Czech Journal of Genetics and Plant Breeding, 2013 (vol. 49), issue 2
Integration and characterization of T-DNA insertion in upland cottonOriginal Paper
Xiaojie YANG, Fuguang LI, Xueyan ZHANG, Kun LIU, Qianhua WANG, Chaojun ZHANG, Chuanliang LIU, Wei ZHU, Guofang SHAN, Chee-Kok CHIN, Weiping FANG
Czech J. Genet. Plant Breed., 2013, 49(2):51-57 | DOI: 10.17221/217/2012-CJGPB 
Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the...
Protein extract of tobacco expressing StoVe1 gene inhibits Verticillium dahliae proliferationOriginal Paper
Shui-Ping LIU, Yong-Bo HONG, Zhen WU, Yong-Shuo MA, Deng-Wei JUE, Chao XIE, Yan-Ping ZHU, Min CHEN, Qing YANG
Czech J. Genet. Plant Breed., 2013, 49(2):58-64 | DOI: 10.17221/165/2012-CJGPB
Verticillium dahliae is a principal pathogen causing verticillium wilt in Solanaceae crops. StoVe1 is a gene resisting to verticillium wilt isolated from Solanum torvum. In order to generate resistant tobacco plants, StoVe1 was inserted in the orientation behind CaMV 35S promoter of vector pGS and this construct was introduced into tobacco by Agrobacterium-mediated transformation. A total of 12 kanamycin-resistant plants were generated and 7 independent transgenic lines were identified by PCR analysis. Quantitative RT-PCR analysis showed that the levels of StoVe1 transcript in transgenic...
Evaluation of the Prunus interspecific progenies for resistance to Plum pox virusOriginal Paper
Jaroslav SALAVA, Jaroslav POLÁK, Ivan OUKROPEC
Czech J. Genet. Plant Breed., 2013, 49(2):65-69 | DOI: 10.17221/252/2012-CJGPB
Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worldwide the most devastating for stone fruit production. Until now, good sources of resistance to PPV within the peach group have not been available. There are no commercial cultivars of peach that are resistant to PPV. Other Prunus species are known to show varying levels of resistance. Interspecific hybrids GF 677 (Prunus amygdalus × P. persica) and Cadaman (P. davidiana × P. persica) were revealed to be resistant to PPV. The resistance to a Dideron isolate of the descendants of Cresthaven × GF 677 and...
Competition ability of selected amenity varieties of Festuca rubra in mixture with Deschampsia caespitosaOriginal Paper
Miluše SVOBODOVÁ, Jaroslav MARTINEK, Tereza KRÁLÍČKOVÁ, Ivo NAŠINEC, Jaromír ŠANTRŮČEK
Czech J. Genet. Plant Breed., 2013, 49(2):70-76 | DOI: 10.17221/86/2011-CJGPB
The aim of the research was to evaluate the competition capacity and find suitable proportion of different types of Festuca rubra and Deschampsia caespitosa in grass mixtures for decorative lawns. A field experiment with Deschampsia caespitosa cv. Kometa in monoculture or in mixtures with Festuca rubra L. varieties Viktorka (ssp. trichophylla), Barborka (ssp. commutata) and Petruna (ssp. rubra) was established in Větrov in 2007. The sowing rate was 40 000 viable seeds per m2 (0, 25, 50 or 75% of F. rubra). The sward was mown at 3 cm. The number and weight of tillers...
Seedling resistance to Stagonospora nodorum blotch in wheat genotypesShort Communication
Mónika CSÉPLŐ, Mária CSŐSZ, Mariann GÁL, Ottó VEISZ, Gyula VIDA
Czech J. Genet. Plant Breed., 2013, 49(2):77-85 | DOI: 10.17221/69/2011-CJGPB
In two independent experiments set up in the greenhouse the seedling resistance to Stagonospora nodorum blotch was investigated in 92 varieties, breeding lines and genotypes with a known genetic background. The greatest area under the disease progress curve calculated from lesion type was 37.06, while in the case of the most resistant genotype this value was 0.38. Many of the lines and varieties bred in Martonvásár proved to have excellent resistance in terms of both percentage of infected leaf area and lesion type. Observations indicate that, depending on the aim of the experiment, the efficient selection of breeding lines is possible in the seedling...
EMS induced intercellular chromatin transmigration in Papaver somniferum L.Short Communication
Girjesh KUMAR, Sana NASEEM
Czech J. Genet. Plant Breed., 2013, 49(2):86-89 | DOI: 10.17221/85/2012-CJGPB
The phenomenon of chromatin migration was observed during microsporogenesis in an ethyl methane sulphonate (EMS) treated population of poppy, which is an important medicinal plant. Cytomixis occurred through a cytoplasmic channel or by direct fusion of pollen mother cells (PMCs); the former was more recurring than the latter. The process was associated with irregular meiosis. PMCs with differing chromosome numbers from the normal diploid number (2n = 22) through cytomixis may lead to the production of aneuploid and polyploid gametes. An increase in the concentration of EMS had a positive effect on the percentage of PMCs showing cytomixis....
Development of the new winter wheat variety Skorpion with blue grainScientific Reports
Petr MARTINEK, Miroslav ŠKORPÍK, Jana CHRPOVÁ, Pavel FUČÍK, Josef SCHWEIGER
Czech J. Genet. Plant Breed., 2013, 49(2):90-94 | DOI: 10.17221/7/2013-CJGPB
Breeding wheat with blue grain was conducted at the Crop Research Institute in Prague. Initial donor material came from the legacy of Erich von Tschermak-Seysenegg. Long-term crosses with a series of winter wheat varieties were made with the aim of transferring blue grain colour into cultivated varieties. The prospective material was later handed over to Agrotest Fyto, Ltd., Kroměříž, where line no. 6 was selected from the population RU 440. At the end of 2011, the new winter wheat variety Skorpion with blue grain was registered in Austria. It is intended for special use in the food industry. The anthocyanins which it contains are considered to offer...