Czech J. Genet. Plant Breed., 2013, 49(4):157-163 | DOI: 10.17221/159/2012-CJGPB

Characterization of S haplotype in a new self-compatible Brassica rapa cultivar DahuangyoucaiOriginal Paper

Xingguo ZHANG1, 2, Chaozhi MA2, Dongmei YIN1, Wei ZHU1, Changbin GAO2, Jianfeng ZHANG2, Tingdong FU2
1 College of Agronomy, Henan Agricultural University, Zhengzhou, P.R. China
2 National Key Laboratory of Crop Genetic Improvement, National Centre of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, P.R. China

The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

Keywords: Brassica rapa L.; gene expression; M-locus protein kinase (MLPK); S-locus genes; self-incompatibility

Published: December 31, 2013  Show citation

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ZHANG X, Chaozhi M, YIN D, ZHU W, GAO C, ZHANG J, Tingdong F. Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai. Czech J. Genet. Plant Breed. 2013;49(4):157-163. doi: 10.17221/159/2012-CJGPB.
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    References

    1. Barrett S.C.H. (2002): The evolution of plant sexual diversity. Nature Reviews Genetics, 3: 274-284. Go to original source... Go to PubMed...
    2. Bateman A.J. (1955): Self-incompatibility systems in angiosperms. III. Cruciferae. Heredity, 9: 53-58. Go to original source...
    3. Cabrillac D., Cock J.M., Dumas C., Gaude T. (2001): The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins. Nature, 410: 220-223. Go to original source... Go to PubMed...
    4. Doyle J.J., Doyle J.L. (1990): Isolation of plant DNA from fresh tissue. Focus, 12: 13-15. Go to original source...
    5. Fujimoto R., Sugimura T., Nishio T. (2006): Gene conversion from SLG to SRK resulting in self-compatibility in Brassica rapa. FEBS Letters, 580: 425-430. Go to original source... Go to PubMed...
    6. Goodwillie C., Sargent R.D., Eckert C.G., Elle E., Geber M.A., Johnston M.O., Kalisz S., Moeller D.A., Ree R.H., VallejoMarin M., Winn A.A. (2010): Correlated evolution of mating system and loral display traits in flowering plants and its implications for the distribution of mating system variation. New Phytologist, 185: 311-321. Go to original source... Go to PubMed...
    7. Hinata K., Okazaki K., Nishio K. (1983): Gene analysis of self-compatibility in Brassica campestris var. yellow sarson (a case of recessive epistatic modifier). In: Proc. 6th Int. Rapeseed Congr., Paris, 354-359.
    8. Institute of China Oil Crops Research (1988): List of China Oilseed Varieties. 1st Ed., China Agricultural Press, Beijing. (in Chinese).
    9. Isokawa S., Osaka M., Shirasawa A., Kikuta R., Komatsu S., Horisaki A., Niikura S., Takada Y., Shiba H., Isogai A., Takayama S., Suzuki G., Suwabe K., Watanabe M. (2010): Novel self-compatible of Brassica rapa L. isolated from the Japanese bulk-populations. Genes & Genetic Systems, 85: 87-96. Go to original source... Go to PubMed...
    10. Ivanov R., Gaude T. (2009): Endocytosis and endosomal regulation of the S-receptor kinase during the self-incompatibility response in Brassica oleracea. Plant Cell, 21: 2107-2117. Go to original source... Go to PubMed...
    11. Kakita M., Murase K., Iwano M., Matsumoto T., Watanabe M., Shiba H., Isogai A., Takayama S. (2007): Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signalling in Brassica rapa. Plant Cell, 19: 3961-3973. Go to original source... Go to PubMed...
    12. Murase K., Shiba H., Iwano M., Che F.S., Watanabe M., Isogai A., Takayama S. (2004): A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling. Science, 303: 1516-1519. Go to original source... Go to PubMed...
    13. Nasrallah J.B., Nasrallah M.E. (1993): Pollen-stigma signaling in the sporophytic self-incompatibility response. Plant Cell, 5: 1325-1335. Go to original source... Go to PubMed...
    14. Nasrallah J.B., Yu S.M., Nasrallah M.E. (1988): Selfincompatibility genes of Brassica oleracea: expression, isolation, and structure. Proceedings of the National Academy of Sciences of the USA, 85: 5551-5555. Go to original source... Go to PubMed...
    15. Nasrallah J.B., Nishio T., Nasrallah M.E. (1991): The self-incompatibility genes of Brassica: expression and use in genetic ablation of floral tissues. Annual Review of Plant Physiology and Plant Molecular Biology, 42: 393-422. Go to original source...
    16. Nishio T., Kusaba M., Watanabe M., Hinata K. (1996): Registration of S alleles in Brassica campestris L. by the restriction fragment sizes of SLGs. Theoretical and Applied Genetics, 92: 388-394. Go to original source... Go to PubMed...
    17. Nishio T., Kusaba M., Sakamoto K., Ockendon D.J. (1997): Polymorphism of the kinase domain of the S-locus receptor kinase gene (SRK) in Brassica oleracea L. Theoretical and Applied Genetics, 95: 335-342. Go to original source...
    18. Okamoto S., Odashima M., Fujimoto R., Sato Y., Kitashiba H., Nishio T. (2007): Self-compatibility in Brassica napus is caused by independent mutations in S-locus genes. Plant Journal, 50: 391-400. Go to original source... Go to PubMed...
    19. Rea A.C., Liu P., Nasrallah J.B. (2010) A transgenic self-incompatible Arabidopsis thaliana model for evolutionary and mechanistic studies of crucifer self-incompatibility. Journal of Experimental Botany, 61: 1897-1906. Go to original source... Go to PubMed...
    20. Samuel M.A., Chong Y.T., Haasen K.E., Aldea-Brydges M.G., Stone S.L., Goring D.R. (2009): Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex. Plant Cell, 21: 2655-2671. Go to original source... Go to PubMed...
    21. Schopfer C.R., Nasrallah M.E., Nasrallah J.B. (1999): The male determinant of self-incompatibility in Brassica. Science, 286:1697-1700. Go to original source... Go to PubMed...
    22. Silva N.F., Stone S.L., Christie L.N., Sulaman W., Nazarian K.A.P. (2001): Expression of the S receptor kinase in self-compatible Brassica napus cv. Westar leads to the allele-specific rejection of self-incompatible Brassica napus pollen. Molecular Genetics and Genomics, 265: 552-559. Go to original source... Go to PubMed...
    23. Stein J.C., Howlett B., Boyes D.C., Nasrallah M.E., Nasrallah J.B. (1991): Molecular cloning of a putative rector protein kinase encoded at the self-incompatibility locus of Brassia oleracea. Proceedings of the National Academy of Sciences of the USA, 88: 8816-8820. Go to original source... Go to PubMed...
    24. Stone S.L., Anderson E.M., Mullen R.T., Goring D.R. (2003): ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of selfincompatible Brassica pollen. Plant Cell, 15: 885-898. Go to original source... Go to PubMed...
    25. Suzuki G., Kai N., Hirose T., Fukai K., Nishio T., Takayama S., Isogai A., Watanabe M., Hinata K. (1999): Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S(9) haplotype of Brassica campestris (syn. rapa). Genetics, 153: 391-400. Go to original source... Go to PubMed...
    26. Takasaki T., Hatakeyama K., Suzuki G., Watanabe M., Isogai A., Hinata K. (2000): The S receptor kinase determines self-incompatibility in Brassica stigma. Nature, 403: 913-916. Go to original source... Go to PubMed...
    27. Takayama S., Isogai A. (2005): Self-incompatibility in plants. Annual Review of Plant Biology, 56: 467-489. Go to original source... Go to PubMed...
    28. Tang G.Y., Xu Q.L., Wang R.S., Li j., Du D.Z. (2006): Primary study on self-compatibility in Dahuang Rape of Brassica chinensis. Science and Technology of Qinghai Agriculture, 1: 8-9 (in Chinese)
    29. Tantikanjana T., Rizvi N., Nasrallah M.E., Nasrallah J.B. (2009): A dual role for the S-locus receptor kinase in self-incompatibility and pistil development revealed by an Arabidopsis rdr6 mutation. Plant Cell, 21: 2642-2654. Go to original source... Go to PubMed...
    30. Zhang X.G., Ma C.Z., Fu T.D., Li Y.Y., Wang T.H., Chen Q.F., Tu J.X., Shen J.X. (2008): Development of SCAR markers linked to self-incompatibility in Brassica napus L. Molecular Breeding, 21: 305-315. Go to original source...

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