Czech J. Genet. Plant Breed., 2016, 52(4):145-156 | DOI: 10.17221/102/2016-CJGPB

Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia.Original Paper

Farnaz ABED-ASHTIANI1, Jugah KADIR1, Abbas NASEHI1, Seyed-Reza HASHEMIAN-RAHAGHI1, Ganesan VADAMALAI1, 2, Siti-Khadijah RAMBE3
1 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
2 Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3 Laboratory of Bioresource Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) includes important destructive pathogens causing blast disease on various species from the family Poaceae. Thirty-five Magnaporthe isolates were collected from diseased rice plants (variety MR219) in different rice-growing regions of Malaysia, including the five states of Selangor, Penang, Kedah, Kelantan, and Perak between 2010 and 2014. DNA sequence analyses of the internal transcribed spacer (ITS), actin, β-tubulin and calmodulin gene regions, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses were conducted to analyse 35 Magnaporthe isolates. Phylogenetic analysis of the combined dataset confirmed the identification of all isolates as M. oryzae with a high distance from other Magnaporthe (Pyricularia) species. RAPD and ISSR analyses indicated the existence of a relatively low similarity index value among M. oryzae isolates through identification of four main clades. The clustering of RAPD and ISSR analyses demonstrated that there was a correlation between the isolates and their geographical origins.

Keywords: genetic diversity; ISSR; Oryza sativa; RAPD; rDNA-ITS; rice blast

Published: December 31, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
ABED-ASHTIANI F, KADIR J, NASEHI A, HASHEMIAN-RAHAGHI S, VADAMALAI G, RAMBE S. Characterisation of Magnaporthe oryzae isolates from rice in peninsular Malaysia. Czech J. Genet. Plant Breed. 2016;52(4):145-156. doi: 10.17221/102/2016-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abed-Ashtiani F., Kadir J., Selamat A., Mohd-Hanif A.H., Nasehi A. (2012): Effect of foliar and root application of silicon against rice blast fungus in MR219 rice variety. The Plant Pathology Journal, 28: 164-171. Go to original source...
    2. Carbone I., Kohn L.M. (1999): A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia, 91: 553-556. Go to original source...
    3. Chadha S., Gopalakrishna T. (2005): Genetic diversity of Indian isolates of rice blast pathogen (Magnaporthe grisea) using molecular markers. Current Science, 88: 1466-1469.
    4. Chadha S., Gopalakrishna T. (2007): Comparative assessment of REMAP and ISSR marker assays for genetic polymorphism studies in Magnaporthe grisea. Current Science, 93: 688-692.
    5. Consolo V.F., Cordo C.A., Salerno G.L. (2008): DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina. Australasian Plant Pathology, 37: 357-364. Go to original source...
    6. Factfish (2015): Available at http://www.factfish.com/statistic/rice%2C%20paddy%2C%20production%20quantity; http://www.factfish.com/statisticcountry/malaysia/rice%2C%20paddy%2C%20area%20harvested
    7. Glass N.L., Donaldson G.C. (1995): Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61: 1323-1330. Go to original source... Go to PubMed...
    8. Gonzalez-Mendoza D., Argumedo-Delira R., Morales-Trejo A., Pulido-Herrera A., Cervantes-Diaz L., GrimaldoJuarez O., Alarcon A. (2010): A rapid method for isolation of total DNA from pathogenic filamentous plant fungi. Genetics and Molecular Research, 9: 162-166. Go to original source... Go to PubMed...
    9. Hall T.A. (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
    10. Hashim M.M., Yusop M.K., Othman R., Wahid S.A. (2015): Characterization of nitrogen uptake pattern in Malaysian rice MR219 at different growth stages using 15N isotope. Rice Science, 22: 250-254. Go to original source...
    11. Hirata K., Kusaba M., Chuma I., Osue J., Nakayashiki H., Mayama S.H., Tosa Y. (2007): Speciation in Pyricularia inferred from multilocus phylogenetic analysis. Mycological Research, 111: 799-808. Go to original source... Go to PubMed...
    12. Javan-Nikkhah M., McDonald B., Banke S., Hedjaroude G.H. (2004): Genetic structure of Iranian Pyricularia grisea populations based on rep-PCR fingerprinting. European Journal of Plant Pathology, 110: 909-919. Go to original source...
    13. McDonald B.A., Linde C. (2002): Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review of Phytopathology, 40: 349-379. Go to original source... Go to PubMed...
    14. Milgroom M.G., Peever T.L. (2003): Population biology of plant pathogens. The synthesis of plant disease epidemiology and population genetics. Plant Disease, 87: 608-617. Go to original source... Go to PubMed...
    15. Mishra D., Singh U.D., Dash A.B., Reddy J.N., Sridhar R. (2006): Analysis of Pyricularia grisea populations from three different blast epidemics. International Rice Research Newsletter, 31: 22-24. Go to original source...
    16. Mitchell J.L., Roberts P.J., Moss S.T. (1995): Sequence or structure? A short review on the application of nucleic acid sequence information to fungal taxonomy. Mycologist, 9: 67-75. Go to original source...
    17. Nasehi A., Kadir J., Nasr-Esfahani M., Abed-Ashtiani F., Mui-Yun W., Rambe S.K.H., Golkhandan E. (2014): Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops. European Journal of Plant Pathology, 140: 261-273. Go to original source...
    18. Ngueko R.B., Ying S., Hong-kai W., Fu-cheng L., Tong X. (2004): Genetic diversity analysis of Magnaporthe grisea from some blast nurseries of Hunan province using random amplified polymorphic DNA. Journal of Zhejiang University, 30: 355-362.
    19. Park S.Y., Milgroom M.G., Han S.S., Kang S., Lee Y.H. (2003): Diversity of pathotypes and DNA fingerprint haplotypes in populations of Magnaporthe grisea in Korea over two decades. Phytopathology, 93: 1378-1385. Go to original source... Go to PubMed...
    20. Ricker A.J., Ricker R.S. (1936): Introduction to Research on Plant Diseases. St Louis, John Swift Co.
    21. Rohlf E.J. (1993): NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 1.80. Setauket, Applied Biostatistics, Inc.
    22. Scheuermann K.K., Raimondi J.V., Marschalek R., Andrade A., Wickert E. (2012): Magnaporthe oryzae genetic diversity and its outcomes on the search for durable resistance. In: Caliskan M. (ed): The Molecular Basis of Plant Genetic Diversity, Chapter 15. Rijeka, InTech.
    23. Schlotterer C. (2004): The evolution of molecular markers - just a matter of fashion? Nature, 5: 63-69. Go to original source... Go to PubMed...
    24. Séré Y., Onasanya A., Afolabi A., Mignouna H.D., Akator K. (2007): Genetic diversity of the blast fungus, Magnaporthe grisea (Hebert) Barr, in Burkina Faso. African Journal of Biotechnology, 6: 2568-2577. Go to original source...
    25. Sirithunya P., Sreewongchai T., Sriprakhon S., Toojinda T., Pimpisithavorn S., Kosawang C., Smitamana P. (2008): Assessment of genetic diversity in Thai isolates of Pyricularia grisea by Random Amplification of Polymorphic DNA. Journal of Phytopathology, 156: 196-204. Go to original source...
    26. Srivastava D., Shamim M.D., Kumar D., Pandey P., Khan N.A., Singh K.N. (2014): Morphological and molecular characterization of Pyricularia oryzae causing blast disease in rice (Oryza sativa) from north India. International Journal of Scientific and Research Publications, 4: 1-9.
    27. Steenkamp E.T., Wingfield B.D., Desjardins A.E., Marasas W.F.O., Wingfield M.J. (2002): Cryptic speciation in Fusarium subglutinans. Mycologia, 94: 1032-1043. Go to original source...
    28. Swofford D.L. (2002): PAUP*: Phylogenetic Analysis Using Parsimony (and Other Methods), Version 4.0b 10. Sunderland, Sinauer Associates, Inc.
    29. Taylor J.W., Jacobson D.J., Kroken S., Kasuga T., Geiser D.M., Hibbett D.S., Fisher M.C. (2000): Phylogenetic species recognition and species concepts in fungi. Fungal Genetics and Biology, 31: 21-32. Go to original source... Go to PubMed...
    30. Thompson J.D., Higgins D.G., Gibson T.J. (1994): CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-4680. Go to original source... Go to PubMed...
    31. Vanaraj P., Kandasamy S., Ambalavanan S., Ramalingam R., Sabariyappan R. (2013): Variability in Pyricularia oryzae from different rice growing regions of Tamil Nadu, India. African Journal of Microbiology Research, 7: 3379-3388. Go to original source...
    32. White T.J., Bruns T., Lee S., Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A., Geldfand D.H., Sninsky J.J., White T.J. (eds): PCR Protocols: A Guide to Methods and Applications. New York, Academic Press: 315-322. Go to original source...
    33. Zellerhoff N., Jarosch B., Groenewald J., Crous P., Schaffrath U. (2006): Nonhost resistance of barley is successfully manifested against Magnaporthe grisea and a closely related Pennisetum-infected lineage but is overcome by Magnaporthe oryzae. Molecular Plant-microbe Interactions, 19: 1014-1022. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content