Czech Journal of Genetics and Plant Breeding, 2010 (vol. 46), issue 2
Proteomics: taking over where genomics leaves offReview
Ritu MAHAJAN, Priyanka GUPTA
Czech J. Genet. Plant Breed., 2010, 46(2):47-53 | DOI: 10.17221/34/2009-CJGPB 
The proteomic studies are simultaneously developed in several directions and significantly influence our notions on the capabilities of biological sciences. The need for proteomics research is necessary as there are certain genes in a cell that encode proteins with specific functions. Using a variety of techniques, proteomics can be used to study how proteins interact within a system or how the protein expression changes in different parts of the body, in different stages of its life cycle and in different environmental conditions as every individual has one genome and many proteomes. Besides the qualitative and quantitative description of the expressed...
Response of durum wheat (Triticum durum Desf.) growth to salt and drought stressesOriginal Paper
Rhouma SAYAR, Houcine BCHINI, Mohamed MOSBAHI, Habib KHEMIRA
Czech J. Genet. Plant Breed., 2010, 46(2):54-63 | DOI: 10.17221/85/2009-CJGPB
Two durum wheat (Triticum durum Desf.) cultivars were tested for salt and drought tolerance at germination, seedling emergence and early seedling growth in NaCl and PEG-8000 solutions of different osmotic potentials (-0.2, -0.4, -0.6 and -0.8 MPa). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, seedling growth, fresh and dry weight were recorded under controlled conditions. Results showed that germination and emergence rates were delayed by both solutions in both cultivars, but Omrabia showed higher germination and emergence rates than BD290273 in NaCl while BD290273 was less affected...
SSR markers linked to mite (Polyphagotarsonemus latus Banks) resistance in jute (Corchorus olitorius L.)Original Paper
Ajit Ghosh, Sazia Sharmin, Sazzadul Islam, Minhaz Uddin Pahloan, Shahidul Islam, Haseena Khan
Czech J. Genet. Plant Breed., 2010, 46(2):64-74 | DOI: 10.17221/71/2009-CJGPB
The mite is considered as one of the major limiting factors of jute production in Bangladesh. So it is essential to select genotypes with the desired resistance allele combination using DNA markers. Eighty-eight SSR primers were used to screen the mite resistant and sensitive parents. Among them only twenty-one primer pairs showed polymorphism. A genetic linkage map was constructed using these polymorphic primers in one hundred and fifty F2 populations, derived from a cross between the parents. The SSR markers J-170 and HK-89 have been mapped at 34.1 cM and 35.4 cM, respectively, on either side of the phenotypic marker. These two markers...
Evaluation of DNA polymorphism among cultivated and wild grapevine accessions from AzerbaijanOriginal Paper
Samira SALAYEVA, Ellada AKHUNDOVA, Alamdar MAMMADOV
Czech J. Genet. Plant Breed., 2010, 46(2):75-84 | DOI: 10.17221/12/2010-CJGPB
To estimate genetic relationships among 31 cultivated and 34 wild grape accessions originating from regions near the Caspian Sea in the Azerbaijan Republic, RAPD analysis was performed with 27 decamer primers selected from a total of 55 primers. The most discriminating primers were OPC-16, OPF-18 and OPA-17, which showed the highest values of genetic diversity (0.927, 0.914 and 0.909, respectively). The lowest values of diversity pertained to the markers OPA-1 (0.615) and V-20 (0.624). The cluster analysis representing genetic similarity among all selected samples divided the genotypes into nine separate groups at similarity index 0.508. Within the...
Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheatShort Communication
Taťána SUMÍKOVÁ, Alena HANZALOVÁ
Czech J. Genet. Plant Breed., 2010, 46(2):85-89 | DOI: 10.17221/32/2010-CJGPB
Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered in the Czech Republic. Gene Lr37 was detected in four tested cultivars (Bakfis, Biscay, Nicol, Mulan), gene Lr26 occurred only in one cultivar (Etela) and three cultivars (Clarus, Orlando and Rapsodia) were found to carry both these genes. Data obtained by PCR markers were compared with results of greenhouse and field tests. Seedling reactions of cultivars possessing Lr26 to seven different leaf rust isolates conformed to the results obtained by the marker...
Rusts epidemics and their implications in wheat breeding and research in the Czech RepublicScientific Reports
P. Bartoš
Czech J. Genet. Plant Breed., 2010, 46(2):90-96 | DOI: 10.17221/52/2010-CJGPB
Winter wheat FedererNew Varieties
P. Laml, J. Pánek
Czech J. Genet. Plant Breed., 2010, 46(2):97-98 | DOI: 10.17221/54/2010-CJGPB
Winter wheat SeceseNew Varieties
P. Horčička, O. Veškrna, T. Sedláček, J. Chrpová
Czech J. Genet. Plant Breed., 2010, 46(2):99-101 | DOI: 10.17221/55/2010-CJGPB