Czech J. Genet. Plant Breed., 2014, 50(4):262-267 | DOI: 10.17221/164/2014-CJGPB

Genetic analysis and molecular mapping of leaf rust resistance genes in the wheat line 5R618Original Paper

Jiazhen WANG, Lingzhi SHI, Lin ZHU, Xing LI, Daqun LIU
College of Plant Protection, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, P.R. China

The wheat (Triticum aestivum L.) line 5R618, bred at the China Agricultural University, is resistant in the seedling stage to the majority of the current Chinese pathotypes of wheat leaf rust (Puccinia triticina). To identify and map the leaf rust resistance gene in the 5R618 line, F2 plants and F2:3 families from a cross between 5R618 and Zhengzhou5389 (susceptible) were inoculated in the greenhouse with the Chinese P. triticina pathotype THJP. Results from the F2 and F2:3 populations indicate that a single dominant gene, temporarily designated Lr5R, conferred resistance. Using the molecular marker method, Lr5R was located on the 3DL chromosome. It was closely linked to the markers Xbarc71 and OPJ-09 with genetic distances of 0.9 cM and 1.0 cM, respectively. At present only one designated gene (Lr24) is located on the 3DL chromosome. The genetic distance between Lr5R and Lr24 confirms that Lr5R is a new leaf rust resistance gene.

Keywords: gene postulation; genetic mapping; molecular marker; wheat leaf rust

Published: December 31, 2014  Show citation

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WANG J, SHI L, ZHU L, Xing L, LIU D. Genetic analysis and molecular mapping of leaf rust resistance genes in the wheat line 5R618. Czech J. Genet. Plant Breed. 2014;50(4):262-267. doi: 10.17221/164/2014-CJGPB.
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    References

    1. Bariana H.S., McIntosh R.A. (1993): Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome, 36: 476-482. Go to original source... Go to PubMed...
    2. Bryan G.J., Collins A.J., Stephenson P., Orry A., Smith J.B., Gale M.D. (1997): Isolation and characterization of microsatellites from hexaploid bread wheat. Theoretical and Applied Genetics, 94: 557-563. Go to original source...
    3. Dubin H.J., Johnson R., Stubbs R.W. (1989): Postulated genes for resistance to strip rust in selected CIMMYT and related wheats. Plant Disease, 73: 472-475. Go to original source...
    4. Gold J., Harder D., Townley-Smith F., Aung T., Procunier J. (1999): Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. Electronic Journal of Biotechnology, 2: 1-2. Go to original source...
    5. Gupta S.K., Charpe A., Koul S., Haque Q., Prabhu K. (2006): Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica, 150: 233-240. Go to original source...
    6. Huerta-Espion J., Singh R.P., German S., McCallum B.D., Park R.F., Chen W.Q., Bhardwaj S.C., Goyeau H. (2011): Global status of wheat leaf rust caused by Puccinia triticina. Euphytica, 179: 143-160. Go to original source...
    7. Kosambi D.D. (1944): The estimation of map distances from recombination values. Annals of Eugenics, 12: 172-175. Go to original source...
    8. Li D., Yuan C.G., Wu H.B., Zhang D., Liang Y., Wang Z.Z., Wu Q.H., Chen Y.X., Yang Z.M., Sun Q.X., Liu Z.Y. (2013): SSR and AFLP-derived SCAR markers associated with the powdery mildew resistance gene in common wheat cultivar ND399. Journal of Plant Genetic Resources, 14: 104-108.
    9. Li X., Li Z.F., Li Y.N., Zhao Z.Q., Liu D.Q., Wang C.F., Gao L.J., Sun D.J. (2010): Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Scientia Agricultura Sinica, 43: 2397-2402.
    10. Long D.L., Kolmer J.A. (1989): A North American system of nomenclature for Puccinia triticina. Phytopathology, 79: 525-529. Go to original source...
    11. Manly F.F., Cudmore R.H., Meer J.M. (2001): Map Manager QTX, crossplatform software for genetic mapping. Mammalian Genome, 12: 930-932. Go to original source... Go to PubMed...
    12. Michelmore R.W., Paran I., Kesseli R.V. (1991): Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 88: 9828-9832. Go to original source... Go to PubMed...
    13. Prins R., Groenewald J.Z., Marais G.F., Snape J.W., Koebner R.M.D. (2001): AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theoretical and Applied Genetics, 103: 618-624. Go to original source...
    14. Roder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. (1998): A microsatellite map of wheat. Genetics, 149: 2007-2023. Go to original source... Go to PubMed...
    15. Sharp P.J., Kreis M., Shewry P.R., Gale M.D. (1988): Location of bamylase sequence in wheat and its relatives. Theoretical and Applied Genetics, 75: 286-290. Go to original source...
    16. Singh D., Mohler V., Park R.F. (2013): Discovery, characterization and mapping of wheat leaf rust resistance gene Lr71. Euphytica, 190: 131-136. Go to original source...
    17. Yuan Z.Y., Wu Y.P., Li Y.F. (2004): Study on validity of genes for leaf rust resistance of wheat in Shanxi. Acta Botany Boreal-Occident Sinica, 24: 1866-1869.
    18. Zhang H., Xia X.C., He Z.H., Li X., Li Z.F., Liu D.Q. (2011): Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai16. Molecular Breeding, 28: 527-534. Go to original source...
    19. Zhang N., Chen Y.T., Li Y.N., Zhang L.R., Meng Q.F., Zhang T., Yang W.X., Liu D.Q. (2008): A novel STS marker for leaf rust resistance gene Lr24 in wheat. Acta Agronomice Sinica, 34: 212-216. Go to original source...
    20. Zhao X.L., Zheng T.C., Xia X.C., He Z.H., Liu D.Q., Yang W.X., Yin G.H., Li Z.F. (2008): Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou8425B. Theoretical and Applied Genetics, 117: 1069-1075. Go to original source... Go to PubMed...
    21. Zhou Y., Wu Y., Li X., Li Z.F., Liu D.Q. (2012): Genetic analysis and molecular mapping of leaf rust resistance genes in two Chinese wheat lines. Scientia Agricultura Sinica, 45: 3273-3380.
    22. Zhou Y., Xia X.C., He Z.H., Li X., Li Z.F., Liu D.Q. (2013): Fine mapping of leaf rust resistance gene LrZH84 using expressed sequence tag and sequence-tagged site markers and allelism with other genes on wheat chromosome 1B. Phytopathology, 103: 169-174. Go to original source... Go to PubMed...

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