Czech Journal of Genetics and Plant Breeding, 2015 (vol. 51), issue 4
INDEX OF VOLUME 51, AUTHORS INDEX
editors
Czech J. Genet. Plant Breed., 2015, 51(4):I-IV | DOI: 10.17221/8579-CJGPB 
Transgenic flax/linseed (Linum usitatissimum L.) - expectations and realityReview
Michaela LUDVÍKOVÁ, Miroslav GRIGA
Czech J. Genet. Plant Breed., 2015, 51(4):123-141 | DOI: 10.17221/104/2015-CJGPB
This review summarizes the history, important milestones, current status and perspectives of biotech flax/linseed (Linum usitatissimum L.), supplemented with some of our original research, breeding and data on environmentalsafety. We show how recent biotechnology methods and genetic engineering contributed to the flax/linseed breeding in order to speed up the breeding process (doubled haploids technology; in vitro selection with the use of pathogenic toxins or heavy metals; genetic transformation) and for the creation of new flax/linseed cultivars. The focus is laid on genetic engineering which represents an excellent technology to...
Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindricaOriginal Paper
Harinder Kumar CHAUDHARY, Anima MAHATO, Vineeta KAILA, Shoukat Ahmad RATHER, Tisu TAYENG
Czech J. Genet. Plant Breed., 2015, 51(4):142-147 | DOI: 10.17221/218/2014-CJGPB
Doubled haploidy breeding protocols have revolutionized the varietal development programmes in bread wheat, however, the protocols have not proved much efficient in durum wheat. Presently, the durum wheat × maize system is being widely followed for haploid induction but the frequency of haploid development is very poor which has limited its application in durum wheat improvement programmes. In order to formulate an efficient wide hybridization-mediated approach for haploid induction in durum wheat, different genotypes of durum wheat were subjected to hybridization with Imperata cylindrica, a wild perennial grass for the first time in this...
Stem rust resistance in 1BL.1RS and 2RL.2BS double wheat-rye translocation linesOriginal Paper
Mahbubjon RAHMATOV, Larisa GARKAVA-GUSTAVSSON, Ruth WANYERA, Brian STEFFENSON, Matthew ROUSE, Eva JOHANSSON
Czech J. Genet. Plant Breed., 2015, 51(4):148-154 | DOI: 10.17221/80/2015-CJGPB
The wheat stem rust pathogen, Puccinia graminis f.sp. tritici, is a significant and devastating disease of wheat crops worldwide. Wheat has many wild relatives in which to source new resistance genes, including the cereal crop of rye in the tertiary genepool. The aim of this study was to assess the reaction of 1BL.1RS and 2RL.2BS double wheat-rye translocation lines to virulent stem rust races from Africa and North America. BC1F3 and BC1F4 populations from a cross between the line KR99-139 (a double wheat-rye translocation line with 1BL.1RS and 2RL.2BS) and the bread wheat cultivar Topper...
Selecting plants with increased total polyphenol oxidases in the genus TrifoliumOriginal Paper
Hana JAKEŠOVÁ, Jana ŘEPKOVÁ, Jan NEDĚLNÍK, David HAMPEL, Jana DLUHOŠOVÁ, Martina SOLDÁNOVÁ, Martina OŠŤÁDALOVÁ
Czech J. Genet. Plant Breed., 2015, 51(4):155-161 | DOI: 10.17221/107/2015-CJGPB
One of the aims in red clover (Trifolium pratense) breeding is to increase the polyphenol oxidase (PPO) activity, which may effectively reduce protein breakdown in silage and when cattle are fed by fresh clover. We analysed total PPO activity spectrophotometrically and on the level of gene expression using real-time quantitative PCR in single plants derived from an interspecific T. pratense × T. medium hybrid. Experiments were performed for two years and evaluated according to the general linear model with three factors (family, year, and cut). The analysis revealed considerable variability in total PPO activity between individuals...
New resistance sources to Russian wheat aphid (Diuraphis noxia) in Swedish wheat substitution and translocation lines with rye (Secale cereale) and Leymus mollisShort Communication
Staffan C. ANDERSSON, Eva JOHANSSON, Michael BAUM, Fawzi RIHAWI, Mustapha EL BOUHSSINI
Czech J. Genet. Plant Breed., 2015, 51(4):162-165 | DOI: 10.17221/72/2015-CJGPB
Russian wheat aphid is an important pest in many wheat growing countries of the world. Different resistance genes to the pest have previously been identified. However, some biotypes of the pest are virulent to most of these genes. Swedish wheat-rye and wheat-Leymus, consisting of 235 accessions with substitutions and translocations, were investigated for resistance to the aphid. A total of 86 accessions were shown to be either resistant or moderately resistant. Resistance was found particularly in accessions having substitutions of 1R instead of 1D [1R(1D) or 1R(1D) + 6R(6D)], in translocations to 3D or 5A (3DL.3RS and 5AL.5RS) and in accessions...
Eyespot resistance gene Pch1 and methods of study of its effectiveness in wheat cultivarsScientific Reports
Veronika Dumalasová, Jana Palicová, Alena Hanzalová, Irena Bížová, Leona Leišová-Svobodová
Czech J. Genet. Plant Breed., 2015, 51(4):166-173 | DOI: 10.17221/157/2015-CJGPB
The scientific report presents results of our studies on the gene Pch1, conferring resistance to eyespot disease in wheat, caused by the fungus Occulimacula spp. The presence of the gene Pch1 in wheat cultivars was analysed using the molecular marker Xorw1. In total 106 wheat cultivars registered in the Czech Republic and 54 breeding lines were tested. The gene Pch1 was found in the cultivars Annie, Beduin, Hermann, Iridium, Manager and Princeps and in three breeding lines. Some of these cultivars were tested for eyespot resistance in field trials and showed a high level of resistance. Cv. Hermann was the...