Czech Journal of Genetics and Plant Breeding, 2008 (vol. 44), issue 2

Assessing resistance to head blight in wheat cultivars inoculated with different Fusarium isolatesOriginal Paper

Václav Šíp, Jana Chrpová, Světlana Sýkorová

Czech J. Genet. Plant Breed., 2008, 44(2):43-59 | DOI: 10.17221/19/2008-CJGPB  

Four winter wheat cultivars, differing in resistance to Fusarium head blight (FHB), were spray inoculated under field conditions for three years with 18 isolates of two Fusarium species (F. graminearum and F. culmorum) obtained from different regions of the Czech Republic. Deoxynivalenol (DON) contamination, FHB severity (VSS), Fusarium damaged kernels (FDK) and reductions of thousand grain weight and grain weight per spike were measured to describe the aggressiveness level of isolates. Highly significant correlations were found between these traits, but correlation coefficients ranged from 0.45 to 0.99. Analyses of variance...

Crown rust pathotypes determined on oats in the Czech Republic from 2004 to 2006 and reaction to oat cultivarsOriginal Paper

Hana Jiráková, Alena Hanzalová

Czech J. Genet. Plant Breed., 2008, 44(2):60-65 | DOI: 10.17221/15/2008-CJGPB  

The incidence of oat crown rust (Puccinia coronata var. avenae Fraser et Ledingham) pathotypes was recorded in the Czech Republic in 2004-2006. The virulence of collected 79 monopustule oat crown rust isolates was assessed on 23 differentials. High diversity was observed; the isolates possessed from 0 to 8 virulence genes. More than 80% of pathotypes were recorded only once. Pathotypes BLBG and BLBC were the most frequent. None of the tested isolates was virulent to resistance genes Pc39, Pc50, Pc52, Pc59, Pc62 and Pc68. Registered cultivars Avenuda, Dalimil, Auron, Isak were susceptible to the majority of pathotypes,...

Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagationOriginal Paper

Lenka Klčová, Marcela Gubišová

Czech J. Genet. Plant Breed., 2008, 44(2):66-72 | DOI: 10.17221/2677-CJGPB  

Plant regeneration by different techniques was evaluated in three buckwheat cultivars: indirect regeneration from cotyledons and hypocotyls, direct regeneration by nodal segment cultivation and induction of multiple shoots from seedling apices. Regenerated shoots were obtained in all procedures. The effects of BA (6-benzylaminopurine), media composition and gelling agent were tested. The regeneration efficiency of shoot apex culture was 2.65-3.33 nodal segments/explant. Cotyledon and hypocotyl segments produced 1.25-2.44 shoots per explant plated. Nodal segment cultivation yielded 4.1-4.8 new nodal segments/explant in 4 weeks. Eighty percent of shoots...

Effect of inoculum doses on common bunt infection on wheat caused by Tilletia tritici and T. laevisShort Communication

Veronika DUMALASOVÁ, Pavel BARTOŠ

Czech J. Genet. Plant Breed., 2008, 44(2):73-77 | DOI: 10.17221/2/2008-CJGPB  

: In the trial with different doses of common bunt teliospores used for inoculation of wheat the number of bunted ears increased with the increasing dose of inoculum. This increase was small (statistically insignificant) in the resistant cv. Bill and high (statistically significant) in the susceptible cv. Samanta. The effect of inoculation on the vigour of inoculated plants was estimated according to the number of tillers per plot in summer in cultivars inoculated with different inoculum doses sown at the same sowing rate in autumn. The number of tillers was decreasing with the increasing inoculum dose. This was less pronounced in the resistant cv....

International conference: Molecular mapping and marker assisted selection in plants. Vienna 3rd-6th February 2008Conference Report

P. Smýkal

Czech J. Genet. Plant Breed., 2008, 44(2):78-79 | DOI: 10.17221/3143-CJGPB  

Winter wheat SultanNew Varieties

P. Horčička, A. Hanišová, O. Veškrna

Czech J. Genet. Plant Breed., 2008, 44(2):81-82 | DOI: 10.17221/29/2008-CJGPB