Czech Journal of Genetics and Plant Breeding, 2011 (vol. 47), issue 3

Mapping of QTLs involved in resistence to rice blast (Magnaporthe grisea) using Oryza minuta introgression linesOriginal Paper

Lutfor Rahman, Sakina Khanam, Jae-Hwan Roh, Hee-Jong Koh

Czech J. Genet. Plant Breed., 2011, 47(3):85-94 | DOI: 10.17221/19/2011-CJGPB  

Introgression lines derived from Oryza minuta and O. sativa subsp. japonica var. Junambyeo were crossed for a mapping of the population composed of 112 recombinant lines to identify putative QTLs against rice blast disease using the percentage of diseased leaf area. By using 148 Sequence Tagged Site (STS) and Single Sequence Repeat (SSR) markers, five QTLs on chromosomes 6, 7, 9 and 11 and seven epistatic QTLs were identified against two blast isolates (KI307 and KI209). Of them two QTLs (qKI307-2 and qKI209-3) shared a similar position on chromosome 11. O. minuta introgression contributed the resistance...

Dissimilarity of barley powdery mildew resistances Lomerit and Heils HannaOriginal Paper

Antonín DREISEITL

Czech J. Genet. Plant Breed., 2011, 47(3):95-100 | DOI: 10.17221/45/2011-CJGPB  

The resistance Heils Hanna (HH) was postulated in several tens of 471 previously tested winter barley cultivars. In this paper, new tests on 29 of these cultivars are reported. Thirty-two reference isolates of Blumeria graminis f.sp. hordei held in the pathogen genebank at the Agricultural Research Institute in Kromeriz, Ltd. including a Japanese isolate and five Israeli isolates were used for response tests. However, the resistance HH conferred by the gene Mla8 and herein characterised by reaction type 0 to an old Japanese isolate known as Race I was now postulated only in four cultivars. In the other 25 cultivars...

Dynamic genetic effects on threonine content in rapeseed (Brassica napus L.) meal at different developmental stagesOriginal Paper

Guolin CHEN, Jianguo WU, Chunhai SHI

Czech J. Genet. Plant Breed., 2011, 47(3):101-113 | DOI: 10.17221/17/2011-CJGPB  

Dynamic genetic effects on threonine content (TC) of rapeseed (Brassica napus L.) meal were analysed at 5 developmental times/stages using the genetic models for diploid plant seeds. Results indicated that the expression of diploid embryo, cytoplasmic and diploid maternal plant genes were all important for the performance of TC at various developmental times/stages of rapeseed, especially at the early and middle developmental stages. Among different genetic systems, TC was mainly controlled by the cumulative or net maternal main effects and the genotype × environment (GE) interaction effects, followed by the embryo main effects and GE interaction...

Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L.Original Paper

Samad Habibzadeh ARDEBILI, Mehran E. SHARIATPANAHI, Reza AMIRI, Mona EMAMIFAR, Mahnaz OROOJLOO, Ghorbanali NEMATZADEH, Seyed Ahmad Sadat NOORI, Erwin HEBERLE-BORS

Czech J. Genet. Plant Breed., 2011, 47(3):114-122 | DOI: 10.17221/4/2011-CJGPB  

The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15-45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min)...

Development of a small-scale variant of the Rapid Mix Test experimental bread bakingShort Communication

Tibor SEDLÁČEK, Pavel HORČIČKA

Czech J. Genet. Plant Breed., 2011, 47(3):123-127 | DOI: 10.17221/47/2011-CJGPB  

To determine wheat quality, an experimental 80 g variant (mini-RMT) of the standard 1050 g Rapid Mix Test (RMT) was developed. Ten samples of wheat flour with known RMT values were analysed using the method. Results showed a strong correlation between RMT and mini-RMT values (r = 0.96). Mini-RMT bread volumes were smaller on average by 47.4 ml. A regression equation was constructed allowing the mini-RMT to be used in place of the RMT in wheat breeder laboratories. Anyway, these results are of limited plausibility due to the limited sample numbers and have to be verified on a larger sample set.

Medium-linolenic linseed (Linum usitatissimum L.) RaciolNew Varieties

E. Tejklová, M. Bjelková, M. Pavelek

Czech J. Genet. Plant Breed., 2011, 47(3):128-130 | DOI: 10.17221/96/2011-CJGPB