Czech Journal of Genetics and Plant Breeding, 2009 (vol. 45), issue 1
Potato variety identification by molecular markers based on retrotransposon analysesOriginal Paper
Alena Nováková, Kateřina Šimáčková, Jan Bárta, Vladislav Čurn
Czech J. Genet. Plant Breed., 2009, 45(1):1-10 | DOI: 10.17221/11/2008-CJGPB 
We analyzed a set of twenty most grown potato (Solanum tuberosum L.) varieties listed in the Czech Variety List using the PCR-IRAP (Inter-Retrotransposon Amplified Polymorphism) method in order to distinguish fast and unambiguously the varieties. In total, 62 polymorphic alleles were amplified using the three primers P-Tst-1, P-Tst-3 and P-Tst-6. The recorded pattern of markers was stable and reproducible. The analyses were repeated three times and identical results were always obtained. The level of polymorphism varied from 11% to 79% depending on the respective primer. All analysed varieties could be reliably distinguished after multivariate...
Enrichment of chickpea genetic resources collection monitored by microsatellitesOriginal Paper
Klaudia Jomová, Michaela Benková, Ján Kraic
Czech J. Genet. Plant Breed., 2009, 45(1):11-17 | DOI: 10.17221/49/2008-CJGPB
A set of 49 chickpea accessions maintained in the collection of the Genebank of the Slovak Republic was analyzed using microsatellite markers. The level of genetic diversity and the effectivity of new germplasm acquisition for the collection enrichment were evaluated. Five primer pairs used generated 50 different amplified alleles. Four of them, TA2, TA5, TR1, and TR7, containing a long TAA-tandem repeat, were polymorphic with 11-13 alleles per locus. A single fragment was obtained from all the accessions with the primer pair CATPER flanking a small microsatellite repetition in the mRNA sequence of chickpea cationic peroxidase. The genetic diversity...
Yield stability and resistance to leaf spot diseases and rosette in groundnutOriginal Paper
Godfrey Akpan IWO, P.E. OLORUNJU
Czech J. Genet. Plant Breed., 2009, 45(1):18-25 | DOI: 10.17221/30/2008-CJGPB
Twenty-three advanced groundnut lines were evaluated for yield and resistance to early leaf spot (Mycosphaerella arachidis Deighton), late leaf spot (Mycosphaerella berkeleyi W.A. Jenkins) and rosette virus in on-station trials in 2001 and 2002. All the early groundnut lines were relatively resistant to rosette virus, early leaf spot and late leaf spot except ICGV-SM-93523 and ICGV-SM-93525, which were susceptible to late leaf spot. The medium maturing lines showed mostly higher levels of diseases, except MS16-791, which performed very well against all three diseases. The late groundnut lines were mostly susceptible to one or more...
Non-hypersensitive leaf rust resistance of bread wheat cultivar PBW65 conditioned by genes different fromLr34Short Communication
Mohammad Anwar KHAN, Ram Gopal SAINI
Czech J. Genet. Plant Breed., 2009, 45(1):26-30 | DOI: 10.17221/51/2008-CJGPB
: The bread wheat (Triticum aestivum L.) cultivar PBW65 has shown hight levels of resistance to the most frequent and highly virulent Indian race 77-5 of leaf rust (Puccinia triticina). The infection type and disease severity indicated a non-hypersensitive type of resistance against the race 77-5 in PBW65. The cultivar PBW65 was crossed with the leaf rust susceptible cultivar WL711 to determine the mode of inheritance of the resistance. The segregation for resistant and susceptible plants in the F2 and F3 generations revealed, that two genes, each showing additive effects, were likely to confer resistance to...
IV International conference on legume genomics and genetics. Puerto Vallarta, Mexico, December 7-12, 2008Conference Report
P. Smýkal
Czech J. Genet. Plant Breed., 2009, 45(1):31-34 | DOI: 10.17221/2661-CJGPB
Poppy variety OrfeusNew Varieties
V. Vrbovský
Czech J. Genet. Plant Breed., 2009, 45(1):35-36 | DOI: 10.17221/3/2009-CJGPB
White-seeded poppy varieties Orel and RacekNew Varieties
L. Kameníková, V. Vrbovský
Czech J. Genet. Plant Breed., 2009, 45(1):37-38 | DOI: 10.17221/5/2009-CJGPB