Czech J. Genet. Plant Breed., 2011, 47(4):166-171 | DOI: 10.17221/42/2011-CJGPB

Characterisation of oat genetic resources using electrophoresis of aveninsShort Communication

Ivana POLIŠENSKÁ1, Lenka NEDOMOVÁ2, Tomáš VYHNÁNEK3
1 Agrotest Fyto, Ltd., Kroměříž, Czech Republic
2 Agricultural Research Institute Kromeriz, Ltd., Kroměříž, Czech Republic
3 Mendel University in Brno, Brno, Czech Republic

The prolamin (avenin) patterns of oat (Avena sativa L.) cultivars released in the Czech Republic and in the former Czechoslovak Republic were analysed by acid polyacrylamide gel electrophoresis (A-PAGE). Forty-nine oat (Avena sativa L.) accessions of domestic origin, maintained in the Czech collection of oat genetic resources, were analysed. The evaluated set contained 18 modern and 31 old cultivars. Thirty accessions showed a homogeneous prolamin pattern. The other accessions were heterogeneous with two or three different patterns, present at different percentages. Heterogeneity was present in 48% of the old cultivars, but only in 22% of the modern cultivars. Identity indexes within the heterogeneous accessions were calculated. The index values ranged from 0.09 to 0.75. Only in two heterogeneous cultivars (Dětenický Bílý, Valečovský Bílý) the identity index between their components was higher than 0.6, indicating, that their components were most likely sister lines. All analysed cultivars could be unambiguously distinguished by their prolamin pattern. The obtained prolamin patterns will be used to complete descriptor data of the genetic resources and might be useful also in oat breeding and research.

Keywords: avenins; genotype distinguishing; line; oats; PAGE

Published: December 31, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
POLIŠENSKÁ I, NEDOMOVÁ L, VYHNÁNEK T. Characterisation of oat genetic resources using electrophoresis of avenins. Czech J. Genet. Plant Breed. 2011;47(4):166-171. doi: 10.17221/42/2011-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bradová J., Matějová E. (2008): Comparison of the results of SDS PAGE and chip electrophoresis of wheat storage proteins. Chromatographia, 67: S83-S88. Go to original source...
    2. Černý J., Šašek A. (1998): Using of Electroforetical BGM Analysis for Characterisation of Wheat and Barley Varieties. ÚZPI Praha. (in Czech)
    3. ČSN 46 1085-2 (1998): Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) - Determination of Varietal Trueness and Purity - Part 2: Protein Polyacrylamide Gel Electrophoresis (PAGE). Český normalizační institut, Praha. (in Czech)
    4. Dvořáček V., Čurn V., Moudrý J. (2003): Suitability of oat-seed storage-protein markers for identification of cultivar in grain and mixed flour samples. Plant, Soil and Environment, 49: 486-491. Go to original source...
    5. Echart-Almeid C., Cavalli-Molina S. (2000): Hordein variation in Brazilian barley varieties (Hordeum vulgare L.) and wild barley (H. eulaston Steud. and H. stenostachys Godr.). Genetics and Molecular Biology, 23: 425-433. Go to original source...
    6. Gregová E., Longauer I., Žák I., Kraic J. (1996): Electrophoretic distinguishing of cultivated oats (Avena sativa L.) by seed storage proteins. Rostlinná Výroba, 42: 169-172.
    7. Hadačová E., Turková V., Hadač E., Klozová E. (1980): Comparison of seed proteins of some representatives of the genus Pisum from the point of view of their relationship. Biologia Plantarum, 22: 7-16. Go to original source...
    8. ISTA (1999): Verification of species and cultivars. Chapter VIII. In: International Rules for Seed Testing. ISTA, Bassersdorf, 41-43, 252-254.
    9. Mohammadi S.A., Prasanna B.M. (2003): Analysis of genetic diversity in crop plants - salient statistical tools and considerations. Crop Science, 43: 1235-1248. Go to original source...
    10. Morikawa T., Arase H. (1999): Polymorphism of seed storage protein (Avenin) in cultivated oats. Breeding Research, 1: 177.
    11. Polišenská I., Nedomová L., Cupák S. (2010): Charakterizace genotypů ovsa s využitím elektroforézy aveninů v polyakrylamidovém gelu (A-PAGE). Metodika. Zemědělský výzkumný ústav Kroměříž, s.r.o., Agrotest fyto, s.r.o., Kroměříž.
    12. Pomortsev A.A. (2 1): Hordein polymorphism in Ethiopian barley. Russian Journal of Genetics, 37: 1150-1160. Go to original source...
    13. Portyanko V.A., Sharopova N.R., Sozinov A.A. (1998): Characterisation of European oat germ plasm: allelic variation at complex avenin loci detected by acid polyacrylamide gel electrophoresis. Euphytica, 102: 15-27. Go to original source...
    14. Šašek A., Černý J., Bradová J. (1998): Electrophoretic spectra of gliadins and high-molecular-weight subunits of gluteins in common wheat varieties registered in 1996 and 1997. Czech Journal of Genetics and Plant Breeding, 34: 95-101.
    15. Šašek A., Černý J., Hanišová A. (1982): The use of gliadin markers in wheat breeding. Genetika a šlechtění, 18: 241-255.
    16. Vyhnánek T., Bednář J. (2003): Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker. Plant, Soil and Environment, 49: 95-98. Go to original source...
    17. Vyhnánek T., Bednář J., Helánová S., Nedomová L., Milotová J. (2003): Use of prolamin polymorphism to describe genetic variation in a collection of barley genetic resources. Czech Journal of Genetics and Plant Breeding, 39: 45-50. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content