Czech Journal of Genetics and Plant Breeding, 2012 (vol. 48), issue 1
Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expressionReview
Allah Bakhsh, Tayyab Husnain
Czech J. Genet. Plant Breed., 2012, 48(1):1-9 | DOI: 10.17221/34/2011-CJGPB 
Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The successful application of transgenic technology has been further strengthened by the availability of a broad spectrum of promoters having the ability to regulate the temporal and spatial expression patterns of the transgene. A variety of promoters is necessary at all levels of genetic engineering in plants, from basic research discoveries, to development of economically viable crops and plant commodities, to addressing legitimate concerns raised about the...
Effect of the nud gene on grain yield in barleyOriginal Paper
Delfina Barabaschi, Enrico Francia, Alessandro TONDELLI, Alberto GIANINETTI, Antonio M. STANCA, Nicola PECCHIONI
Czech J. Genet. Plant Breed., 2012, 48(1):10-22 | DOI: 10.17221/117/2011-CJGPB
Naked barleys are less yielding than the hulled ones while the reason for this difference has not been definitely clarified. To investigate the effect of the nud gene on yield, a barley doubled haploid (DH, Proctor × Nudinka) population was initially tested in three environments and a QTL study was run on the entire population as well as on two nud/NUD DH subpopulations. Among the agronomic traits studied, a QTL effect was found at nud locus on chromosome 7H only for yield and thousand grain weight (TGW), while a second QTL was found on 6H, although contributed by the naked parent. Other QTLs for TGW were identified on 2H, 3H and...
Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian oasesOriginal Paper
Ines Bellil, Mohammed Chekara Bouziani, Douadi Khelifi
Czech J. Genet. Plant Breed., 2012, 48(1):23-32 | DOI: 10.17221/105/2011-CJGPB
Saharan wheats have been studied particularly from a botanical viewpoint. Genotypic identification, classification and genetic diversity studies to date were essentially based on the morphology of the spike and grain. For this, the allelic variation at the glutenin loci was studied in a set of Saharan bread and durum wheats from Algerian oases where this crop has been traditionally cultivated. The high molecular weight and low molecular weight glutenin subunit composition of 40 Saharan bread and 30 durum wheats was determined by SDS-PAGE. In Saharan bread wheats 32 alleles at the six glutenin loci were detected, which in combination resulted in 36...
Enhanced resistance to Verticillium dahliae in transgenic cotton expressing an endochitinase gene from Phaseolus vulgarisOriginal Paper
Masoud TOHIDFAR, Robabeh HOSSAINI, Nemat SHOKHANDAN BASHIR, Tabatabaei MEISAM
Czech J. Genet. Plant Breed., 2012, 48(1):33-41 | DOI: 10.17221/89/2011-CJGPB
The cotton wilt disease caused by the fungus Verticillium dahliae reduces total yield and quality in cotton worldwide. Since traditional breeding has achieved only low levels of wilt resistance, we used a transgenic approach to produce wilt tolerant cotton. We produced fertile transgenic T2 cotton lines by Agrobacterium-mediated transfer of an endochitinase gene from Phaseolus. Transgene integration and expression were confirmed by PCR amplification, Southern blot and Western blot analyses. Seedlings of homozygous T2 plants with high levels of chitinase expression were evaluated for their tolerance to...
Transgene coding of a key enzyme of the glycolytic pathway helps to decrease sugar content in potato tubersShort Communication
Oldřich NAVRÁTIL, Petr BUCHER, Josef VACEK
Czech J. Genet. Plant Breed., 2012, 48(1):42-45 | DOI: 10.17221/147/2011-CJGPB
Cold-stored potato tubers gradually accumulate reducing sugars. A proposed reason is a cold-induced blocking of glycolysis. The introduction and expression of the bacterial gene Lbpfk coding for cold-tolerant phosphofructokinase might counteract this effect. We have recently introduced this gene into several Czech potato cultivars. The obtained transgenic lines were then tested for three years in field trials. In 17 transgenic lines derived from two of the cultivars we have investigated the accumulation of reducing sugars during two and four months of cold storage. Although in all transgenic lines the sugar content still increased between...
Green pea variety TwinsetNew Varieties
I. Hasalová, R. Trojan, R. Dostálová
Czech J. Genet. Plant Breed., 2012, 48(1):46-47 | DOI: 10.17221/167/2011-CJGPB
List of field crop varieties registered in the Czech Republic in 2011New Varieties
R. Šafaříková
Czech J. Genet. Plant Breed., 2012, 48(1):48-49 | DOI: 10.17221/52/2012-CJGPB