Czech J. Genet. Plant Breed., 2006, 42(10):72-74 | DOI: 10.17221/6236-CJGPB
Development of immunochemical and PCR methods for qualitative detection of Tilletia species in organic seeds
- 1 Bavarian State Research Center for Agriculture, Institute for Crop Science and Plant Breeding, Working Group of Seed Research, Freising, Germany
- 2 Federal Centre for Breeding Research on Cultivated Plants, Institute of Resistance Research and Pathogen Diagnostics, Aschersleben, Germany, e-mail: Berta.Killermann@lfl.bayern.de
Keywords: seed-borne fungi; Tilletia species; Western Blot; PCR; polyclonal antibodies
Published: December 31, 2006 Show citation
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Kellerer T, Sedlmeier M, Rabenstein F, Killermann B. Development of immunochemical and PCR methods for qualitative detection of Tilletia species in organic seeds. Czech Journal of Genetics and Plant Breeding. 2006;42(Special Issue):72-74. doi: 10.17221/6236-CJGPB.
References
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- Gang D.R., Weber D.J. (1995): Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores of Tilletia species. BioTechniques, 19: 92-96.
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- van Etten J., Freer S. (1978): Simple Procedure for Disruption of Fungal Spores. Applied and Environmental Microbiology, 622-623. 74
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