Czech Journal of Genetics and Plant Breeding, 2019 (vol. 55), issue 4
Index of Volume 55Index
editors
Czech J. Genet. Plant Breed., 2019, 55(4):I-IV | DOI: 10.17221/12603-CJGPB 
Evaluation of diversity and resistance of maize varieties to Fusarium spp. causing ear rot in maize under conditions of natural infectionOriginal Paper
Yong Gang Li, Dan Jiang, Lan Kun Xu, Si Qi Zhang, Ping Sheng Ji, Hong Yu Pan, Bai Wen Jiang, Zhong Bao Shen
Czech J. Genet. Plant Breed., 2019, 55(4):131-137 | DOI: 10.17221/81/2018-CJGPB
Fusarium ear rot in maize (Zea mays L.) is a serious disease in all maize-growing areas worldwide. A total of 454 fungal strains were isolated from 69 commercial maize hybrids grown in Harbin, China, and comprised Fusarium subglutinans (34.8%), F. proliferatum (31.3%), F. verticillioides (20%), F. graminearum (9.7%), and F. equiseti (4.2%). Among them, a complex of multiple species, F. subglutinans, F. proliferatum, and F. verticillioides are the dominant fungi causing ear rot. Among 59 commercial maize hybrids, eleven hybrids (18.6%) were found to be highly resistant...
Marker-trait associations of yield related traits in bread wheat (Triticum aestivum L.) under a semi-arid climateOriginal Paper
Salem Marzougui, Mohamed Kharrat, Mongi ben Younes
Czech J. Genet. Plant Breed., 2019, 55(4):138-145 | DOI: 10.17221/154/2018-CJGPB
Identifying QTLs (quantitative trait loci) that control yield related traits under a stressed environment is very useful for marker-assisted selection (MAS). Marker-trait associations (MTA) for several agro-morphological traits were performed with 130 Tunisian and exotic spring bread wheat (Triticum aestivum L.) accessions under a semi-arid climate in El Kef, Tunisia. Grain yield and other important traits were evaluated. A population structural analysis identified two sub populations. In total, 29 MTAs were detected at -log P ≥ 3 using an MLM (mixed linear model), and only 5 MTAs with -log P ≥ 4. The locus on...
QTL mapping of physiological traits at the booting stage in rice under low temperature combined with nitrogen fertilizationOriginal Paper
Shu Ming Yang, Fei Fei Zhang, Su Hua Zhang, Gui Yong Li, Li Qiong Zeng, Guan Suo Liu, Xiao Fen Yu, Xue Li Qiu
Czech J. Genet. Plant Breed., 2019, 55(4):146-155 | DOI: 10.17221/67/2018-CJGPB
Further dissection of physiological molecular mechanisms is indispensable to alleviate rice yield losses resulting from cold injury. By using 105 near-isogenic lines (NILs) derived from a backcross between cv. Lijiangxintuanheigu (LTH) and cv. Towada, we detected quantitative trait loci (QTLs) for physiological traits of the rice flag leaf, based on polymorphic simple sequence repeat (SSR) markers, inclusive composite interval mapping (ICIM), mixed composite interval mapping (MCIM) approaches and phenotypic value subjected to combine with cold-water stress and three nitrogen application rates. By using ICIM, a total of 34 QTLs with additive effects...
Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiencyOriginal Paper
Jia-Hao Su, Tian-Hui Bai, Fei Wang, Ai-Ke Bao
Czech J. Genet. Plant Breed., 2019, 55(4):156-161 | DOI: 10.17221/134/2018-CJGPB
Alfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to short-term salinity or drought. In this study, the growth performances of two transgenic lines were further investigated under long-term salinity or drought conditions, as well as under phosphate deficiency (low-Pi). Compared with wild-type (WT) plants, the transgenic alfalfa showed better...
Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryosShort Communication
Miroslav Klíma, Eva Jozová, Irena Jelínková, Vratislav Kučera, Shengwu Hu, Vladislav Čurn
Czech J. Genet. Plant Breed., 2019, 55(4):162-165 | DOI: 10.17221/94/2018-CJGPB
Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype...
An RNA-Seq analysis of the peach transcriptome with a focus on genes associated with skin colourShort Communication
Ping He, Linguang Li, Haibo Wang, Yuansheng Chang
Czech J. Genet. Plant Breed., 2019, 55(4):166-169 | DOI: 10.17221/90/2018-CJGPB
Red pigmentation of the skin is an important consumer trait in peach (Prunus persica). The pigment consists largely of anthocyanin. Here, a transcriptomic contrast, based on RNA-Seq technology, was drawn between a white-skinned (Feitao) and a red-skinned (Qiuxue) peach cultivar. The analysis identified 2407 genes as differentially transcribed in the fruit skin of the two cultivars. Among these were a number of genes known to contribute to anthocyanin synthesis. A quantitative real-time PCR assay was used to validate the RNA-Seq-based estimates of transcript abundance for 14 differentially transcribed genes. Anthocyanin synthesis was observed...