Czech J. Genet. Plant Breed., 2009, 45(2):79-84 | DOI: 10.17221/28/2009-CJGPB

Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech RepublicShort Communication

Alena HANZALOVÁ, Ta»ána SUMÍKOVÁ, Pavel BARTO©
Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Twenty-seven winter wheat cultivars registered in the Czech Republic were tested by molecular markers for the presence of Lr26 and Lr37, and twenty-eight cultivars for the presence of Lr10. Gene Lr37 was determined in eleven cultivars, gene Lr10 in ten cultivars and gene Lr26 in four cultivars. Eight cultivars had combinations of two Lr genes, one cultivar possessed all the three Lr genes. The results of marker analyses were compared with multipathotype analysis which confirmed the presence of Lr26 but did not enable the verification of the presence of Lr10 and Lr37. Seedling resistance was compared with resistance of the studied cultivars in the field.

Keywords: wheat leaf rust; Puccinia triticina; rust resistance; genes Lr10, Lr26 and Lr37; molecular markers; registered wheat cultivars; Czech Republic

Published: June 30, 2009  Show citation

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HANZALOVÁ A, SUMÍKOVÁ T, BARTO© P. Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech Republic. Czech J. Genet. Plant Breed. 2009;45(2):79-84. doi: 10.17221/28/2009-CJGPB.
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References

  1. Ambrozková M., Dedryver F., Dumalasová V., Hanzalová A., Bartoą P. (2002): Determination of the cluster of wheat rust resistance genes Yr17, Lr37 and Sr38 by a molecular marker. Plant Protection Science, 38: 41-45. Go to original source...
  2. Bartoą P., Ovesná J., Hanzalová A., Chrpová J., Dumalasová V., ©korpík M., ©íp V. (2004): Presence of a translocation from Aegilops ventricosa in wheat cultivars registered in the Czech Republic. Czech Journal of Genetics and Plant Breeding, 40: 31-35. Go to original source...
  3. Blaszczyk L., Goyeau H., Huang X.-Q., Röder M., Stepień L., Chelkowski J. (2004): Identifying leaf rust resistance genes and mapping gene Lr37 on the microsatellite map of wheat. Cellular and Molecular Biology Letters, 9: 869-878.
  4. Blaľková V., Bartoą P., Park R., Goyeau H. (2002): Verifying the presence of leaf rust resistance gene Lr10 in sixteen wheat cultivars by use of PCR-based STS marker. Cereal Research Communications, 30: 9-16. Go to original source...
  5. Bradová J., ©aąek A. (2007): Optimization of Methods of Protein Electrophoresis for the Identification of Wheat Cultivars (Triticum aestivum L.). VÚRV, Praha.
  6. De Froidmont D. (1998): A co-dominant marker for the 1BL.1RS wheat-rye translocation via multiplex PCR. Journal of Cereal Science, 27: 229-232. Go to original source...
  7. Feuillet C., Schachermayr G., Keller B. (1997): Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. The Plant Journal, 11: 45-52. Go to original source... Go to PubMed...
  8. Helguera M., Khan I.A., Kolmer J., Lijavetzky D, Zhong-Gi L., Dubcovsky J. (2003): PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Science, 43: 1839-1847. Go to original source...
  9. Landjeva S., Korzun V., Börner A. (2007): Molecular markers: actual and potential contributions to wheat genome characterization and breeding. Euphytica, 156: 271-296. Go to original source...
  10. McIntosh R.A., Wellings C.R., Park R.F. (1995): Wheat Rusts: An Atlas of Resistance Genes. CSIRO, Melbourne. Go to original source...
  11. Schachermayr G., Feuillet C., Keller B. (1997): Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Molecular Breeding, 3: 65-74. Go to original source...
  12. Stakman E.C., Stewart D.M., Loegering W.Q. (1962): Identification of physiological races of Puccinia graminis var. tritici. Agricultural Research Service E 617, US Department of Agriculture, Washington.
  13. Stepieň L., Golka L., Chelkowski J. (2003): Leaf rust resistance genes of wheat: identification in cultivars and resistance sources. Journal of Applied Genetics, 44: 139-149. http://maswheat.ucdavis.edu/protocols

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