Czech J. Genet. Plant Breed., 2023, 59(1):43-53 | DOI: 10.17221/49/2022-CJGPB

Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in riceOriginal Paper

Yu Li*,1, Xiaomi Chen1, Tao Lan1, Jing Zhang2, Ziheng Chen2, Wenting Yang3, Xinmei Lin3
1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, P.R. China
2 College of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, P.R. China
3 Jinshan College, Fujian Agriculture and Forestry University, Fuzhou, P.R. China

Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC1F1) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a restoring line of CMS-FA hybrid rice, we obtained the genomic sequence of the mapping region, which provided the basis for the prediction of the candidate gene(s) of the target gene and for the comparison of genomic sequence differences between wild and cultivated rice. Within the mapping region, the genomic sequence of the wild rice was significantly different from that of cultivated rice. There were ten genes in the final mapping region. A pentatricopeptide repeat (PPR) protein gene was predicted as the candidate gene of Rf(fa). Our results laid a solid foundation for the final cloning and molecular mechanism analysis of the gene. The identified molecular markers tightly linked to Rf(fa) will facilitate the marker assisted selection in breeding of CMS-FA hybrid rice.

Keywords: fertility restorer; gene fine mapping; prediction of candidate gene(s); rice

Received: June 20, 2022; Accepted: September 9, 2022; Prepublished online: October 10, 2022; Published: December 14, 2022  Show citation

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Li Y, Chen X, Lan T, Zhang J, Chen Z, Yang W, Lin X. Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice. Czech J. Genet. Plant Breed. 2023;59(1):43-53. doi: 10.17221/49/2022-CJGPB.
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