Czech J. Genet. Plant Breed., 2013, 49(2):86-89 | DOI: 10.17221/85/2012-CJGPB

EMS induced intercellular chromatin transmigration in Papaver somniferum L.Short Communication

Girjesh KUMAR, Sana NASEEM
Plants Genetics Laboratory, Department of Botany, University of Allahabad, Allahabad, India

The phenomenon of chromatin migration was observed during microsporogenesis in an ethyl methane sulphonate (EMS) treated population of poppy, which is an important medicinal plant. Cytomixis occurred through a cytoplasmic channel or by direct fusion of pollen mother cells (PMCs); the former was more recurring than the latter. The process was associated with irregular meiosis. PMCs with differing chromosome numbers from the normal diploid number (2n = 22) through cytomixis may lead to the production of aneuploid and polyploid gametes. An increase in the concentration of EMS had a positive effect on the percentage of PMCs showing cytomixis. In addition to cytomixis, other chromosomal abnormalities were also found. Cytomixis along with the related chromosomal abnormalities largely affected the post-meiotic products resulting in some pollen sterility.

Keywords: cytomixis; ethyl methane sulphonate; microsporogenesis; pollen sterility; poppy

Published: June 30, 2013  Show citation

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KUMAR G, NASEEM S. EMS induced intercellular chromatin transmigration in Papaver somniferum L. Czech J. Genet. Plant Breed. 2013;49(2):86-89. doi: 10.17221/85/2012-CJGPB.
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    References

    1. Bahl J.R., Tyagi B.R. (1988): Cytomixis in pollen mother cells of Papaver dubium L. Cytologia, 53: 771-775. Go to original source...
    2. Boldrini K.R., Pagliarini M.S., do Valle C.B. (2006): Cell fusion and cytomixis during microsporogenesis in Brachiaria humidicola (Poaceae). South African Journal of Botany, 72: 478-481. Go to original source...
    3. Falistocco E., Tosti N., Falcinelli M. (1995): Cytomixis in pollen mother cells of diploid Dactylis, one of the origins of 2n gametes. Journal of Heredity, 86: 448-453. Go to original source...
    4. Gates R.R. (1911): Pollen formation in Oenothera gigas. Annals of Botany, 25: 909-940. Go to original source...
    5. Ghaffari S.M. (2006): Occurrence of diploid and polyploidy microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. African Journal of Biotechnology, 5: 1450-1453.
    6. Haroun S.A., Al Shehri A.M., Al Wadie H.M. (2004): Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae). Cytologia, 69: 7-11. Go to original source...
    7. Heslop-Harrison J. (1966): Cytoplasmic connections between angiosperm meiocytes. Annals of Botany, 30: 592-600. Go to original source...
    8. Koernicke M. (1901): Über ortsveränderung von Zellkärnern. S.B. Niederhein, Ges Natur-U Heilkunde Bonn A, 14-25. (in German)
    9. Kumar G., Sharma V. (2002): Induced cytomixis in chick pea (Cicer arietinum L.). Nucleus, 45: 24-26.
    10. Lattoo S.K., Khan S., Bamotra, Dhar A.K. (2006): Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thumb) Jacq. an additional strategy and possible implications. Journal of Biosciences, 31: 629-637. Go to original source... Go to PubMed...
    11. Maheshwari P. (1950): An Introduction to the Embryology of Angiosperms. McGraw Hill, New York. Go to original source...
    12. McLean B.G., Hempel F.D., Zambryski P.C. (1997): Plant intercellular communication via plasmodesmata. Plant Cell, 9: 1043-1054. Go to original source... Go to PubMed...
    13. Mehra P.N., Kalia V. (1973): Accessory chromosomes and multinucleate pollen mother cells in Saccharum benghalense Retz. complex. Nucleus, 16: 75-78.
    14. Miehe H. (1901): Über die Wanderungen des pflanzlichen Zellkernes. Flora, 88: 105-142. (in German)
    15. Price S. (1956): Cytological studies in Saccharum and allied genera 1. Syncytes in certain clones of Saccharum and Erianthus. Cytologia, 21: 21-37. Go to original source...

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