Czech Journal of Genetics and Plant Breeding - Online first
The 90th Birthday of RNDr. Erik Schwarzbach, Dr. agr. habil. (*6 April 1936)Biographical Notice
Antonín Dreiseitl, Věra Kroftová
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/31/2026-CJGPB 
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Whole genome sequencing analysis of two sinensis tea (Camellia sinensis var. sinensis) clones: Assessment of molecular variations to search for breeding markersOriginal Paper
Dwinita Wikan Utami, Adhityo Wicaksono, M. Khais Prayoga, Heri Syahrian, Vitria P. Rahadi, Erdiansyah Rezamela, Budi Martono, Tri Joko Santoso, Nur Kholilatul Izzah, Haris Maulana, Ady Daryanto, Rerenstradika Tizar Terryana, Imas Rita Saadah, David Virya Chen
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/116/2025-CJGPB
Tea (Camellia sinensis (L.) Kuntze) is a globally important crop valued for its flavour diversity and health benefits. Whole-genome sequencing (WGS) was performed to compare genomic variation and functional potential between clone Yabukita and locally adapted clone I.1.93. Using next-generation sequencing, approximately 10× genome coverage was achieved for both clones, with high mapping efficiency (98.24% for Yabukita and 97.88% for clone I.1.93), ensuring reliable downstream analyses. Single nucleotide polymorphism (SNP) analysis revealed distinct genomic patterns, with Yabukita showing a more uniform chromosomal SNP distribution, while clone...
The AhDREB transgene expression activates NtP5CS and NtSUSY, promoting osmotic adjustment in transgenic tobacco under salt stressOriginal Paper
Thuy Thi Xuan Vi, Dang Xuan Hoang, Tra Thi Nguyen, Ngoc Bich Pham, Quan Huu Nguyen, Mau Hoang Chu
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/124/2025-CJGPB
Soil salinity is a major environmental constraint that limits the growth and productivity of peanut (Arachis hypogaea L.), a legume adapted to mildly acidic soils but highly sensitive to saline–alkaline conditions. Dehydration-Responsive Element Binding (DREB) transcription factors are key regulators of plant responses to abiotic stresses. In this study, the AhDREB gene from peanut was introduced into tobacco (Nicotiana tabacum) to examine its functional role under salt stress. The transgenic lines (L32.2 and L37.2) exhibited strong induction of AhDREB expression upon exposure to 150 and 250 mM NaCl, with transcript...
Seasonal and microclimate-responsive expression of VRN-A1 and VRN-B1 in wheat under field conditionsOriginal Paper
Nicole Frantová, Ilja Tom Prášil, Ludmila Holková
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/76/2025-CJGPB
The need of vernalisation, controlled by the gene VRN-1, impacts wheat adaptation and yield stability, yet field evidence on the plasticity of VRN-1 homoeologs expression is limited. We quantified VRN-1 homoeolog dynamics across two sites and two seasons in seven cultivars, by sampling their apex and leaf. VRN-A1 varied with genotype (P < 0.001***), tissue (apex > leaf; P < 0.001***), apex development (P < 0.001***), day length (P < 0.001***), and to a lesser extent, on short-term freezing exposure, quantified as a 5-day freezing-degree sum (FDS; P = 0.019*). Photoperiod...
Evaluating the discriminatory ability and informativeness of DArTseq markers in a comprehensive set of contemporary European potato varietiesOriginal Paper
Petr Sedlák, Vladimíra Sedláková, Jakub Vašek, Ervín Hausvater, Daniela Čílová, Martina Melounová, Jiří Ptáček, Petr Doležal
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/103/2025-CJGPB
High-throughput molecular technology DArTseq generates markers for potential use in molecular breeding of crops. Using DArTseq, we analysed a comprehensive set of 333 European potato varieties reflecting the outcomes of long-term breeding history and representing a potential germplasm for future breeding of potatoes in the Central European region. The varieties were classified according to four factors: region of origin, breeder, earliness and utilisation mode, that may potentially reflect their genetic structure, and for which complete data were publicly available. The DArTseq analysis was performed by the service centre, the Diversity Array...