Lan
Czech J. Genet. Plant Breed., 2020, 56(2):52-61 | DOI: 10.17221/35/2019-CJGPB
Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into...
Genetic diversity of released Malaysian rice varieties based on single nucleotide polymorphism markersOriginal Paper
Shahril Ab Razak, Nor Helwa Ezzah Nor Azman, Rahiniza Kamaruzaman, Shamsul Amri Saidon, Muhammad Fairuz Mohd Yusof, Siti Norhayati Ismail, Mohd Azwan Jaafar, Norzihan Abdullah
Czech J. Genet. Plant Breed., 2020, 56(2):62-70 | DOI: 10.17221/58/2019-CJGPB
Understanding genetic diversity is a main key for crop improvement and genetic resource management. In this study, we aim to evaluate the genetic diversity of the released Malaysian rice varieties using single nucleotide polymorphism (SNP) markers. A total of 46 released Malaysian rice varieties were genotyped using 1536 SNP markers to evaluate their diversity. Out of 1536 SNPs, only 932 SNPs (60.7%) represented high quality alleles, whereas the remainder either failed to amplify or had low call rates across the samples. Analysis of the 932 SNPs revealed that a total of 16 SNPs were monomorphic. The analysis of the SNPs per chromosome revealed that...
Identification of rare traditional grapevine cultivars using SSR markers and their geographical location within the Czech RepublicOriginal Paper
Kateřina Baránková, Radek Sotolář, Miroslav Baránek
Czech J. Genet. Plant Breed., 2020, 56(2):71-78 | DOI: 10.17221/61/2019-CJGPB
The designation of traditional varieties of grapevine is usually based on verbal information or very dated records. Old rare cultivars found in the Czech Republic were identified by Simple Sequence Repeat (SSR) analysis, a generally accepted method for cultivar identification. These cultivars are primarily maintained in a national collection of genetic resources. Finally a total of 102 candidate genotypes was selected where 49 traditional varieties were identified on the base of nine SSR loci compared with the European Vitis Database. Thirty-six items were registered under the correct designation. The remaining genotypes included four clones, and two...
Genetic characterisation of the petal spot phenotype for Gossypium arboreum accession PI 408798Original Paper
John E. Erpelding
Czech J. Genet. Plant Breed., 2020, 56(2):79-83 | DOI: 10.17221/88/2019-CJGPB
The occurrence of floral petal spots is common among flowering plants and plays a major role in attracting pollinators. Cotton genotypes having large red petal spots are frequent in the United States Department of Agriculture, National Plant Germplasm System Gossypium arboreum (L.) collection. One accession, PI 408798, showed a unique faint red petal spot phenotype. To genetically characterise this trait, a F2 population of 226 plants was developed by crossing PI 408798 with G. arboreum accession PI 529714 that lacked pigmented petal spots. From the population, 161 plants showed the presence of faint red...
Ing. Pavel Bartoš, DrSc. - 90th birthdayBiographical Notice
Alena Hanzalová, Hana Reimarová
Czech J. Genet. Plant Breed., 2020, 56(2):84 | DOI: 10.17221/21/2020-CJGPB
List of field crop varieties registered in the Czech Republic in 2019New Varieties
Tomáš Mezlík
Czech J. Genet. Plant Breed., 2020, 56(2):85-86 | DOI: 10.17221/19/2020-CJGPB