Czech J. Genet. Plant Breed., 2021, 57(1):19-25 | DOI: 10.17221/47/2020-CJGPB

Combining the null Kunitz trypsin inhibitor and yellow mosaic disease resistance in soybean (Glycine max (L.) Merrill)Original Paper

Sandeep Kaur Dhaliwal ORCID...*,1, Satwinder Kaur Dhillon1, B.S. Gill1, Asmita Sirari1, Anita Rani2, Roopan Dhillon1
1 Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India
2 ICAR - Indian Institute of Soybean Research, Indore, Madhya Pradesh, India

For the wide adoption of soybean varieties, it is desirable, that they are Kunitz trypsin inhibitor (Kti) free and resistant to yellow mosaic disease (YMD). The soybean variety SL525 with YMD resistance was crossed with the YMD susceptible variety NRC101 with a null kti allele. The F5 progeny derived from the cross was screened with two simple sequence repeat (SSR) markers (satt409 and satt322) linked with the null kti allele and the YMD resistance, respectively, and one null kti allele-specific marker. The presence of both desirable traits was further confirmed with the phenotypic data which showed good correlation with the genotypic data. The yield potential of fourteen such identified genotypes having both desirable traits was either at par or superior to SL525, hence, represent improved versions of SL 525.

Keywords: marker assisted selection; null kti allele; SSR markers; YMD

Published: January 7, 2021  Show citation

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Dhaliwal SK, Dhillon SK, Gill BS, Sirari A, Rani A, Dhillon R. Combining the null Kunitz trypsin inhibitor and yellow mosaic disease resistance in soybean (Glycine max (L.) Merrill). Czech J. Genet. Plant Breed. 2021;57(1):19-25. doi: 10.17221/47/2020-CJGPB.
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    References

    1. Cregan P.B., Jarvik T., Bush A.L., Shoemaker R.C., Lark K.G., Kahler A.L., Kaya N., VanToai T.T., Lohnes D.G., Chung J., Specht J.E. (1999): An integrated genetic linkage map of the soybean genome. Crop Science, 39: 1464-1490. Go to original source...
    2. Doyle J.J., Doyle J.L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Go to original source...
    3. Hildebrand D.F., Orf J.H., Hymowitz T. (1980): Inheritance of an acid phosphatase and its linkage with the Kunitz trypsin inhibitor in seed protein of soybeans. Crop Science, 20: 83-85. Go to original source...
    4. Jofuku K.D., Goldberg R.B. (1989): Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants. Plant Cell, 1: 1079-1093. Go to original source...
    5. Kakade M.L., Rackis J.J., McGhee J.E., Puskin G. (1974): Determination of trypsin inhibitor of soy products, a collaborative analysis of an improved procedure. Cereal Chemistry, 51: 376-382.
    6. Khosla G. (2018): Genetic and molecular assessment of breeding methods and mapping novel QTL in soybean (Glycine max L. Merrill). [Ph.D. Thesis.] Ludhiana, Punjab Agricultural University. Available at http://krishikosh.egranth.ac.in/handle/1/5810073947
    7. Kiang Y.T. (1987): Mapping three protein loci on a soybean chromosome. Crop Science, 27: 44-46. Go to original source...
    8. Kim M.S., Park M.J., Jeong W.H., Nam K.C., Chung J.I. (2006): SSR marker tightly linked to Ti locus in soybean. Korean Journal of Crop Science, 49: 419-422.
    9. Kunitz M. (1945): Crystallization of a soybean trypsin inhibitor from soybean. Science, 101: 668-669. Go to original source... Go to PubMed...
    10. Kumar B., Talukdar A., Verma K., Bala I., Harish G.D., Gowda S., Lal S.K., Sapra R.L., Singh K.P. (2015): Mapping of yellow mosaic disease (YMD) resistance in soybean (Glycine max L. Merril.) through association mapping approach. Genetica, 143: 1-10. Go to original source... Go to PubMed...
    11. Kumar K.V. (2019): Molecular characterization of introgression for YMV in backcross derivatives of soybean cultivar, JS335. [Master's Thesis.] Ludhiana, Punjab Agricultural University. Available at http://krishikosh.egranth.ac.in/handle/1/5810127472
    12. Lazarowitz S., Shepherd R.J. (1992): Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences, 11: 327-349. Go to original source...
    13. Moraes De R.M.A., Soares T.C.B., Colombo L.R., Salla M.F.S., Barros J.G., Piovesan N., Moreira M.A. (2006): Assisted selection by specific DNA markers for genetic elimination of the Kunitz trypsin inhibitor and lectin in soybean seeds, Euphytica, 149: 221-226. Go to original source...
    14. Nene Y.L. (1973): Viral diseases of some warm weather pulse crops in India. Plant Disease Reporter, 57: 463-467.
    15. Orf J.H., Hymowitz T. (1979): Genetics of the Kunitz trypsin inhibitor, an antinutritional factor in soybeans. Journal of the American Chemical Society, 56: 722-726. Go to original source...
    16. Qazi J., Ilyas M., Mansoor S., Briddon R.W. (2007): Legume yellow mosaic viruses: genetically isolated begomoviruses. Molecular Plant Pathology, 8: 343-348. Go to original source... Go to PubMed...
    17. Rani A., Kumar V., Husain S.M., Chauhan G.M. (2010): NRC101 (IC0582901; IN GR10054) and NRC102 (IC582902; INGR10055): Soybean (Glycine max) germplasm lines free from Kunitz inhibitor polypeptide. Indian Journal of Plant Genetic Research, 24: 122.
    18. Rani A., Kumar V., Mourya V., Singh R.K., Husain S.M. (2011): Validation of SSR markers linked to null Kunitz trypsin inhibitor allele in Indian Soybean (Glycine max (L.) Merril) population. Journal of Plant Biochemistry and Biotechnology, 20: 258-261. Go to original source...
    19. Rani A., Kumar V., Gill B.S., Rathi P., Shukla S., Singh R.K., Husain S.M. (2017): Linkage mapping of Mungbean yellow mosaic India virus (MYMIV) resistance gene in soybean. Breeding Science, 67: 95-100. Go to original source... Go to PubMed...
    20. Sadasivam S., Manickam A. (1996): Phenolics. In: Biochemical Methods for Agricultural Sciences. Delhi, Wiley Eastern Ltd.: 187-188.
    21. Sharma A.N., Gupta G.K., Verma R.K., Sharma O.P., Bhagat S., Amaresan N., Saini M.R., Chattopadhayay C., Sushil S.N., Asre R. et al. (2014): Integrated Pest Management for Soybean. Available at http://www.ncipm.org.in).
    22. Singh G., Bhan L.K. (1998): Diseases of mungbean and urdbean and their management. In: IPM System in Agriculture, Pulses, 4: 311-307.
    23. Usharani K.S., Surendranath B., Haq Q.M., Malathi V.G. (2004): Yellow mosaic virus infecting soybean in northern India is distinct from the species infecting soybean in southern and western India. Current Science, 86: 845-850.

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