Czech Journal of Genetics and Plant Breeding - Online first
Genetic diversity analysis of Solanum accessions from Czech collections of potato genetic resources using nuclear SSR markersOriginal Paper
Zuzana Rottová, Tereza Anna Javůrková, Petr Sedlák, Jiří Ptáček, Eloy Fernández-Cusimamani, Vladimíra Sedláková
Czech J. Genet. Plant Breed., X:X 
The genus Solanum comprises numerous wild and cultivated species that are important for potato breeding. This pilot-scale study aimed to evaluate the genetic diversity in 44 accessions from Solanum sect. Petota, comprising wild species, Andean landraces, and modern cultivars, obtained from the Potato Research Institute Havlíčkův Brod, Ltd. and the Department of Crop Sciences and Agroforestry at the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague. Nuclear microsatellite markers (SSR, 29 loci) were applied via five multiplex PCR reactions and analysed using capillary electrophoresis. Binary data matrices were...
Evaluation of gamma-irradiated Pisum sativum germplasm for agronomic traits and tolerance to Didymella pinodesOriginal Paper
Efi Sarri, Anastasios Katsileros, Sofia Migardou, Panagiotis Viliotis, Ioannis Sidiropoulos, Dimitris Sifnaios, Pavlos Diamantis, Nikolaos Sklavounos, Eleni M. Abraham, Penelope J. Bebeli, Nasya Tomlekova, Dimosthenis Kizis, Eleni Tani
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/84/2025-CJGPB
Ascochyta blight, caused by a complex of pathogenic fungi including Didymella pinodes, Ascochyta pisi, and Phoma pinodella, is a major disease of field pea (Pisum sativum), causing severe losses through lesions on leaves, stems, and pods. Mutation breeding using gamma irradiation is a non-GMO strategy to induce genetic variation and accelerate the development of improved genotypes. In this study, the M2 generation of the forage pea cultivar Dodoni (Pisum sativum L. var. arvense), derived from M0 seeds irradiated with 100 Gy, was evaluated for tolerance to D. pinodes (CBS 251.47)...
Microsatellite markers as a useful tool for species identification and assessment of genetic diversity of the Tilia species in the Czech RepublicOriginal Paper
Pavlína Máchová, Helena Cvrčková, Olga Trčková, Kateřina Vítová, Johana Pechačová, Václav Buriánek, Petr Maděra, Kateřina Houšková, Tomáš Vyhnánek, Pavel Hanáček
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/102/2025-CJGPB
Diagnostic morphological traits distinguishing Tilia cordata and T. platyphyllos are not always apparent. Precise species identification is crucial for conservation management and for establishing genetic resources of forest reproductive material. In this study, we employed fifteen microsatellite (SSR) markers to identify diagnostic loci for reliable species discrimination between T. cordata and T. platyphyllos species and to evaluate the genetic diversity. A total of 250 trees were sampled from eight natural autochthonous populations of T. cordata and T. platyphyllos in the Czech Republic, and additional...
Early activation of CIPK3 in the cold-tolerant potato Solanum commersoniiShort Communication
Roberta Smimmo, Grazia Buonfantino, Clizia Villano, Domenico Carputo, Vincenzo D'Amelia, Riccardo Aversano
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/51/2025-CJGPB
Solanum commersonii shows tolerance to low temperatures, a key target trait for potato breeding. Calcium-dependent protein kinases (CIPKs) play a significant role in plant defence response to several stresses, including cold. In this study, we observed the expression of ScCIPK1, ScCIPK3, ScCIPK23 and ScCIPK24 in S. commersonii exposed to 4 °C at multiple time intervals. Initial findings revealed that these genes were under-expressed after 10 and 30 minutes of cold stress, except ScCIPK3. Notably, after 24 hours, all genes displayed higher expression levels compared to the non-stressed controls. These...