Czech Journal of Genetics and Plant Breeding, 2013 (vol. 49), issue 3
Newly discovered genes for resistance to powdery mildew in the subtelomeric region of the short arm of barley chromosome 7HOriginal Paper
Martina SOLDÁNOVÁ, Jan IŠTVÁNEK, Jana ŘEPKOVÁ, Antonín DREISEITL
Czech J. Genet. Plant Breed., 2013, 49(3):95-102 | DOI: 10.17221/33/2013-CJGPB 
Two dominant genes for resistance to powdery mildew (caused by Blumeria graminis f.sp. hordei) from the PI296825 and PI466461 accessions of wild barley (Hordeum vulgare subsp. spontaneum) were identified close to the subtelomeric region of the short arm of chromosome 7H. Genetic analyses predicted two resistance loci in F2 populations established from crosses between each of the two accessions and the winter barley (H. vulgare) variety Tiffany. Genetic mapping revealed a highly effective (52% of phenotypic variation) resistance gene from PI296825 located between the markers GBMS192...
Physiological specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2009-2011Original Paper
Alena HANZALOVÁ, Pavel BARTOŠ, Taťána SUMÍKOVÁ
Czech J. Genet. Plant Breed., 2013, 49(3):103-108 | DOI: 10.17221/221/2012-CJGPB
In 2009-2011 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained in different parts of the Czech Republic. A total of 164 wheat leaf rust isolates were analysed. Resistance gene Lr9 was effective to 98% of all tested isolates. No virulence to Lr19 was found. Gene Lr24 was effective to 93% of isolates. A lower frequency...
Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheatOriginal Paper
Aleksandra PIETRUSIŃSKA, Paweł Częstobor CZEMBOR, Jerzy Henryk CZEMBOR
Czech J. Genet. Plant Breed., 2013, 49(3):109-115 | DOI: 10.17221/150/2012-CJGPB
Two effective resistance genes were introduced, one for leaf rust (Lr39) and the other for powdery mildew (Pm21), into the susceptible German wheat cv. Lexus. Molecular selection of plant material was carried out using closely linked markers to the introduced genes (foreground selection). In addition, for the BC1F1 population, background selection was carried out using AFLP markers that were distributed randomly throughout the wheat genome. Moreover, resistance tests were conducted using natural pathogen populations of Puccinia triticina and Blumeria graminis. The use of molecular markers for...
Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markersOriginal Paper
Milena MUSILOVÁ, Václav TROJAN, Tomáš VYHNÁNEK, Ladislav HAVEL
Czech J. Genet. Plant Breed., 2013, 49(3):116-122 | DOI: 10.17221/160/2012-CJGPB
Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which cause purple, blue or yellow coloration. The first step before the introduction of these traits into individual wheat cultivars is the characterization of relationships and the possibility of new gene combinations. In this study, relationships among 24 genotypes with different types of caryopsis colour were investigated by means of microsatellite markers....
An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explantsOriginal Paper
Na Tian, Shuoqian Liu, Hiengming Ting, Jianan Huang, Sander Van Der Krol, Harro Bouwmeester, Zhonghua Liu
Czech J. Genet. Plant Breed., 2013, 49(3):123-129 | DOI: 10.17221/105/2012-CJGPB
Transformation of Artemisia annua, which produces the sesquiterpenoid endoperoxide artemisinin widely used for the treatment of malaria, has been hampered by the low efficiency of adventitious shoot and root formation on a selective medium containing additional compounds for Agrobacterium decontamination. Here we identified several factors which were all shown to be of importance for optimization of Artemisia annua transformation. Results indicated that stem internodes showed better resistance capacity to Agrobacterium decontaminator than leaves did. Agrobacterium tumefaciens with an optical density (OD)...