Czech J. Genet. Plant Breed., 2006, 42(3):87-94 | DOI: 10.17221/3647-CJGPB

Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

Ulrich Kurt Posselt1, Philippe Barre2, Gintaras Brazauskas3, Lesley Beryl Turner4
1 State Plant Breeding Institute, University of Hohenheim, Stuttgart, Germany
2 National Institute of Agronomic Research (INRA), Forage Genetics and Breeding Unit (UGAPF), Lusignan, France
3 Lithuanian Institute of Agriculture, Akademija, Kédainiai, Lithuania
4 Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, United Kingdom

Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).

Keywords: marker comparison; AFLP; ISSR; RAPD; SSR; Lolium perenne L.

Published: September 30, 2006  Show citation

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Posselt UK, Barre P, Brazauskas G, Turner LB. Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs. Czech J. Genet. Plant Breed. 2006;42(3):87-94. doi: 10.17221/3647-CJGPB.
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