Czech Journal of Genetics and Plant Breeding, 2006 (vol. 42), issue 3
Breeding Barley for Multiple Disease Resistance in the Upper MidwestRegion of the USA
Brian J. Steffenson, Kevin P. Smith
Czech J. Genet. Plant Breed., 2006, 42(3):79-86 | DOI: 10.17221/3646-CJGPB 
Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs
Ulrich Kurt Posselt, Philippe Barre, Gintaras Brazauskas, Lesley Beryl Turner
Czech J. Genet. Plant Breed., 2006, 42(3):87-94 | DOI: 10.17221/3647-CJGPB
Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for...
The Potential of Common Cereals to form Retrograded Resistant Starch
Daniela Mikulíková, Michaela Benková, Ján Kraic
Czech J. Genet. Plant Breed., 2006, 42(3):95-102 | DOI: 10.17221/3648-CJGPB
Resistant starch (RS) has been recognised as a functional fibre with many health-promoting effects. RS exists in four forms - RS1, RS2, RS3, and RS4. The RS3 type is generated by amylose retrogradation typically resulting from food processing procedures. The aim of this study was to demonstrate the potential ability of six agriculturally important cereals to generate type RS3 resistant starch after retrogradation of their amylose. In comparison with all tested cereals, the statistically significant highest level of RS3 (5.28% ± 0.68) was detected in triticale, mainly...
A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.
Narpal Deep Kaur, Miroslava Vyvadilová, Miroslav Klíma, Miroslav Bechyně
Czech J. Genet. Plant Breed., 2006, 42(3):103-110 | DOI: 10.17221/3649-CJGPB
An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8-11.2 × 104 protoplasts/ml in darkness at 25°C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures...
Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction
Jozef Gubiš, Martina Hudcovicová, Marcela Gubišová
Czech J. Genet. Plant Breed., 2006, 42(3):111-114 | DOI: 10.17221/3650-CJGPB
PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed. For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was used. Amounts from 1.8 to 419.1 pg of R. secalis DNA per 100 ng of total DNA were detected in 18 samples of barley seeds contaminated by R. secalis in field conditions. The correctness of this quantitative analysis was checked using an artificial infection of seeds with 1, 2, 5 and 20% level of infection by R. secalis. The level of contamination of artificially infected samples decreased with a lowering amount of added seed...
Winter Oilseed Rape Oponent
R. Koprna
Czech J. Genet. Plant Breed., 2006, 42(3):115-116 | DOI: 10.17221/6060-CJGPB