Czech J. Genet. Plant Breed., 2007, 43(3):105-112 | DOI: 10.17221/2071-CJGPB

Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers

Chengyu YU1, Leona Leišová2, Vratislav Kučera2, Miroslava Vyvadilová2, Jaroslava Ovesná2, Ladislav Dotlačil2, Shengwu HU1
1 College of Agronomy, Northwest A&F University, Yangling, China
2 Crop Research Institute, Prague-Ruzyně, Czech Republic

The genetic diversity of 35 yellow-seeded Brassica napus L. accessions originating from China, Czech Republic and Poland was assessed by means of Amplified Fragment Length Polymorphism (AFLP) markers based on multiplex PCR using multi-colour fluorescent-labelled primers. Five brown-seeded accessions originating from China and France were selected as outliers. In total, 632 peaks were generated by AFLP reaction using 18 primer combinations. Only distinctly polymorphic markers among them were scored. In total, 242 polymorphic markers were detected with an average of 13.4 markers per primer combination. The AFLP analysis separated forty studied accessions into Chinese and European groups by UPGMA clustering and Principal Coordinates Analysis (PCA). The grouping of accessions based on the cluster analysis and PCA was generally consistent with known pedigree information and geographic origin. Notable geographical divergence was found between Chinese and European yellow-seeded accessions. This information is useful for yellow-seeded hybrid breeding and encouraging breeders to exchange their germplasm as to enlarge the genetic diversity of breeding accessions.

Keywords: AFLP; Brassica napus L.; genetic diversity; yellow seed trait

Published: September 30, 2007  Show citation

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Chengyu Y, Leišová L, Kučera V, Vyvadilová M, Ovesná J, Dotlačil L, Shengwu H. Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers. Czech J. Genet. Plant Breed. 2007;43(3):105-112. doi: 10.17221/2071-CJGPB.
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    References

    1. Chen B., Heneen S.K., Jonsson R. (1988): Resynthesis of Brassica napus L. through interspecific hybridization between B. alboglabra Bailey and B. campestris L. with special emphasis on seed colour. Plant Breeding, 101: 52-59. Go to original source...
    2. Doyle J.J., Dickson E.E. (1987): Preservation of plant samples for DNA restriction endonuclease analysis. Taxon, 36: 715-722. Go to original source...
    3. Hu S.W., Ovesná J., Kučera L., Kučera V., Vyvadilová M. (2003): Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers. Plant, Soil and Environment, 49: 106-113. Go to original source...
    4. Li D.R., Tian J.H. (1999): Discovery of dominant yellow-seeded genes and the breeding of yellow-seeded hybrid in Brassica napus L. In: 10 th Int. Rapeseed Congr., Canberra, 25.
    5. Li J.N., Zhang X.K., Chen L., Wang R., Cui C. (1998): Initial study on the genetics of seed colour of the yellow-seeded lines (Brassica napus L.) from different genetic sources. Chinese Journal of Oil Crops Science, 20: 16-19.
    6. Liu H.L. (1992): Studies on the inheritance of yellowseeded Brassica napus L. Acta Agronomica Sinica, 18: 241-249.
    7. Lombard V., Baril C.P., Dubreuil P., Blouet F., Zhang D. (2000): Genetic relationships and finger printing of rape seed cultivars by AFLP: Consequences for varietal registration. Crop Science, 40: 1417-1425. Go to original source...
    8. Meng J.L., Shi S.W., Gan L., Li Z.Y., Qu X.S. (1998): The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica, 103: 329-333. Go to original source...
    9. Nei M., Li W.H. (1979): Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA, 76: 5269-5273. Go to original source... Go to PubMed...
    10. Rashid A., Rakow G., Downey R.K. (1994): Development of yellow-seeded Brassica napus through interspecific crosses. Plant Breeding, 112: 127-134. Go to original source...
    11. Rohlf F.J. (2001): NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc., Setauket, New York.
    12. Seyis F., Snowdon R.J., Lühs W., Friedt W. (2003): Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars. Plant Breeding, 122: 473-478. Go to original source...
    13. Shirzadegan M. (1986): Inheritance of seed colour in Brassica napus L. Zeitschrift für Pflanzenzüchtung, 96: 140-146.
    14. Sobotka R., Dolanská L., čurn V., Ovesná J. (2004): Fluorescence-based AFLPs occur as the most suitable marker system for oilseed rape cultivar identification. Journal of Applied Genetics, 45: 161-173. Go to PubMed...
    15. Stringam G.R., McGregor D.I., Pawlowski S.H. (1974): Chemical and morphological characteristics associated with seed coat colour in rapeseed. In: Proc. 4th Int. Rapeseed Congr., Giessen, 99-108.
    16. Vyvadilová M., Tomášková D., Bechyně M., Kučera V. (1999): The use of doubled haploids to stabilize yellow-seedness in oilseed rape (Brassica napus). Czech Journal of Genetics and Plant Breeding, 35: 7-9.
    17. Yu C.Y., Hu S.W., Zhao H.X., Guo A.G., Sun G.L. (2005): Genetic distances revealed by morphological characters, isozymes, proteins and RAPD markers and their relationships with hybrid performance in oilseed rape (Brassica napus L.). Theoretical and Applied Genetics, 110: 511-518. Go to original source... Go to PubMed...
    18. Zhang X.K., Chen L., Yin J.M., Tang Z.L., Li J.N. (2003): Genetic diversity of RAPD marker and testa pigments of yellow-seeded rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 36: 752-756.

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