Czech J. Genet. Plant Breed., 2006, 42(10):72-74 | DOI: 10.17221/6236-CJGPB

Development of immunochemical and PCR methods for qualitative detection of Tilletia species in organic seeds

T. Kellerer1, M. Sedlmeier1, F. Rabenstein2, B. Killermann1
1 Bavarian State Research Center for Agriculture, Institute for Crop Science and Plant Breeding, Working Group of Seed Research, Freising, Germany
2 Federal Centre for Breeding Research on Cultivated Plants, Institute of Resistance Research and Pathogen Diagnostics, Aschersleben, Germany, e-mail: Berta.Killermann@lfl.bayern.de

Keywords: seed-borne fungi; Tilletia species; Western Blot; PCR; polyclonal antibodies

Published: December 31, 2006  Show citation

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Kellerer T, Sedlmeier M, Rabenstein F, Killermann B. Development of immunochemical and PCR methods for qualitative detection of Tilletia species in organic seeds. Czech J. Genet. Plant Breed. 2006;42(Special Issue):72-74. doi: 10.17221/6236-CJGPB.
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References

  1. Ferreira A., Glass N. (1996): PCR from fungal spores after microwave treatment. http://www.fgsc.net/fgn43/ferreir.html
  2. Gang D.R., Weber D.J. (1995): Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores of Tilletia species. BioTechniques, 19: 92-96. Go to PubMed...
  3. Sulc M., Ulrych A., Jegorov A., Zabka M., Havlicek V. (2005): Exploring fungal spore proteins by mass spectrometry. http://ms.biomed.cas.cz/downloads/Sulc_HUPO_05.pdf
  4. van Etten J., Freer S. (1978): Simple Procedure for Disruption of Fungal Spores. Applied and Environmental Microbiology, 622-623. 74 Go to original source... Go to PubMed...

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