Czech Journal of Genetics and Plant Breeding, 2015 (vol. 51), issue 3

QTL mapping for adult-plant leaf rust resistance genes in Chinese wheat cultivar Weimai 8Original Paper

Jiazhen WANG, Zaifeng LI, Lingzhi SHI, Lin ZHU, Zhikuan REN, Xing LI, Daqun LIU, Syed Jawad Ahmad SHAH

Czech J. Genet. Plant Breed., 2015, 51(3):79-85 | DOI: 10.17221/51/2015-CJGPB  

The wheat cultivar Weimai 8 is a good source of adult-plant resistance to leaf rust in China. In order to understand the genetic background of the resistance genes, 179 F2:3 families derived from the cross Weimai 8 and Zhengzhou 5389 (susceptible to leaf rust) were used to construct a linkage map. Results showed that there was a major QTL on chromosome 2AS, temporarily named QLr.hbau-2AS, responsible for the resistance. QLr.hbau-2AS from the resistant parent Weimai 8 was between the SSR markers Xcfd36 and Xbarc1138, with an interval length of 2.58 cM. In the 2010-2011, 2011-2012 and 2012-2013 crop seasons, it explained...

Response of selected winter wheat cultivars to inoculation with different Mycosphaerella graminicola isolatesOriginal Paper

Václav ŠÍP, Jana CHRPOVÁ, Jana PALICOVÁ

Czech J. Genet. Plant Breed., 2015, 51(3):86-95 | DOI: 10.17221/44/2015-CJGPB  

Five winter wheat cultivars, differing in resistance to Septoria tritici blotch (STB), were spray inoculated under field conditions for two years and at two locations with nine Mycosphaerella graminicola isolates and a mixture of isolates that were obtained from different regions of the Czech Republic. Main aims of this study were (i) to compare isolate, host cultivar and environmental effects on five traits indicative of STB severity and (ii) to analyse pathogen aggressiveness and host-pathogen relations for improving evaluation of cultivar resistance. ANOVA showed in all traits, except the reduction in 1000 grain weight,...

Agro-morphological and molecular diversity in castor (Ricinus communis L.) germplasm collected from Andaman and Nicobar Islands, IndiaOriginal Paper

Meena KANTI, Kammili ANJANI, Betha USHA KIRAN, K. VIVEKANANDA

Czech J. Genet. Plant Breed., 2015, 51(3):96-109 | DOI: 10.17221/205/2014-CJGPB  

Castor (Ricinus communis L.) is an industrial oilseed crop grown worldwide. Its oil with more than 80% ricinoleic acid makes it a chief raw material for numerous industrial applications and biofuel production. Castor grows in a wild form across India including Andaman & Nicobar Islands, which are geographically quite isolated from mainland India. Thirty-three accessions growing in isolation in these Islands were used in the present study. Genetic diversity among these accessions was assessed using 18 agro-morphological traits and 29 EST-SSR markers. High agro-morphological and molecular variability was observed among these accessions....

Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probesOriginal Paper

Chao-Wen SHE, Xiang-Hui JIANG

Czech J. Genet. Plant Breed., 2015, 51(3):110-116 | DOI: 10.17221/32/2015-CJGPB  

The mitotic chromosomes of Lablab purpureus (L.) Sweet were characterised using sequential combined propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) (CPD) staining and fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. The detailed karyotype of this species was established using prometaphase chromosomes. After CPD staining, CPD and DAPI⁺ bands were shown simultaneously. CPD bands occurred in the proximal regions of the long arms of all chromosome pairs and at all 45S rDNA sites, while the DAPI⁺ bands appeared in all centromeres. FISH with rDNA probes revealed one 5S locus and...

Morphological characterization and turf performance of Paula hard fescue and Casero colonial bentgrass selections under low maintenance conditionsOriginal Paper

Jose Alberto OLIVEIRA PRENDES, Pedro PALENCIA

Czech J. Genet. Plant Breed., 2015, 51(3):117-122 | DOI: 10.17221/78/2015-CJGPB  

Planting grasses that require low maintenance is a good option for reducing the management input required. The objective of this study was to obtain information about turfgrass characteristics based on spaced-plant characterization and turf performance in turf plots of two selections (Paula hard fescue and Casero colonial bentgrass) recently released for public distribution by the Plant Production Area of the University of Oviedo. The plants were grown under conditions of no irrigation, no fertilizer, no pesticide application and minimal mowing over a 3-year period (2012-2014). The following morphological measurements were done in a spaced-plant nursery:...