Czech J. Genet. Plant Breed., 2016, 52(4):157-162 | DOI: 10.17221/16/2016-CJGPB

Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environmentsOriginal Paper

Nadjat BENKHERBACHE1, Alessandro TONDELLI2, Abdelhamid DJEKOUNE3, Enrico FRANCIA4, Nicola PECCHIONI5, Liamine HASSOUS6, Antonio Michele STANCA2
1 Departement of Agricultural Sciences, University of Mohamed Boudiaf - M'sila, M'sila, Algeria
2 Genomics Research Centre, Fiorenzuola d'Arda, Italy
3 Departement of Biology, University of Constantine, Constantine, Algeria
4 University of Reggio Emilia, Modena, Italy
5 National Institute of Cereal Genetic, Foggia, Italy
6 Technical Institute for Field Crops (ITGC), Sétif, Algeria$4

For the purpose of marker assisted selection (MAS), six Algerian cultivars and landraces developed locally or introduced from external breeding programs for semi-arid environments were marker characterized at VRN-H1, VRN-H2 and FR-H2 loci. As controls the lines Nure and Tremois were used. Markers available in the literature, known to discriminate efficiently the trait-associated alleles between the two cultivars Nure and Tremois, were used. This study revealed that the used Algerian cultivars carry a dominant (spring) allele for VRN-H1, while the HvZCCT marker generated a polymorphic profile for the second vernalization locus VRN-H2. No cultivars possessed the Nure allele conferring tolerance to low-temperatures at the HvCBF4. Three cultivars possessed the Tremois allele at FR-H2, while the remaining three presented different haplotypes.

Keywords: Algerian cultivars; growth habit; low-temperature; MAS; VRN-H1; VRN-H2; FR-H2

Published: December 31, 2016  Show citation

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BENKHERBACHE N, TONDELLI A, DJEKOUNE A, FRANCIA E, PECCHIONI N, HASSOUS L, STANCA AM. Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environments. Czech J. Genet. Plant Breed. 2016;52(4):157-162. doi: 10.17221/16/2016-CJGPB.
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