Czech J. Genet. Plant Breed., 2015, 51(4):166-173 | DOI: 10.17221/157/2015-CJGPB

Eyespot resistance gene Pch1 and methods of study of its effectiveness in wheat cultivarsScientific Reports

Veronika Dumalasová1, Jana Palicová1, Alena Hanzalová1, Irena Bížová2, Leona Leišová-Svobodová1
1 Crop Research Institute, Prague-Ruzyně, Czech Republic
2 Research Centre SELTON, Station Úhřetice, Czech Republic

The scientific report presents results of our studies on the gene Pch1, conferring resistance to eyespot disease in wheat, caused by the fungus Occulimacula spp. The presence of the gene Pch1 in wheat cultivars was analysed using the molecular marker Xorw1. In total 106 wheat cultivars registered in the Czech Republic and 54 breeding lines were tested. The gene Pch1 was found in the cultivars Annie, Beduin, Hermann, Iridium, Manager and Princeps and in three breeding lines. Some of these cultivars were tested for eyespot resistance in field trials and showed a high level of resistance. Cv. Hermann was the most resistant winter wheat cultivar in our experiments in the last two years. The method of artificial infection by Oculimacula spp. was optimized.

Keywords: eyespot; molecular markers; Pch1 gene; resistance; wheat

Published: December 31, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dumalasová V, Palicová J, Hanzalová A, Bížová I, Leišová-Svobodová L. Eyespot resistance gene Pch1 and methods of study of its effectiveness in wheat cultivars. Czech J. Genet. Plant Breed. 2015;51(4):166-173. doi: 10.17221/157/2015-CJGPB.
Download citation

References

  1. Bundessortenamt (2015): Beschriebende Sortenliste. Getreide, Mais, Öl- und Faserpflanzen, Leguminosen, Rüben, Zwischenfrüchte. Hannover. Available at https://www.bundessortenamt.de/internet30/fileadmin/Files/PDF/bsl_getreide_2015.pdf
  2. Burt C., Hollins T.W., Powell N., Nicholson P. (2010): Differential seedling resistance to the eyespot pathogens, Oculimacula yallundae and Oculimacula acuformis, conferred by Pch2 in wheat and among accessions of Triticum monococcum. Plant Pathology, 59: 819-828. Go to original source...
  3. Burt C., Hollins T.W., Nicholson P. (2011): Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez. Theoretical and Applied Genetics, 122: 119-128. Go to original source... Go to PubMed...
  4. Crous P.W., Groenewald J.Z.E., Gams W. (2003): Eyespot of cereals revisited: ITS phylogeny reveals new species relationships. European Journal of Plant Pathology, 109: 841-850. Go to original source...
  5. Dumalasová V., Leišová-Svobodová L., Sumíková T., Bartoš P. (2013): Variety resistance of wheat to eyespot. Úroda, 6: 24-27.
  6. Leonard J.M., Watson CH.J.W., Carter A.H., Hansen J.L., Zemetra R.S., Santra D.K., Cambbell K.G., Riera-Lizarazu O. (2008): Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1. Theoretical and Applied Genetics, 116: 261-270. Go to original source... Go to PubMed...
  7. Maia N. (1967): Obtention de blés tendres résistants au piétin-verse (Cercosporella herpotrichoides) par croisements interspécifiques blés x Aegilops. Comptes Rendus de l'Academie d'Agriculture de France, 53: 149-154.
  8. Matusinsky P., Mikolasova R., Klem K., Spitzer T. (2009): Eyespot infection risks on wheat with respect to climatic conditions and soil management. Journal of Plant Pathology, 91: 93-101.
  9. McMillin D.E., Allan R.E., Roberts D.E. (1986): Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theoretical and Applied Genetics, 72: 743-747. Go to original source... Go to PubMed...
  10. Meyer N., Lind V., Heindorf M., Korzun V., Triedr W., Ordon F. (2011): Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat. Euphytica, 177: 267-275. Go to original source...
  11. Wei L., Muranty H., Zhang H. (2011): Advances and prospects in wheat eyespot research: Contribution from genetics and molecular tools. Journal of Phytopathology, 159: 457-470. Go to original source...
  12. Yildirim A., Jones S.S., Murray T.D. (1998): Resistance of Dasypyrum villosum to the cereal eyespot pathogens, Tapesia yallundae and Tapesia acuformis. Euphytica, 103: 203-209. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.